Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,425,834	G→A	*105* (TAG→TAA)	priB →	primosomal protein N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,425,834	0	G	A	100.0%	195.5 / NA	61	*105* (TAG→TAA)	priB	primosomal protein N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/32);  total (29/32)

TCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTA  >  NC_000913/4425708‑4425968                                                                                                                               |                                                                                                                                       tCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACg                                                                                                                            <  2:1127549/139‑1 (MQ=255)      gTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTAtt                                                                                                                       >  1:1384309/1‑139 (MQ=255)         taACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTcc                                                                                                                    >  1:1226798/1‑139 (MQ=255)           aCGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcgt                                                                                                                  <  2:1226798/139‑1 (MQ=255)             ggTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcgtcg                                                                                                                >  1:1123239/1‑139 (MQ=255)                 ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                                <  1:1286952/119‑1 (MQ=255)                 ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                                >  2:1286952/1‑119 (MQ=255)                 ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCAc                                                                                                                             >  2:1218696/1‑122 (MQ=255)                 ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCAc                                                                                                                             <  1:1218696/122‑1 (MQ=255)                 ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGc                                                                                                            >  1:408379/1‑139 (MQ=255)                 ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGc                                                                                                            >  2:315479/1‑139 (MQ=255)                 ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGc                                                                                                            >  2:605590/1‑139 (MQ=255)                    aGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAg                                                                                                         <  1:493352/139‑1 (MQ=255)                                        gTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCgtcgtc                                                                                                              >  2:710310/1‑114 (MQ=255)                                        gTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCgtcgtc                                                                                                              <  1:710310/114‑1 (MQ=255)                                        gTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGa                                                                                     <  1:315479/139‑1 (MQ=255)                                        gTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGa                                                                                     <  2:1123239/139‑1 (MQ=255)                                          tcatttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                   <  2:1384309/139‑1 (MQ=255)                                           catttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAgg                                                                                  >  2:666709/1‑139 (MQ=255)                                           catttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAgg                                                                                  >  2:157340/1‑139 (MQ=255)                                                       caAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                                      <  2:282480/139‑1 (MQ=255)                                                        aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGt                                                                                                        >  1:221775/1‑104 (MQ=255)                                                        aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                     <  1:666709/139‑1 (MQ=255)                                                        aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                     >  1:1346067/1‑139 (MQ=255)                                                        aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                     <  1:778515/139‑1 (MQ=255)                                                        aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                     <  1:1217346/139‑1 (MQ=255)                                                        aaGGCAAAGAACGGACTGAGCAAAATGGTTGTTCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGGCGCAAGt                                                                                                        <  2:221775/104‑1 (MQ=255)                                                              aaGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACtat                                                               <  1:50634/139‑1 (MQ=255)                                                              aaGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACtat                                                               <  2:1346067/139‑1 (MQ=255)                                                               aGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACtata                                                              <  1:605590/139‑1 (MQ=255)                                                                     gACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGata                                                        >  1:254418/1‑139 (MQ=255)                                                                         gAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGc                                                    >  2:2359/1‑139 (MQ=255)                                                                          aGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGa                                                                                     <  1:1057038/105‑1 (MQ=255)                                                                          aGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGa                                                                                     >  2:1057038/1‑105 (MQ=255)                                                                           gCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                   >  2:1386951/1‑106 (MQ=255)                                                                           gCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                   <  1:1386951/106‑1 (MQ=255)                                                                                    ttttGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCg                                                     >  2:875168/1‑127 (MQ=255)                                                                                    ttttGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCg                                                     <  1:875168/127‑1 (MQ=255)                                                                                     tttGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaaaa                                        <  1:2359/139‑1 (MQ=255)                                                                                                aGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcg                                                                                                                   <  2:1294219/53‑1 (MQ=255)                                                                                                aGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcg                                                                                                                   >  1:1294219/1‑53 (MQ=255)                                                                                                   aGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaaa                          <  2:516987/139‑1 (MQ=255)                                                                                                      ttgaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                     <  2:995558/93‑1 (MQ=255)                                                                                                      ttgaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                     >  1:995558/1‑93 (MQ=255)                                                                                                        gaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGt                     >  1:115500/1‑139 (MQ=255)                                                                                                          attgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCg                                                                    <  2:195666/90‑1 (MQ=255)                                                                                                          attgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCg                                                                    >  1:195666/1‑90 (MQ=255)                                                                                                          attgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                                   <  2:186590/91‑1 (MQ=255)                                                                                                          attgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                                   >  1:186590/1‑91 (MQ=255)                                                                                                              aTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                                   <  1:671046/87‑1 (MQ=255)                                                                                                              aTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                                   >  2:671046/1‑87 (MQ=255)                                                                                                               tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGttt                                                                                              >  1:794555/1‑59 (MQ=255)                                                                                                               tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGttt                                                                                              <  2:794555/59‑1 (MQ=255)                                                                                                               tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACtata                                                              >  2:377570/1‑91 (MQ=255)                                                                                                               tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACtata                                                              <  1:377570/91‑1 (MQ=255)                                                                                                               tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTg              >  1:242267/1‑139 (MQ=255)                                                                                                                       ggAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGc      <  1:157340/139‑1 (MQ=255)                                                                                                                        gagaCTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGcc     >  2:1207434/1‑139 (MQ=255)                                                                                                                            cTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAATACTACATCACCGAAAg                         <  2:647438/115‑1 (MQ=255)                                                                                                                            cTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAATACTACATCACCGAAAg                         >  1:647438/1‑115 (MQ=255)                                                                                                                            cTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTa  <  2:965274/138‑1 (MQ=255)                                                                                                                               |                                                                                                                                       TCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTA  >  NC_000913/4425708‑4425968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑