Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,638,120 G→A *475* (TAG→TAA creC → sensory histidine kinase in two‑component regulatory system with CreB or PhoB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,638,1200GA100.0% 124.2 / NA 40*475* (TAG→TAAcreCsensory histidine kinase in two‑component regulatory system with CreB or PhoB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/23);  total (17/23)

AAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATG  >  NC_000913/4637994‑4638252
                                                                                                                              |                                                                                                                                    
aaGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAAttctt                                                                                                                          <  1:483479/139‑1 (MQ=255)
    agcGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCca                                                                                                                      <  1:695012/139‑1 (MQ=255)
       ggTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACAt                                                                                                                   <  2:471053/139‑1 (MQ=255)
       ggTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACAt                                                                                                                   <  1:894121/139‑1 (MQ=255)
        gTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATa                                                                                                                  >  2:1016516/1‑139 (MQ=255)
              ggTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTc                                                                                                            <  2:452796/139‑1 (MQ=255)
                   gCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATc                                                                                                       >  1:858466/1‑139 (MQ=255)
                                 ggTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCa                                                                                         <  1:424423/139‑1 (MQ=255)
                                  gTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCaa                                                                                        <  1:1016516/139‑1 (MQ=255)
                                     gCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCcaca                                                                                                                    >  1:625096/1‑108 (MQ=255)
                                     gCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCcaca                                                                                                                    <  2:625096/108‑1 (MQ=255)
                                         gtttgtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGagaa                                                                                 <  1:312467/139‑1 (MQ=255)
                                          tttgtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTcc                                                                                                                        >  2:79531/1‑99 (MQ=255)
                                          tttgtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTcc                                                                                                                        <  1:79531/99‑1 (MQ=255)
                                           ttgtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGagaaga                                                                               <  1:402025/139‑1 (MQ=255)
                                             gtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACt                                                                             <  2:713899/139‑1 (MQ=255)
                                                    ggCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCcaca                                                                                                                    >  2:706245/1‑93 (MQ=255)
                                                    ggCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCcaca                                                                                                                    <  1:706245/93‑1 (MQ=255)
                                                             aCGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATcccccc                                                             >  2:862308/1‑139 (MQ=255)
                                                                        cGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCa                                                                                         >  2:1024879/1‑100 (MQ=255)
                                                                        cGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCa                                                                                         <  1:1024879/100‑1 (MQ=255)
                                                                                    tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAAttc                                                                                                                            <  1:311462/53‑1 (MQ=255)
                                                                                    tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAAttc                                                                                                                            >  2:311462/1‑53 (MQ=255)
                                                                                    tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTg                                      >  1:1061535/1‑139 (MQ=255)
                                                                                    tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACACAg                                                                                                                 <  2:967363/64‑1 (MQ=255)
                                                                                    tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTATTCCCACATAg                                                                                                                 >  1:967363/1‑64 (MQ=255)
                                                                                      gCGTGCTGTCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                                    <  1:353848/139‑1 (MQ=255)
                                                                                       cGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAg                                                                                                                 <  2:1129960/61‑1 (MQ=255)
                                                                                       cGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAg                                                                                                                 >  1:1129960/1‑61 (MQ=255)
                                                                                          gCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGga                                                                                    <  2:597502/87‑1 (MQ=255)
                                                                                          gCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGga                                                                                    >  1:597502/1‑87 (MQ=255)
                                                                                           cTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTg                               <  2:262078/139‑1 (MQ=255)
                                                                                             ggCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAAt                                                                                                                              >  2:1253238/1‑42 (MQ=255)
                                                                                             ggCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAAt                                                                                                                              <  1:1253238/42‑1 (MQ=255)
                                                                                                             cACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGa             >  1:375662/1‑139 (MQ=255)
                                                                                                              aCCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGAt            >  2:1340193/1‑139 (MQ=255)
                                                                                                              aCCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGAt            <  2:858466/139‑1 (MQ=255)
                                                                                                                  tCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGagaaga                                                                               <  1:798629/68‑1 (MQ=255)
                                                                                                                  tCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGagaaga                                                                               >  2:798629/1‑68 (MQ=255)
                                                                                                                        cacaTAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATg  >  2:1279536/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                    
AAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATG  >  NC_000913/4637994‑4638252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: