Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	354,592	G→A	*629* (TAG→TAA)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,592	0	G	A	100.0%	211.4 / NA	65	*629* (TAG→TAA)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/29);  total (36/29)

AAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATG  >  NC_000913/354479‑354726                                                                                                                  |                                                                                                                                       aaaaTGCTGCGCCGCACGATCCAGGCGATCTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCTTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  2:924070/139‑1 (MQ=255)               cACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACtgt                                                                                                 >  2:1179102/1‑139 (MQ=255)                 cGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACtgtgt                                                                                               >  2:4041/1‑139 (MQ=255)                              ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGt                                                                                                                                >  1:290855/1‑93 (MQ=255)                              ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGt                                                                                                                                <  2:290855/93‑1 (MQ=255)                              ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  1:985770/1‑139 (MQ=255)                              ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  2:1059540/1‑139 (MQ=255)                              ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  1:240911/1‑139 (MQ=255)                              ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  2:537750/1‑139 (MQ=255)                                                tGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               >  1:121374/1‑92 (MQ=255)                                                tGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  2:121374/92‑1 (MQ=255)                                                     aTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt                                                           >  1:1254982/1‑139 (MQ=255)                                                     aTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt                                                           <  1:4041/139‑1 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCg                                                                                           <  2:854137/105‑1 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGa                                                                        <  1:1379441/124‑1 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGa                                                                        >  2:1379441/1‑124 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATc                                                             >  1:829852/1‑135 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc                                                         >  2:1281867/1‑139 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc                                                         >  2:992821/1‑139 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCCGCACTGTGTGCCg                                                                                           >  1:854137/1‑105 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTCCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGt                                                                   >  2:1119996/1‑129 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTCCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGt                                                                   <  1:1119996/129‑1 (MQ=255)                                                       cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCAGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTtctc                                                             <  2:829852/135‑1 (MQ=255)                                                        tGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                        >  1:938841/1‑139 (MQ=255)                                                          aggacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                      <  2:1396366/137‑1 (MQ=255)                                                            gacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACg                                                    <  2:938841/139‑1 (MQ=255)                                                               cATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGGTTTATCATGCCTACGGAc                                                 <  1:1059540/139‑1 (MQ=255)                                                                    atgatCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGaa                                            >  2:977437/1‑139 (MQ=255)                                                                        tCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGt                                        <  1:1281867/139‑1 (MQ=255)                                                                         ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTcgcg                                                                                                                   >  1:152209/1‑63 (MQ=255)                                                                         ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTcgcg                                                                                                                   <  2:152209/63‑1 (MQ=255)                                                                         ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     >  1:716578/1‑77 (MQ=255)                                                                         ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     <  2:716578/77‑1 (MQ=255)                                                                           tGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAgg                                     >  2:1138328/1‑139 (MQ=255)                                                                            gCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGt                                    >  1:441627/1‑139 (MQ=255)                                                                            gCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGt                                    <  2:240911/139‑1 (MQ=255)                                                                             cgtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCt                                               <  2:1198702/127‑1 (MQ=255)                                                                             cgtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCt                                               >  1:1198702/1‑127 (MQ=255)                                                                             cgtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTc                                   <  1:537750/139‑1 (MQ=255)                                                                              gtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGtttt                                                                >  1:204010/1‑109 (MQ=255)                                                                              gtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGtttt                                                                <  2:204010/109‑1 (MQ=255)                                                                                  ttGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCgg                                                                                                       <  2:1373069/66‑1 (MQ=255)                                                                                  ttGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCgg                                                                                                       >  1:1373069/1‑66 (MQ=255)                                                                                  ttGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     >  2:427744/1‑68 (MQ=255)                                                                                  ttGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     <  1:427744/68‑1 (MQ=255)                                                                                  ttGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGa                                                                        <  2:375544/97‑1 (MQ=255)                                                                                  ttGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGa                                                                        >  1:375544/1‑97 (MQ=255)                                                                                  ttGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATa                              >  2:962411/1‑139 (MQ=255)                                                                                   tGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATaa                             >  1:691727/1‑139 (MQ=255)                                                                                          gATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTc                      >  1:887395/1‑139 (MQ=255)                                                                                           aTCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                       >  2:127589/1‑89 (MQ=255)                                                                                           aTCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                       <  1:127589/89‑1 (MQ=255)                                                                                             ccGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTcgcg                   >  1:735519/1‑139 (MQ=255)                                                                                              cGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGt                  <  2:735519/139‑1 (MQ=255)                                                                                               gCCAGGCGATGGAAGAGTAAGTTAGTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                 >  2:969170/1‑139 (MQ=255)                                                                                                ccAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCg                >  2:413375/1‑139 (MQ=255)                                                                                                    gCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATc            >  2:837467/1‑139 (MQ=255)                                                                                                       aTGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCg                >  1:457295/1‑132 (MQ=255)                                                                                                       aTGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCg                <  2:457295/132‑1 (MQ=255)                                                                                                        tGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGac        <  2:691727/139‑1 (MQ=255)                                                                                                         ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGt                  >  2:388099/1‑128 (MQ=255)                                                                                                         ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGt                  <  1:388099/128‑1 (MQ=255)                                                                                                         ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       <  1:969170/139‑1 (MQ=255)                                                                                                         ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       <  1:977437/139‑1 (MQ=255)                                                                                                              agTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATg  <  2:985770/139‑1 (MQ=255)                                                                                                                  |                                                                                                                                       AAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATG  >  NC_000913/354479‑354726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑