| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,279,849 | A→G | intergenic (+21/‑131) | ghxP → / → yjcE | guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,279,849 | 0 | A | G | 98.1% | 186.6 / ‑4.8 | 53 | intergenic (+21/‑131) | ghxP/yjcE | guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (24/28); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
CGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACG > NC_000913/4279714‑4279978 | cgcgACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg < 1:719126/139‑1 (MQ=255)cgcgACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATACGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg < 2:527628/139‑1 (MQ=255) ttGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGc < 1:698946/139‑1 (MQ=255) tGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc > 1:1159028/1‑139 (MQ=255) tGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc < 2:1410927/139‑1 (MQ=255) tcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg > 2:345838/1‑118 (MQ=255) tcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg < 1:345838/118‑1 (MQ=255) tttCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAttt < 1:731753/139‑1 (MQ=255) tCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCAATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtc > 2:931271/1‑139 (MQ=255) aTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGtt < 2:477211/122‑1 (MQ=255) aTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGtt > 1:477211/1‑122 (MQ=255) tCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTg < 2:278959/137‑1 (MQ=255) tCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTg > 1:278959/1‑137 (MQ=255) ggTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAttt < 1:317604/110‑1 (MQ=255) ggTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAttt > 2:317604/1‑110 (MQ=255) gTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGata > 1:381316/1‑139 (MQ=255) cGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTc < 2:1159028/139‑1 (MQ=255) cGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATgag > 2:688619/1‑139 (MQ=255) ggCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtctc > 2:1230668/1‑94 (MQ=255) ggCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtctc < 1:1230668/94‑1 (MQ=255) gcgcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg < 2:822183/63‑1 (MQ=255) gcgcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg > 1:822183/1‑63 (MQ=255) ggTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca > 2:1082442/1‑94 (MQ=255) ggTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca < 1:1082442/94‑1 (MQ=255) gTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGc < 1:767171/71‑1 (MQ=255) gTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGc > 2:767171/1‑71 (MQ=255) gACCTTCTATGAGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa < 1:931271/139‑1 (MQ=255) aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATga < 2:488353/118‑1 (MQ=255) aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATga > 1:488353/1‑118 (MQ=255) aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCttt > 1:1206473/1‑139 (MQ=255) tGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTtc > 1:792587/1‑139 (MQ=255) gCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTtct < 2:1206473/139‑1 (MQ=255) ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCg > 2:727323/1‑74 (MQ=255) ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCg < 1:727323/74‑1 (MQ=255) ggCTGGGCTATCTAATTTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATc < 2:971206/139‑1 (MQ=255) tCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACg < 2:165826/116‑1 (MQ=255) tCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACg > 1:165826/1‑116 (MQ=255) cTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACAt > 2:779370/1‑139 (MQ=255) tCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCa > 2:1047354/1‑139 (MQ=255) tCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCa > 1:774082/1‑139 (MQ=255) cTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCaa < 1:1047354/139‑1 (MQ=255) tttCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCaaa < 1:688619/139‑1 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt < 2:1271924/88‑1 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt > 1:1271924/1‑88 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa < 1:32053/103‑1 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa > 2:32053/1‑103 (MQ=255) tCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca < 2:1113572/53‑1 (MQ=255) tCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca > 1:1113572/1‑53 (MQ=255) aTTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa > 2:700136/1‑111 (MQ=255) aTTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa < 1:700136/111‑1 (MQ=255) aTTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACg < 2:774082/139‑1 (MQ=255) cTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGa < 1:494416/54‑1 (MQ=255) cTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGa > 2:494416/1‑54 (MQ=255) | CGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACG > NC_000913/4279714‑4279978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |