Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,441 G→A *355* (TAG→TAA
G430G (GGC→GGT
cbrA →
dgoT ←
colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,4410GA100.0% 161.1 / NA 50*355* (TAG→TAA
G430G (GGC→GGT
cbrA
dgoT
colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

GCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGG  >  NC_000913/3870316‑3870571
                                                                                                                             |                                                                                                                                  
gcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTca                                                                                                                       <  2:808860/139‑1 (MQ=255)
       gCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                                <  2:634028/139‑1 (MQ=255)
               cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                        <  2:1246460/139‑1 (MQ=255)
               cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                        >  2:248793/1‑139 (MQ=255)
                 tttAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAcgcg                                                                                                                            <  1:439410/117‑1 (MQ=255)
                 tttAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAcgcg                                                                                                                            >  2:439410/1‑117 (MQ=255)
                         tctTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAaga                                                                                              >  2:1200921/1‑139 (MQ=255)
                           ttCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                      <  2:1118809/129‑1 (MQ=255)
                           ttCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                      >  1:1118809/1‑129 (MQ=255)
                              ggCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcg                                                                                         <  2:1353355/139‑1 (MQ=255)
                              ggCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcg                                                                                         >  1:1353355/1‑139 (MQ=255)
                                     tAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCg                                                                                                                  >  2:1170988/1‑107 (MQ=255)
                                     tAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCg                                                                                                                  <  1:1170988/107‑1 (MQ=255)
                                             aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCaa                                                                                                                                >  2:782339/1‑85 (MQ=255)
                                             aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCaa                                                                                                                                <  1:782339/85‑1 (MQ=255)
                                             aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                        >  2:674393/1‑109 (MQ=255)
                                             aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                        <  1:674393/109‑1 (MQ=255)
                                                cGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCGCATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                          <  1:1266026/104‑1 (MQ=255)
                                                cGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCGCATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                          >  2:1266026/1‑104 (MQ=255)
                                                                 ggCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCccac                                                                                                             >  1:239487/1‑84 (MQ=255)
                                                                 ggCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCccac                                                                                                             <  2:239487/84‑1 (MQ=255)
                                                                           aaGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                            <  1:390656/139‑1 (MQ=255)
                                                                                        gcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTg                               <  2:682897/139‑1 (MQ=255)
                                                                                            tggtgTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGcc                           <  1:1188536/139‑1 (MQ=255)
                                                                                                 tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                            >  2:1066433/1‑117 (MQ=255)
                                                                                                 tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                            <  1:1066433/117‑1 (MQ=255)
                                                                                                      caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                    >  2:363891/1‑88 (MQ=255)
                                                                                                      caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                    <  1:363891/88‑1 (MQ=255)
                                                                                                      caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                            >  1:1267006/1‑112 (MQ=255)
                                                                                                      caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                            <  2:1267006/112‑1 (MQ=255)
                                                                                                      caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCcca                 >  1:608793/1‑139 (MQ=255)
                                                                                                        tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                        <  2:30043/50‑1 (MQ=255)
                                                                                                        tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                        >  1:30043/1‑50 (MQ=255)
                                                                                                           tCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                                <  2:923009/39‑1 (MQ=255)
                                                                                                           tCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                                >  1:923009/1‑39 (MQ=255)
                                                                                                             cacaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                          <  2:287773/43‑1 (MQ=255)
                                                                                                             cacaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                          >  1:287773/1‑43 (MQ=255)
                                                                                                              acaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGc         >  1:642701/1‑139 (MQ=255)
                                                                                                                    gAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                    >  1:647658/1‑74 (MQ=255)
                                                                                                                    gAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                    <  2:647658/74‑1 (MQ=255)
                                                                                                                     aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcgc                                                                                      <  2:1390246/55‑1 (MQ=255)
                                                                                                                     aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcgc                                                                                      >  1:1390246/1‑55 (MQ=255)
                                                                                                                     aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcg                                                                                       <  2:1350005/54‑1 (MQ=255)
                                                                                                                     aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcg                                                                                       >  1:1350005/1‑54 (MQ=255)
                                                                                                                     aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                    >  1:366228/1‑73 (MQ=255)
                                                                                                                     aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                    <  2:366228/73‑1 (MQ=255)
                                                                                                                     aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTg                               <  2:256492/110‑1 (MQ=255)
                                                                                                                     aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTg                               >  1:256492/1‑110 (MQ=255)
                                                                                                                     aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACgg  >  1:967936/1‑139 (MQ=255)
                                                                                                                          ttAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCCCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGcc                           >  1:1132089/1‑109 (MQ=255)
                                                                                                                             |                                                                                                                                  
GCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGG  >  NC_000913/3870316‑3870571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: