Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,297,668	C→T	D567N (GAC→AAC)	fdhF ←	formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,297,668	0	C	T	100.0%	197.9 / NA	61	D567N (GAC→AAC)	fdhF	formate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/31);  total (30/31)

ATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCC  >  NC_000913/4297538‑4297797                                                                                                                                   |                                                                                                                                  aTTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGgcg                                                                                                                           <  1:1188383/139‑1 (MQ=255)      tgCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTAcc                                                                                                                      <  2:719818/139‑1 (MQ=255)                ggTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAgg                                                                                                            >  1:1195726/1‑139 (MQ=255)                ggTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAgg                                                                                                            >  1:363548/1‑139 (MQ=255)                  ttCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTg                                                                                                          <  2:788029/139‑1 (MQ=255)                     atcaGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaag                                                                                                       >  1:356444/1‑139 (MQ=255)                      tcaGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaaga                                                                                                      >  2:55337/1‑139 (MQ=255)                      tcaGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaaga                                                                                                      >  1:532387/1‑139 (MQ=255)                      tcaGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaaga                                                                                                      >  1:751216/1‑139 (MQ=255)                       cagccagcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     <  2:1287522/139‑1 (MQ=255)                       cagccagcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGACAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     <  2:844811/139‑1 (MQ=255)                            agcGCCGCCAGTGTCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTgcg                                                                                                >  1:1208436/1‑139 (MQ=255)                            agcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGgcg                                                                                                                           <  1:1009754/112‑1 (MQ=255)                            agcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAATAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGgcg                                                                                                                           >  2:1009754/1‑112 (MQ=255)                               gccgccAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACTGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCa                                                                                             <  2:1025999/139‑1 (MQ=255)                                 cgccAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGa                                                                                           <  2:215539/139‑1 (MQ=255)                                 cgccAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGa                                                                                           <  2:532387/139‑1 (MQ=255)                                         ccGCACAGTTACCGTTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTGCTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCgg                                                                                   >  1:870203/1‑139 (MQ=255)                                                 ttACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCaa                                                                           >  1:665304/1‑139 (MQ=255)                                                    ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                        <  2:870203/139‑1 (MQ=255)                                                    ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                        <  2:665304/139‑1 (MQ=255)                                                         cATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCgggg                                                                                 >  2:83769/1‑125 (MQ=255)                                                         cATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCgggg                                                                                 <  1:83769/125‑1 (MQ=255)                                                            cGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTa                                                                <  1:496988/139‑1 (MQ=255)                                                            cGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTa                                                                <  2:751216/139‑1 (MQ=255)                                                              aaCGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTaaa                                                              <  1:557126/139‑1 (MQ=255)                                                                   cAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGAt                                                         <  2:356444/139‑1 (MQ=255)                                                                        gtagtGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaagaa                                                    <  1:292316/139‑1 (MQ=255)                                                                             gACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                    <  2:716775/102‑1 (MQ=255)                                                                             gACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                    >  1:716775/1‑102 (MQ=255)                                                                             gACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTccc                                               >  2:154285/1‑139 (MQ=255)                                                                                   cTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                         >  1:873892/1‑139 (MQ=255)                                                                                   cTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                         <  2:1195726/139‑1 (MQ=255)                                                                                    ttCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCg                                        <  2:73281/139‑1 (MQ=255)                                                                                    ttCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCg                                        <  2:1208436/139‑1 (MQ=255)                                                                                      cACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                      >  2:452363/1‑139 (MQ=255)                                                                                      cACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTAGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCa                                                                                             >  1:935906/1‑84 (MQ=255)                                                                                      cACGCACCGTTGACAGTACCACCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCa                                                                                             <  2:935906/84‑1 (MQ=255)                                                                                        cGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                      >  1:450981/1‑137 (MQ=255)                                                                                        cGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                      <  2:450981/137‑1 (MQ=255)                                                                                             cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGaa                               >  1:1177252/1‑139 (MQ=255)                                                                                             cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGaa                               <  1:154285/139‑1 (MQ=255)                                                                                             cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGaa                               <  1:452363/139‑1 (MQ=255)                                                                                                      tACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCgg                      >  2:1166533/1‑139 (MQ=255)                                                                                                       aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaag                                                                                                       <  2:413844/57‑1 (MQ=255)                                                                                                       aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaag                                                                                                       >  1:413844/1‑57 (MQ=255)                                                                                                       aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGc                     >  1:969307/1‑139 (MQ=255)                                                                                                       aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGc                     >  2:1329289/1‑139 (MQ=255)                                                                                                         cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCaa                   >  2:1083657/1‑139 (MQ=255)                                                                                                           tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAgg                 >  2:732237/1‑139 (MQ=255)                                                                                                              ggTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAgg                 <  2:1274159/136‑1 (MQ=255)                                                                                                              ggTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAgg                 >  1:1274159/1‑136 (MQ=255)                                                                                                                  cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                         <  1:748325/108‑1 (MQ=255)                                                                                                                  cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                         >  2:748325/1‑108 (MQ=255)                                                                                                                   tcgtcgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTgcgc                                                                                               <  1:974271/53‑1 (MQ=255)                                                                                                                   tcgtcgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTgcgc                                                                                               >  2:974271/1‑53 (MQ=255)                                                                                                                    cgtcgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACtgaat        <  2:873892/139‑1 (MQ=255)                                                                                                                       cgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                    >  1:160786/1‑60 (MQ=255)                                                                                                                       cgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                    <  2:160786/60‑1 (MQ=255)                                                                                                                        ggTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAg    >  1:1417511/1‑139 (MQ=255)                                                                                                                          tGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGcc  >  1:25793/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                  ATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCC  >  NC_000913/4297538‑4297797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑