Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	444,088	C→T	R143Q (CGG→CAG)	panE ←	2‑dehydropantoate reductase, NADPH‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	444,088	0	C	T	100.0%	202.1 / NA	63	R143Q (CGG→CAG)	panE	2‑dehydropantoate reductase, NADPH‑specific
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/31);  total (32/31)

AGTCAGTGGATTAATCACGCAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCCGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGT  >  NC_000913/443955‑444223                                                                                                                                      |                                                                                                                                        agtcagtGGATTAATCACGCAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTtgctgtgcc                                                                                                                                    <  1:535125/139‑1 (MQ=255) agtcagtGGATTAATCACGCAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTtgctgtgcc                                                                                                                                    <  2:582398/139‑1 (MQ=255) agtcagtGGATTAATCACGCAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTtgctgtgcc                                                                                                                                    >  2:680018/1‑139 (MQ=255)     agtGGATTAATCACGCAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCggac                                                                                                                                <  2:1061399/139‑3 (MQ=255)         gATTAATCACGCAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCAAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAat                                                                                                                            <  1:606433/139‑1 (MQ=255)              aTCACGCAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCg                                                                                                                       >  2:645373/1‑139 (MQ=255)                 aCGCAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGa                                                                                                                    >  1:1168864/1‑139 (MQ=255)                    cAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGc                                                                                                                                <  2:265962/124‑1 (MQ=255)                    cAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGc                                                                                                                                >  1:265962/1‑124 (MQ=255)                    cAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATAc                                                                                                                 >  1:1063175/1‑139 (MQ=255)                    cAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATAc                                                                                                                 <  1:1191168/139‑1 (MQ=255)                       ttGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGt                                                                                                              >  1:119000/1‑139 (MQ=255)                        tGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGtt                                                                                                             >  2:1071796/1‑139 (MQ=255)                               cAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCcgca                                                                                                      <  2:1168864/139‑1 (MQ=255)                               cAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCcaca                                                                                                      <  1:1301227/139‑1 (MQ=255)                               cAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCcaca                                                                                                      <  1:1071796/139‑1 (MQ=255)                                           caGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCgg                                                                                                                                  >  1:1319975/1‑99 (MQ=255)                                           caGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCgg                                                                                                                                  <  2:1319975/99‑1 (MQ=255)                                                  gcgcGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAAAGACATTGCCGt                                                                                   <  2:422552/139‑1 (MQ=255)                                                                 tGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCcaca                                                                                                      >  2:638564/1‑105 (MQ=255)                                                                 tGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCcaca                                                                                                      <  1:638564/105‑1 (MQ=255)                                                                 tGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCAt                                                                    >  2:1032186/1‑139 (MQ=255)                                                                 tGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCAt                                                                    >  1:43012/1‑139 (MQ=255)                                                                 tGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCAt                                                                    <  1:680018/139‑1 (MQ=255)                                                                  gCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATg                                                                   >  1:469742/1‑139 (MQ=255)                                                                      ggCAACGTCAGGCAACACGGTTTGCAAAATATCCGTCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAat                                                                                                                            <  1:934213/78‑1 (MQ=255)                                                                      ggCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCGATATGCGTGATACCGTGTGCcaca                                                                                                      <  1:873812/100‑1 (MQ=255)                                                                      ggCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAat                                                                                                                            >  2:934213/1‑78 (MQ=255)                                                                      ggCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCcaca                                                                                                      <  1:705728/100‑1 (MQ=255)                                                                      ggCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCcaca                                                                                                      >  2:705728/1‑100 (MQ=255)                                                                      ggCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCcaca                                                                                                      >  2:873812/1‑100 (MQ=255)                                                                      ggCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACAt                                                                                                     >  2:329341/1‑101 (MQ=255)                                                                      ggCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACAt                                                                                                     <  1:329341/101‑1 (MQ=255)                                                                       gCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGggtgg                                                              <  2:119000/139‑1 (MQ=255)                                                                        cAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATa                                                                                                                  <  1:1312478/86‑1 (MQ=255)                                                                        cAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATa                                                                                                                  >  2:1312478/1‑86 (MQ=255)                                                                            gTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGgtggtggtg                                                         >  1:1274302/1‑139 (MQ=255)                                                                               aGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGccc                                                      <  2:1274302/139‑1 (MQ=255)                                                                                ggCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCa                                                     <  2:43012/139‑1 (MQ=255)                                                                                   aaCACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGgtggtggtg                                                         <  2:1036319/132‑1 (MQ=255)                                                                                   aaCACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGgtggtggtg                                                         >  1:1036319/1‑132 (MQ=255)                                                                                   aaCACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCa                                                  >  1:332746/1‑139 (MQ=255)                                                                                     cacGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAatat                                                                                                                          >  2:993044/1‑65 (MQ=255)                                                                                     cacGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAatat                                                                                                                          <  1:993044/65‑1 (MQ=255)                                                                                      acGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTa                                               >  2:327233/1‑139 (MQ=255)                                                                                           ttGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGgc                                          >  2:753966/1‑139 (MQ=255)                                                                                           ttGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGgc                                          >  1:153847/1‑139 (MQ=255)                                                                                            tGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGcc                                                                                                         <  2:292272/75‑1 (MQ=255)                                                                                            tGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGcc                                                                                                         >  1:292272/1‑75 (MQ=255)                                                                                                  atatCCGCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCGAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGaa                                   <  1:327233/139‑1 (MQ=255)                                                                                                        gCCAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGtttt                             >  2:739332/1‑139 (MQ=255)                                                                                                          cAGATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGc                           <  1:645373/139‑1 (MQ=255)                                                                                                            gATAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGCaa                         <  2:469742/139‑1 (MQ=255)                                                                                                             aTAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGccc                                                      <  1:900311/109‑1 (MQ=255)                                                                                                             aTAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGCAAc                        >  2:1163661/1‑139 (MQ=255)                                                                                                             aTAACTGTAATCCCCGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGCGGTGGTGccc                                                      >  2:900311/1‑109 (MQ=255)                                                                                                                        ccccGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTcgcg                                                                               >  2:564641/1‑73 (MQ=255)                                                                                                                        ccccGTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTcgcg                                                                               <  1:564641/73‑1 (MQ=255)                                                                                                                            gTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGCAACTCTTCGATGGTGccc         >  1:359183/1‑139 (MQ=255)                                                                                                                            gTCCTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGCAACTCTTCGATGGTGccc         >  1:108976/1‑139 (MQ=255)                                                                                                                               cTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGCAACTCTTCGATGGTGCCCATg      <  2:359183/139‑1 (MQ=255)                                                                                                                               cTGTTGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGCAACTCTTCGATGGTGCCCATg      <  1:1032186/139‑1 (MQ=255)                                                                                                                                  ttGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCg                                                                                    <  1:1009450/58‑1 (MQ=255)                                                                                                                                  ttGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGgctgc                                       >  2:1239015/1‑103 (MQ=255)                                                                                                                                  ttGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGgctgc                                       <  1:1239015/103‑1 (MQ=255)                                                                                                                                  ttGCTGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAACAATGACATTGCCg                                                                                    >  2:1009450/1‑58 (MQ=255)                                                                                                                                   tgctgtgccgGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGt  <  1:739332/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        AGTCAGTGGATTAATCACGCAGTTGACTGCCAGCTTGCGCCACAGCTCGGCGCGAATATTGTTATGCCAGGCAACGTCAGGCAACACGGTTTGCAAAATATCCGCCAGATAACTGTAATCCCCGTCCTGTTGCCGTGCCGGGCCAATATGCGTGATACCGTTTGCCACATGAATAATGACATTGCCGTCGCGGCGGGCTGCATGGGTGGTGGTGCCCATCAGTAATGGCTGCTGAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGT  >  NC_000913/443955‑444223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑