Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,424,516 C→T *92* (TAG→TAA yjfY ← YhcN family protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,5160CT100.0% 70.8 / NA 23*92* (TAG→TAAyjfYYhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

TGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGA  >  NC_000913/4424396‑4424652
                                                                                                                        |                                                                                                                                        
tGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCcgcg                                                                                                                        <  1:129693/139‑1 (MQ=255)
      aaTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCg                                                                                                                  <  2:603484/139‑1 (MQ=255)
      aaTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCg                                                                                                                  <  1:801188/139‑1 (MQ=255)
                 tCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCcgcg                                                                                                                        <  2:938641/122‑1 (MQ=255)
                 tCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCcgcg                                                                                                                        >  1:938641/1‑122 (MQ=255)
                                     ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                  <  1:867999/139‑1 (MQ=255)
                                          cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                             >  1:723474/1‑139 (MQ=255)
                                             ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                          <  1:1319542/139‑1 (MQ=255)
                                               gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                        <  2:723474/139‑1 (MQ=255)
                                                                             ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGtt                                          >  2:1237196/1‑139 (MQ=255)
                                                                                         tATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTTCGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAAt                              <  2:1011332/139‑1 (MQ=255)
                                                                                              cAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTccc                                                                                              >  2:689256/1‑70 (MQ=255)
                                                                                              cAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTccc                                                                                              <  1:689256/70‑1 (MQ=255)
                                                                                                 tGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttct                                       >  2:1139272/1‑122 (MQ=255)
                                                                                                 tGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttct                                       <  1:1139272/122‑1 (MQ=255)
                                                                                                   tttCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGc                                                                                                        >  2:920788/1‑55 (MQ=255)
                                                                                                   tttCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGc                                                                                                        <  1:920788/55‑1 (MQ=255)
                                                                                                   tttCGTTAGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTTCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCATCATTTAATGCCTGACGTg                    >  2:936204/1‑139 (MQ=255)
                                                                                                               taCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACt                                                                                                 <  2:962593/50‑1 (MQ=38)
                                                                                                               taCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACt                                                                                                 >  1:962593/1‑50 (MQ=38)
                                                                                                               taCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAg        >  1:819568/1‑139 (MQ=255)
                                                                                                               taCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCACATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAg        <  1:936204/139‑1 (MQ=255)
                                                                                                                    ggTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCg   <  1:597463/139‑1 (MQ=255)
                                                                                                                     gtgtTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCa         <  2:66326/132‑1 (MQ=255)
                                                                                                                     gtgtTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa  >  2:634655/1‑139 (MQ=255)
                                                                                                                     gtgtTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa  >  1:8853/1‑139 (MQ=255)
                                                                                                                        |                                                                                                                                        
TGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGA  >  NC_000913/4424396‑4424652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: