Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,655,360 (C)5→4 coding (314/327 nt) ynfA ← UPF0060 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,655,3560C.100.0% 148.7 / NA 36coding (318/327 nt)ynfAUPF0060 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (17/19);  total (17/19)

AAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCA  >  NC_000913/1655220‑1655485
                                                                                                                                        |                                                                                                                                 
aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccc                                                                                                                                 >  1:539313/1‑139 (MQ=255)
                             gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCa                                                                                                   >  1:117524/1‑139 (MQ=255)
                                tatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                            >  1:1076453/1‑108 (MQ=255)
                                tatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                            <  2:1076453/112‑5 (MQ=255)
                                    tGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCa                                                                                            <  2:539313/139‑1 (MQ=255)
                                          gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGcccca                                                                                                                               <  1:1333467/99‑2 (MQ=255)
                                          gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGcccca                                                                                                                               >  2:1333467/1‑98 (MQ=255)
                                              aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAACTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                  <  1:651592/139‑1 (MQ=255)
                                                  ttGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                             >  1:1060886/1‑124 (MQ=255)
                                                  ttGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                             <  2:1060886/124‑1 (MQ=255)
                                                   tGACAAAAGATGAAATAGAATACCTCTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATCTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                             <  2:618299/139‑1 (MQ=255)
                                                     aCAAAAGATGAAATAGAATGCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCa                                                                                            >  1:1022418/1‑122 (MQ=255)
                                                     aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCa                                                                                            <  2:1022418/122‑1 (MQ=255)
                                                     aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                           <  2:54089/139‑1 (MQ=255)
                                                     aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                           >  1:54089/1‑139 (MQ=255)
                                                            aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                    <  2:117524/139‑1 (MQ=255)
                                                                 aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                               <  2:1241204/139‑1 (MQ=255)
                                                                             ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                     <  1:1422236/137‑1 (MQ=255)
                                                                             ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                     >  2:1422236/1‑137 (MQ=255)
                                                                               gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGAt                                                                                                               >  2:900648/1‑77 (MQ=255)
                                                                               gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGAt                                                                                                               <  1:900648/77‑1 (MQ=255)
                                                                                    cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAGAGAGTCAGTTTCACGCCATCcaca                                            >  1:857310/1‑139 (MQ=255)
                                                                                        cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGcccca                                                                                                                               <  1:702107/53‑2 (MQ=255)
                                                                                        cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGcccca                                                                                                                               >  2:702107/1‑52 (MQ=255)
                                                                                                   atcttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGc                                                                                                    >  1:1206031/1‑68 (MQ=255)
                                                                                                   atcttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGc                                                                                                    <  2:1206031/68‑1 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                      <  2:1371747/45‑1 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                      >  1:1371747/1‑45 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                             >  1:1000007/1‑86 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                             <  2:274420/86‑1 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                             >  1:274420/1‑86 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATcc                                               <  1:269776/116‑1 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATcc                                               >  2:269776/1‑116 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTATTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                             <  2:1000007/86‑1 (MQ=255)
                                                                                                           gATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                          >  2:1312004/1‑102 (MQ=255)
                                                                                                           gATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                          <  1:1312004/102‑1 (MQ=255)
                                                                                                              aaaaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATg                                                                                                        <  2:528710/53‑1 (MQ=255)
                                                                                                              aaaaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATg                                                                                                        >  1:528710/1‑53 (MQ=255)
                                                                                                                         aTGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGc       <  1:1384227/139‑1 (MQ=255)
                                                                                                                              cttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                             >  1:1049624/3‑64 (MQ=39)
                                                                                                                              cttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                             <  2:1049624/62‑1 (MQ=39)
                                                                                                                              cttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGGCATAAACGccacca  >  1:1216107/3‑139 (MQ=255)
                                                                                                                              cttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca  >  2:711964/3‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                 
AAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCA  >  NC_000913/1655220‑1655485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: