Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,460,932	A→G	F577L (TTC→CTC)	nrdD ←	anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,460,932	0	A	G	100.0%	166.8 / NA	48	F577L (TTC→CTC)	nrdD	anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)

CAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTTTCTTCT  >  NC_000913/4460798‑4461065                                                                                                                                       |                                                                                                                                      cAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTg                                                                                                                                   <  1:930585/139‑1 (MQ=255)    cgcCTTCAGGTTGTGCTGAATGTTTGGATACTAGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTg                                                                                                                                >  2:848959/1‑139 (MQ=255)        ttCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGgtgt                                                                                                                            >  2:788742/1‑139 (MQ=255)                              gATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGcc                                                                                                      <  2:1373491/139‑1 (MQ=255)                                    cGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGcac                                                                                                <  1:354203/139‑1 (MQ=255)                                     gCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGcacc                                                                                               >  2:1038858/1‑139 (MQ=255)                                       cGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGAGGAGACCGTTGGTGTAGTAACCTTTATCGGTTAACGCCGGcaccac                                                                                             >  2:1190598/1‑139 (MQ=255)                                        gTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACg                                                                                            <  2:485121/139‑1 (MQ=255)                                             aaaTGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCaaa                                                                                       <  2:1391871/139‑1 (MQ=255)                                             aaaTGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCaaa                                                                                       <  1:635812/139‑1 (MQ=255)                                                 gAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTca                                                                                   >  1:381115/1‑139 (MQ=255)                                                  aaaCCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATTGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTcag                                                                                  <  1:848959/139‑1 (MQ=255)                                                  aaaCCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTcag                                                                                  <  1:212275/139‑1 (MQ=255)                                                           ttCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCga                                                                         <  1:1038858/139‑1 (MQ=255)                                                             cGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTg                                                                                                                                >  2:439085/1‑82 (MQ=255)                                                             cGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCgaga                                                                       <  2:407593/139‑1 (MQ=255)                                                             cGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACAACGAGGTGGAGACTGTTg                                                                                                                                <  1:439085/82‑1 (MQ=255)                                                                cAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTgtag                                                                                                                          <  2:135241/85‑1 (MQ=255)                                                                cAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTgtag                                                                                                                          >  1:135241/1‑85 (MQ=255)                                                                      cggGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAg                                                              <  2:381115/139‑1 (MQ=255)                                                                        ggTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGcc                                                                                          >  2:118026/1‑109 (MQ=255)                                                                        ggTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGcc                                                                                          <  1:118026/109‑1 (MQ=255)                                                                              gcgcTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTTGAGACTGTTGGTGTACTATCCTTTATCGGTCACGCCCGCCACCACGCCAAAGTCAGCAGTATCGAGACGGCAGAAGCGATCGca                                                      <  2:1016062/139‑1 (MQ=255)                                                                              gcgcTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGca                                                      <  1:57797/139‑1 (MQ=255)                                                                              gcgcTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGca                                                      <  1:1190598/139‑1 (MQ=255)                                                                                   tCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGTAGTATCGAGACGGCAGAAGCGATCGCACAGGt                                                 >  2:10415/1‑139 (MQ=255)                                                                                                   cGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCa                                                                  >  1:501863/1‑106 (MQ=255)                                                                                                   cGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCa                                                                  <  2:501863/106‑1 (MQ=255)                                                                                                       cGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCgaga                                                                       >  2:23320/1‑97 (MQ=255)                                                                                                       cGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCgaga                                                                       <  1:23320/97‑1 (MQ=255)                                                                                                       cGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTag                             >  1:746908/1‑139 (MQ=255)                                                                                                           ttCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGTAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGAc                         >  2:1000786/1‑139 (MQ=255)                                                                                                             cACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTg                       >  1:810405/1‑139 (MQ=255)                                                                                                                    ttctCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCg                                        >  1:380607/1‑115 (MQ=255)                                                                                                                    ttctCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCg                                        <  2:380607/115‑1 (MQ=255)                                                                                                                     tctcCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGACAGAAGCGATCGcaca                                                    <  1:105495/102‑1 (MQ=255)                                                                                                                     tctcCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGACAGAAGCGATCGcaca                                                    >  2:105495/1‑102 (MQ=255)                                                                                                                        ccACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCaaa                                                                                       <  1:524991/64‑1 (MQ=255)                                                                                                                        ccACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCaaa                                                                                       >  2:524991/1‑64 (MQ=255)                                                                                                                          acaTCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTc                                                                                                           <  1:1168975/42‑1 (MQ=255)                                                                                                                          acaTCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTc                                                                                                           >  2:1168975/1‑42 (MQ=255)                                                                                                                           caTCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGt         <  2:746908/139‑1 (MQ=255)                                                                                                                              cGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttc      >  2:107451/1‑139 (MQ=255)                                                                                                                              cGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttc      <  1:107451/139‑1 (MQ=255)                                                                                                                              cGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAACAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATATCCCGTttc      >  1:849691/1‑139 (MQ=255)                                                                                                                                aGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttctt    >  1:96672/1‑139 (MQ=255)                                                                                                                                aGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttctt    >  2:327592/1‑139 (MQ=255)                                                                                                                                 ggTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttcttc   >  1:11275/1‑139 (MQ=255)                                                                                                                                  gTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTTtcttct  <  2:11275/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTTTCTTCT  >  NC_000913/4460798‑4461065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑