Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	414,875	A→G	R293R (CGT→CGC)	sbcC ←	exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	414,875	0	A	G	100.0%	149.3 / NA	42	R293R (CGT→CGC)	sbcC	exonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

GACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAAC  >  NC_000913/414741‑415010                                                                                                                                       |                                                                                                                                        gACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGaa                                                                                                                                     >  2:1078829/1‑139 (MQ=255)         cgccgcGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGt                                                                                                                             <  1:1084983/139‑1 (MQ=255)          gccgcGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTg                                                                                                                            <  1:932187/139‑1 (MQ=255)              cgTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                        <  1:739348/139‑1 (MQ=255)                            cTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTg                                                                                                                            >  2:218104/1‑121 (MQ=255)                            cTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTg                                                                                                                            <  1:218104/121‑1 (MQ=255)                              cgcgcgAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAgc                                                                                                        <  1:1117535/139‑1 (MQ=255)                                                 ctctGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTTCCgg                                                                                                                        >  1:715669/1‑104 (MQ=255)                                                 ctctGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                        <  2:715669/104‑1 (MQ=255)                                                              tATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTACCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTatcttcttc                                                                        >  1:849699/1‑139 (MQ=255)                                                                       cttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGc                                                               <  1:1078829/139‑1 (MQ=255)                                                                        ttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGcgccg                                                                                                    <  1:1093018/101‑1 (MQ=255)                                                                        ttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGcgccg                                                                                                    >  2:1093018/1‑101 (MQ=255)                                                                        ttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGcc                                                              <  1:821785/139‑1 (MQ=255)                                                                        ttcAATCTGCTGGCGAATATGCACCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGtt                                                                                                                      <  2:548174/83‑1 (MQ=255)                                                                        ttcAATCTGCTGGCGAATATGCACCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGtt                                                                                                                      >  1:548174/1‑83 (MQ=255)                                                                         tcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                        >  2:1287195/1‑80 (MQ=255)                                                                         tcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                        <  1:1287195/80‑1 (MQ=255)                                                                         tcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTgc                                                                                      <  1:482750/114‑1 (MQ=255)                                                                         tcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTgc                                                                                      >  2:482750/1‑114 (MQ=255)                                                                              ctgctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTCGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGc                                                        <  2:849699/139‑1 (MQ=255)                                                                                 ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                        >  2:1173532/1‑72 (MQ=255)                                                                                 ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                        <  1:1173532/72‑1 (MQ=255)                                                                                 ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTgc                                                                                      <  2:85530/106‑1 (MQ=255)                                                                                 ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTgc                                                                                      >  1:85530/1‑106 (MQ=255)                                                                                 ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAg                                                     >  2:290714/1‑139 (MQ=255)                                                                                  tgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAgg                                                    >  2:561805/1‑139 (MQ=255)                                                                                              gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTaa                                                                  >  2:1005129/1‑113 (MQ=255)                                                                                              gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTaa                                                                  <  1:1005129/113‑1 (MQ=255)                                                                                              gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCaa                                                      >  1:1308669/1‑125 (MQ=255)                                                                                              gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCaa                                                      <  2:1308669/125‑1 (MQ=255)                                                                                              gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcgg                                         <  1:725669/138‑1 (MQ=255)                                                                                              gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcgg                                         >  2:725669/1‑138 (MQ=255)                                                                                                cAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcggcgg                                      >  2:913790/1‑139 (MQ=255)                                                                                                     ccgcGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCttttt                                                                               <  2:976790/93‑1 (MQ=255)                                                                                                     ccgcGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCttttt                                                                               >  1:976790/1‑93 (MQ=255)                                                                                                                       tGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTc               >  2:35019/1‑139 (MQ=255)                                                                                                                         cGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCt             >  1:1009308/1‑139 (MQ=255)                                                                                                                              ccAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGcttc                              <  2:1123577/117‑1 (MQ=255)                                                                                                                              ccAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGcttc                              >  1:1123577/1‑117 (MQ=255)                                                                                                                                 gtgtGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGtt     <  1:561805/139‑1 (MQ=255)                                                                                                                                    tgGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAAc  <  1:290714/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAAC  >  NC_000913/414741‑415010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑