Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,855,960 C→T S4N (AGC→AAC) 
*116* (TAG→TAA) 		 yidG ←
yidH ←		 inner membrane protein
DUF202 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,855,9600CT100.0% 258.1 / NA 79S4N (AGC→AAC) 
*116* (TAG→TAA) 		yidG
yidH		inner membrane protein
DUF202 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/45);  total (34/45)

CATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAG  >  NC_000913/3855827‑3856095
                                                                                                                                     |                                                                                                                                       
cATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATcc                                                                                                                                    >  1:1090267/1‑139 (MQ=255)
  tGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCgg                                                                                                                                  <  2:829885/139‑1 (MQ=255)
  tGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCgg                                                                                                                                  >  1:829885/1‑139 (MQ=255)
      cGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATa                                                                                                                              >  1:1214215/1‑139 (MQ=255)
       gCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATac                                                                                                                             <  2:1244531/139‑1 (MQ=255)
       gCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATac                                                                                                                             <  2:456438/139‑1 (MQ=255)
       accTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATac                                                                                                                             <  2:357957/138‑1 (MQ=255)
            gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTATGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        <  1:391448/139‑1 (MQ=255)
            gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        <  2:1090267/139‑1 (MQ=255)
            gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        <  2:1018754/139‑1 (MQ=255)
            gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        <  1:45789/139‑1 (MQ=255)
                   ttGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTccc                                                                                                                 >  2:1351267/1‑139 (MQ=255)
                   ttGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTccc                                                                                                                 <  1:1133685/139‑1 (MQ=255)
                   ttGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTccc                                                                                                                 <  1:361537/139‑1 (MQ=255)
                           gATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGAt                                                                                                         >  1:584399/1‑139 (MQ=255)
                            aTAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATc                                                                                                        >  2:68974/1‑139 (MQ=255)
                             tAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCgg                                                                                                                                  >  2:994642/1‑112 (MQ=255)
                             tAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCgg                                                                                                                                  <  1:994642/112‑1 (MQ=255)
                             tAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCCGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCa                                                                                                       >  1:736236/1‑139 (MQ=255)
                                   aCGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCa                                                                                                 <  1:794742/139‑1 (MQ=255)
                                   aCGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCa                                                                                                 <  1:751438/139‑1 (MQ=255)
                                    cGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAg                                                                                                                     >  2:595068/1‑118 (MQ=255)
                                    cGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAg                                                                                                                     <  1:595068/118‑1 (MQ=255)
                                     gCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACAcc                                                                                                                       <  1:4901/115‑1 (MQ=255)
                                     gCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACAcc                                                                                                                       >  2:4901/1‑115 (MQ=255)
                                     gCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCCGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                               <  2:736236/139‑1 (MQ=255)
                                         tCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATac                                                                                                                             >  2:675249/1‑105 (MQ=255)
                                         tCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATac                                                                                                                             <  1:675249/105‑1 (MQ=255)
                                          cAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                          <  2:1214215/139‑1 (MQ=255)
                                               cgcCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAata                                                                                     >  2:150563/1‑139 (MQ=255)
                                                 ccGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                          >  1:1032188/1‑132 (MQ=255)
                                                 ccGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                          <  2:1032188/132‑1 (MQ=255)
                                                  cGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTa                                                                                  <  1:68974/139‑1 (MQ=255)
                                                       cccAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTa                                                                             >  1:808829/1‑139 (MQ=255)
                                                            cATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATa                                                                                                                              <  2:99046/85‑1 (MQ=255)
                                                            cATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATa                                                                                                                              >  1:99046/1‑85 (MQ=255)
                                                            cATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGAt                                                                        >  1:554738/1‑139 (MQ=255)
                                                              tGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        >  2:1124791/1‑89 (MQ=255)
                                                              tGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        <  1:1124791/89‑1 (MQ=255)
                                                               ggTATGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        <  1:759670/88‑1 (MQ=255)
                                                               ggTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        >  2:759670/1‑88 (MQ=255)
                                                                 tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTc                                                                                                                   >  2:946164/1‑91 (MQ=255)
                                                                 tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTc                                                                                                                   <  1:946164/91‑1 (MQ=255)
                                                                 tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGcc                                                                                                  <  1:902291/108‑1 (MQ=255)
                                                                 tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACCATCACCGcc                                                                                                  >  2:902291/1‑108 (MQ=255)
                                                                       aCCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                             <  2:808829/139‑1 (MQ=255)
                                                                       aCCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                             <  1:501326/139‑1 (MQ=255)
                                                                       aCCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                             <  2:584399/139‑1 (MQ=255)
                                                                       aCCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                             <  2:379137/139‑1 (MQ=255)
                                                                          aCGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCAc                                                                                                      >  1:718524/1‑95 (MQ=255)
                                                                          aCGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCAc                                                                                                      <  2:718524/95‑1 (MQ=255)
                                                                           cGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATta                                                                      <  1:1143641/126‑1 (MQ=255)
                                                                           cGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATta                                                                      >  2:1143641/1‑126 (MQ=255)
                                                                                   aTGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCa                                                 >  1:172720/1‑139 (MQ=255)
                                                                                   aTGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCa                                                 <  1:1351267/139‑1 (MQ=255)
                                                                                          cTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATc                                                                                         >  1:958542/1‑92 (MQ=255)
                                                                                          cTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATc                                                                                         <  2:958542/92‑1 (MQ=255)
                                                                                             cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                          >  2:439108/1‑88 (MQ=255)
                                                                                             cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                          <  1:439108/88‑1 (MQ=255)
                                                                                                             tCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCtttt                       >  2:524635/1‑139 (MQ=255)
                                                                                                               ggCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTc                     <  2:172720/139‑1 (MQ=255)
                                                                                                                gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        <  2:1310351/39‑1 (MQ=255)
                                                                                                                gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                        >  1:1310351/1‑39 (MQ=255)
                                                                                                                gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                               <  2:458525/64‑1 (MQ=255)
                                                                                                                gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                               >  1:458525/1‑64 (MQ=255)
                                                                                                                gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGc                                                                               >  1:1195568/1‑80 (MQ=255)
                                                                                                                gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGc                                                                               <  2:1195568/80‑1 (MQ=255)
                                                                                                                gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTc                                             >  1:622674/1‑114 (MQ=255)
                                                                                                                gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTc                                             <  2:622674/114‑1 (MQ=255)
                                                                                                                gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCa                    >  2:501597/1‑139 (MQ=255)
                                                                                                                           ggCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCa         >  1:1186980/1‑139 (MQ=255)
                                                                                                                             cTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAgc       <  1:501597/139‑1 (MQ=255)
                                                                                                                                tGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCa    <  2:1186980/139‑1 (MQ=255)
                                                                                                                                tGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCa    <  1:150563/139‑1 (MQ=255)
                                                                                                                                 gCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGAc                                                                                             <  1:216591/49‑1 (MQ=255)
                                                                                                                                 gCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGAc                                                                                             >  2:216591/1‑49 (MQ=255)
                                                                                                                                 gCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGc                                                                               >  2:240651/1‑63 (MQ=255)
                                                                                                                                 gCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGc                                                                               <  1:240651/63‑1 (MQ=255)
                                                                                                                                  cGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAg  <  1:524635/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
CATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAG  >  NC_000913/3855827‑3856095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑