Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,024,336	G→A	*261* (TAG→TAA)  L162L (CTC→CTT)	tatD → rfaH ←	quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase transcription antitermination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,024,336	0	G	A	100.0%	211.7 / NA	66	*261* (TAG→TAA)  L162L (CTC→CTT)	tatD rfaH	quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase transcription antitermination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/37);  total (29/37)

CCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGG  >  NC_000913/4024206‑4024466                                                                                                                                   |                                                                                                                                   cccGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCg                                                                                                                            <  2:1131893/139‑1 (MQ=255)   cGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGa                                                                                                                          <  2:537352/139‑1 (MQ=255)        cAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                                     >  2:107980/1‑139 (MQ=255)        cAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                                     <  1:1099237/139‑1 (MQ=255)          aCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  1:927419/139‑1 (MQ=255)          aCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   >  1:1393863/1‑139 (MQ=255)          aCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  1:1289751/139‑1 (MQ=255)           cGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTa                                                                                                                  >  1:1036213/1‑139 (MQ=255)                    ctaTCTTCCCCATATTTTGCAACGTAGTGCTCACTGGCGAGGAGAAGATTCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCTGAACTCGGTATTCTTcaca                                                                                                         <  2:729397/137‑1 (MQ=255)                      aTCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACt                                                                                                       <  2:1393863/139‑1 (MQ=255)                       tCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtg                                                                                                      <  2:57510/139‑1 (MQ=255)                        cTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtgt                                                                                                     >  1:1058360/1‑139 (MQ=255)                               atatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAt                                                                                              <  2:1036213/139‑1 (MQ=255)                                          cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                   <  2:695345/139‑1 (MQ=255)                                            tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   >  2:1117431/1‑105 (MQ=255)                                            tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  1:1117431/105‑1 (MQ=255)                                            tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAt                                                                                                                 <  1:1379337/107‑1 (MQ=255)                                            tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAt                                                                                                                 >  2:1379337/1‑107 (MQ=255)                                            tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCatac                                                                                                        >  2:153393/1‑116 (MQ=255)                                            tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                        <  1:153393/116‑1 (MQ=255)                                               tGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  2:1108457/102‑1 (MQ=255)                                               tGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   >  1:1108457/1‑102 (MQ=255)                                                  gcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTtaatcttattaataa                                                                           <  1:1322005/139‑12 (MQ=255)                                                      tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                         <  2:664136/105‑1 (MQ=255)                                                      tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                         >  1:664136/1‑105 (MQ=255)                                                           tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGc                                                                  >  1:844447/1‑139 (MQ=255)                                                                  gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                         >  2:1125130/1‑93 (MQ=255)                                                                  gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                         <  1:1125130/93‑1 (MQ=255)                                                                  gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                  <  1:376251/116‑1 (MQ=255)                                                                  gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                  >  2:376251/1‑116 (MQ=255)                                                                     gCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTTGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTTATAAGATTAAGCAATAGCATGgagcgag                                                        <  2:1058360/139‑1 (MQ=255)                                                                        gCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   >  2:247560/1‑77 (MQ=255)                                                                        gCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  1:247560/77‑1 (MQ=255)                                                                                      caccacGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTa                                                                                                                  >  1:455692/1‑64 (MQ=255)                                                                                      caccacGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTa                                                                                                                  <  2:455692/64‑1 (MQ=255)                                                                                        ccacGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                  <  1:1017655/94‑1 (MQ=255)                                                                                        ccacGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                  >  2:1017655/1‑94 (MQ=255)                                                                                                          aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   >  2:416286/1‑43 (MQ=255)                                                                                                          aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  1:416286/43‑1 (MQ=255)                                                                                                          aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtg                                                                                                      <  2:806264/56‑1 (MQ=255)                                                                                                          aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtg                                                                                                      >  1:806264/1‑56 (MQ=255)                                                                                                          aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGcc                   >  2:59222/1‑139 (MQ=255)                                                                                                              acTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   >  2:1009383/1‑39 (MQ=255)                                                                                                              acTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  1:1009383/39‑1 (MQ=255)                                                                                                              acTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCAt                                               >  1:1040529/1‑107 (MQ=255)                                                                                                              acTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCAt                                               <  2:1040529/107‑1 (MQ=255)                                                                                                                tGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                                <  1:798565/56‑1 (MQ=255)                                                                                                                tGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                                >  2:798565/1‑56 (MQ=255)                                                                                                                tGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGaa             >  2:414862/1‑139 (MQ=255)                                                                                                                 gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  2:909967/36‑1 (MQ=255)                                                                                                                 gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   >  1:909967/1‑36 (MQ=255)                                                                                                                      ggATTGCGTTTTAACGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAATGCCTTCGAACGCGcc       <  1:295618/139‑1 (MQ=255)                                                                                                                       gATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCt      >  2:1415273/1‑139 (MQ=255)                                                                                                                        aTTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCtt     <  1:414862/139‑1 (MQ=255)                                                                                                                        aTTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCtt     <  1:1415273/139‑1 (MQ=255)                                                                                                                          tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                       <  2:1373553/71‑1 (MQ=255)                                                                                                                          tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                       >  1:1373553/1‑71 (MQ=255)                                                                                                                           gCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCgg  <  2:1093820/139‑1 (MQ=255)                                                                                                                            cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCttt                                                                                        <  2:387342/52‑1 (MQ=255)                                                                                                                            cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCttt                                                                                        >  1:387342/1‑52 (MQ=255)                                                                                                                            cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                  >  2:933814/1‑58 (MQ=255)                                                                                                                            cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                   >  1:1162067/1‑57 (MQ=255)                                                                                                                            cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                   <  2:1162067/57‑1 (MQ=255)                                                                                                                            cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGa                        >  2:411595/1‑116 (MQ=255)                                                                                                                            cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGa                        <  1:411595/116‑1 (MQ=255)                                                                                                                            cGTTTTAAAGTTTGCAGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                  <  1:933814/58‑1 (MQ=255)                                                                                                                                   |                                                                                                                                   CCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGG  >  NC_000913/4024206‑4024466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑