Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,447,891 G→A *1260* (TAG→TAA tamB → translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,447,8910GA100.0% 280.5 / NA 86*1260* (TAG→TAAtamBtranslocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/39);  total (47/39)

TGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCG  >  NC_000913/4447758‑4448023
                                                                                                                                     |                                                                                                                                    
tGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGc                                                                                                                                <  2:1371456/139‑1 (MQ=255)
            tGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCt                                                                                                                    >  1:1072332/1‑139 (MQ=255)
              ggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                                                  <  1:1111259/123‑1 (MQ=255)
              ggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                                                  >  2:1111259/1‑123 (MQ=255)
                      tGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGttt                                                                                                          <  2:1072332/139‑1 (MQ=255)
                        aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                                        >  1:1116374/1‑139 (MQ=255)
                         ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                                          >  2:1236673/1‑120 (MQ=255)
                         ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                                          <  1:1236673/120‑1 (MQ=255)
                         ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                                       >  1:1110847/1‑139 (MQ=255)
                         ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                                       >  2:225896/1‑139 (MQ=255)
                         ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                                       >  2:155711/1‑139 (MQ=255)
                                    cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                                            >  1:393252/1‑139 (MQ=255)
                                    cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                                            >  1:1428142/1‑139 (MQ=255)
                                      cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCGCTATCAGTTCGAGTTTTGACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                          >  2:919587/1‑139 (MQ=255)
                                        cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  2:1428142/139‑1 (MQ=255)
                                        cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  2:393252/139‑1 (MQ=255)
                                        cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  2:1116374/139‑1 (MQ=255)
                                          cgttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                                                  >  1:1266209/1‑95 (MQ=255)
                                          cgttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                                                  <  2:1266209/95‑1 (MQ=255)
                                          cgttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACa                                                                                      >  2:1119828/1‑139 (MQ=255)
                                           gttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAg                                                                                     >  2:51408/1‑139 (MQ=255)
                                                     cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                                           >  2:721678/1‑139 (MQ=255)
                                                     cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                                           >  1:984218/1‑139 (MQ=255)
                                                          gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                      <  2:984218/139‑1 (MQ=255)
                                                          gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                      <  1:51408/139‑1 (MQ=255)
                                                                tAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATg                                                                    <  2:832992/135‑1 (MQ=255)
                                                                tAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATg                                                                    >  1:832992/1‑135 (MQ=255)
                                                                tAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCa                                                                >  2:197648/1‑139 (MQ=255)
                                                                       tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                  >  2:1119527/1‑130 (MQ=255)
                                                                       tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                  <  1:1119527/130‑1 (MQ=255)
                                                                         tCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTggg                                                       <  1:155711/139‑1 (MQ=255)
                                                                          cTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      <  1:438945/139‑1 (MQ=255)
                                                                             gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGttt                                                                                                          >  2:641187/1‑84 (MQ=255)
                                                                             gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGttt                                                                                                          <  1:641187/84‑1 (MQ=255)
                                                                             gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                                         >  1:612941/1‑85 (MQ=255)
                                                                             gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                                         <  2:612941/85‑1 (MQ=255)
                                                                             gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      >  1:791322/1‑136 (MQ=255)
                                                                             gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      <  2:791322/136‑1 (MQ=255)
                                                                              aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAtt                                                  >  2:313379/1‑139 (MQ=255)
                                                                                 ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGa                                                                             <  1:623406/109‑1 (MQ=255)
                                                                                 ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGa                                                                             >  2:623406/1‑109 (MQ=255)
                                                                                  cGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGa                                                                             >  2:558573/1‑108 (MQ=255)
                                                                                  cGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGa                                                                             <  1:558573/108‑1 (MQ=255)
                                                                                  cGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAg                                              >  1:23277/1‑139 (MQ=255)
                                                                                    tgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                                                     <  2:93735/82‑1 (MQ=255)
                                                                                    tgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                                                     >  1:93735/1‑82 (MQ=255)
                                                                                     gtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                                           >  1:736538/1‑139 (MQ=255)
                                                                                        tGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa                                        <  1:128058/139‑1 (MQ=255)
                                                                                          gtgtAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                                      <  2:1110847/139‑1 (MQ=255)
                                                                                                aCCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                                                       >  2:805295/1‑100 (MQ=255)
                                                                                                aCCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                                                       <  1:805295/100‑1 (MQ=255)
                                                                                                  cAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                              <  2:736538/139‑1 (MQ=255)
                                                                                                    ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                      <  1:1144718/97‑1 (MQ=255)
                                                                                                    ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                      >  2:1144718/1‑97 (MQ=255)
                                                                                                    ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATg                                                                    <  2:407302/99‑1 (MQ=255)
                                                                                                    ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATg                                                                    >  1:407302/1‑99 (MQ=255)
                                                                                                     gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                           >  2:12857/1‑139 (MQ=255)
                                                                                                     gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGACAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTAc                                                           >  2:970800/1‑107 (MQ=255)
                                                                                                     gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGACAGTTTACGCCACAAACAAGGCAACAGTCACTAGATGACCAATGCCCAGCTAc                                                           <  1:970800/107‑1 (MQ=255)
                                                                                                          tggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGtata                       >  2:1187303/5‑139 (MQ=255)
                                                                                                           ggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                  >  1:1043641/4‑95 (MQ=255)
                                                                                                           ggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                  <  2:1043641/92‑1 (MQ=255)
                                                                                                           ggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAg                      >  2:25658/4‑139 (MQ=255)
                                                                                                           ggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAg                      >  1:387653/4‑139 (MQ=255)
                                                                                                                 ctctATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      >  1:1059385/1‑101 (MQ=255)
                                                                                                                 ctctATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      <  2:1059385/101‑1 (MQ=255)
                                                                                                                    tATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                                                    >  2:187522/1‑68 (MQ=255)
                                                                                                                    tATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                                                    <  1:187522/68‑1 (MQ=255)
                                                                                                                      tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      >  2:356528/1‑96 (MQ=255)
                                                                                                                      tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      <  1:356528/96‑1 (MQ=255)
                                                                                                                      tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACt           >  2:95613/1‑139 (MQ=255)
                                                                                                                      tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACt           >  2:676850/1‑139 (MQ=255)
                                                                                                                      tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACt           >  1:1417426/1‑139 (MQ=255)
                                                                                                                           tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                      >  2:300558/1‑75 (MQ=255)
                                                                                                                           tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                      <  1:300558/75‑1 (MQ=255)
                                                                                                                           tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                 >  2:558292/1‑112 (MQ=255)
                                                                                                                           tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                 <  1:558292/112‑1 (MQ=255)
                                                                                                                           tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                  >  1:1202742/1‑127 (MQ=255)
                                                                                                                           tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                  <  2:1202742/127‑1 (MQ=255)
                                                                                                                           tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCa      >  2:814925/1‑139 (MQ=255)
                                                                                                                             gAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAgg    <  1:1119828/139‑1 (MQ=255)
                                                                                                                              aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      >  2:722737/1‑88 (MQ=255)
                                                                                                                              aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      <  1:722737/88‑1 (MQ=255)
                                                                                                                               gTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgcg  <  2:23277/139‑1 (MQ=255)
                                                                                                                               gTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgcg  <  2:1417426/139‑1 (MQ=255)
                                                                                                                               gTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgcg  <  1:313379/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCG  >  NC_000913/4447758‑4448023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: