Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	385,737	A→G	D169G (GAT→GGT)	tauA →	taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	385,737	0	A	G	100.0%	144.5 / NA	40	D169G (GAT→GGT)	tauA	taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/20);  total (20/20)

CGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAA  >  NC_000913/385606‑385864                                                                                                                                    |                                                                                                                                cGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGAt                                                                                                                          >  2:1074730/1‑139 (MQ=255)  gCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATg                                                                                                                         <  1:131545/139‑1 (MQ=255)           tttATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATg                                                                                                                >  1:1226591/1‑139 (MQ=255)           tttATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATg                                                                                                                >  2:855189/1‑139 (MQ=255)                 tcCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTggg                                                                                                          <  2:1123004/139‑1 (MQ=255)                  ccaccaCCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGc                                                                                                         >  1:1147835/1‑139 (MQ=255)                           acTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGt                                                                                                >  2:1003187/1‑139 (MQ=255)                             tACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                              <  2:328667/139‑1 (MQ=255)                              aCAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTaa                                                                                             <  1:855189/139‑1 (MQ=255)                              aCAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTaa                                                                                             <  2:1226591/139‑1 (MQ=255)                                  ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcg                                                                                                     >  1:1014130/1‑127 (MQ=255)                                  ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcg                                                                                                     <  2:1014130/127‑1 (MQ=255)                                       tgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGa                                                                                    >  1:1419535/1‑139 (MQ=255)                                          cggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaa                                                                                 <  2:1147835/139‑1 (MQ=255)                                           ggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaaa                                                                                >  2:1341209/1‑139 (MQ=255)                                            gcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaa                                                                                 <  1:375111/137‑1 (MQ=255)                                            gcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaa                                                                                 >  2:375111/1‑137 (MQ=255)                                                  aaaCACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                         <  2:212776/139‑1 (MQ=255)                                                            gCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                               <  1:1074730/139‑1 (MQ=255)                                                            gCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                               <  1:1341209/139‑1 (MQ=255)                                                             cATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTc                                                                                                              <  1:656987/91‑1 (MQ=255)                                                             cATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTc                                                                                                              >  2:656987/1‑91 (MQ=255)                                                                     ccGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGa                                                      <  1:484336/139‑1 (MQ=255)                                                                      cGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGaa                                                     >  2:192490/1‑139 (MQ=255)                                                                                       tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTgggg                                    >  2:201154/1‑139 (MQ=255)                                                                                        gAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGgc                                   >  2:1217379/1‑139 (MQ=255)                                                                                         aaCCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGAt                                                                                                                          >  2:239894/1‑51 (MQ=255)                                                                                         aaCCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGAt                                                                                                                          <  1:239894/51‑1 (MQ=255)                                                                                           ccTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACg                                                                                           <  1:567605/80‑1 (MQ=255)                                                                                           ccTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACg                                                                                           >  2:567605/1‑80 (MQ=255)                                                                                               cAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGgtat                                                                                                                              <  2:952613/41‑1 (MQ=255)                                                                                               cAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGgtat                                                                                                                              >  1:952613/1‑41 (MQ=255)                                                                                                      ccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGa                     <  2:1419535/139‑1 (MQ=255)                                                                                                                    gCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                         <  2:882398/73‑1 (MQ=255)                                                                                                                    gCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                         >  1:882398/1‑73 (MQ=255)                                                                                                                      cTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                               <  2:180709/81‑1 (MQ=255)                                                                                                                      cTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                               >  1:180709/1‑81 (MQ=255)                                                                                                                         gCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcgg                                                                                                    <  1:166170/41‑1 (MQ=255)                                                                                                                         gCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcgg                                                                                                    >  2:166170/1‑41 (MQ=255)                                                                                                                         gCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCaaa  >  2:17893/1‑139 (MQ=255)                                                                                                                          cAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTg                       >  2:501036/1‑117 (MQ=255)                                                                                                                          cAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTg                       <  1:501036/117‑1 (MQ=255)                                                                                                                                    |                                                                                                                                CGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAA  >  NC_000913/385606‑385864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑