Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,022	(T)6→5	coding (68/1284 nt)	yeaH →	UPF0229 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,017	0	T	.	100.0%	136.3 / NA	33	coding (63/1284 nt)	yeaH	UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (15/18);  total (15/18)

ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGA  >  NC_000913/1868882‑1869141                                                                                                                                        |                                                                                                                             aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttt                                                                                                                           >  2:725149/1‑139 (MQ=255) aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttt                                                                                                                           >  2:357802/1‑139 (MQ=255)   aGCGAACCGTGGGCCTGAGAAGCGACAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         <  1:855865/139‑2 (MQ=255)     cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       <  2:1480718/139‑4 (MQ=255)     cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       >  1:956605/1‑136 (MQ=255)     cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       <  1:725149/139‑4 (MQ=255)            tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       >  1:1456186/1‑129 (MQ=255)            tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       <  2:1456186/132‑4 (MQ=255)                 cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                          <  2:659550/139‑1 (MQ=255)                         cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       <  2:173028/119‑4 (MQ=255)                         cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       >  1:173028/1‑116 (MQ=255)                           gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                <  1:357802/139‑1 (MQ=255)                                       tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                    <  2:956605/139‑1 (MQ=255)                                               aGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAt                                                                            >  1:537735/1‑139 (MQ=255)                                                gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                >  2:1218300/1‑134 (MQ=255)                                                gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                <  1:1218300/134‑1 (MQ=255)                                                  tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         >  2:346811/1‑91 (MQ=255)                                                  tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         <  1:346811/92‑2 (MQ=255)                                                   ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgcg                                                                                                         <  1:1234835/106‑1 (MQ=255)                                                   ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgcg                                                                                                         >  2:1234835/1‑106 (MQ=255)                                                                   ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       >  2:1232618/1‑74 (MQ=255)                                                                   ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                       <  1:1232618/77‑4 (MQ=255)                                                                   ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         >  2:340948/1‑74 (MQ=255)                                                                   ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         <  1:340948/75‑2 (MQ=255)                                                                   ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                          <  1:5753/105‑1 (MQ=255)                                                                   ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                          >  2:5753/1‑105 (MQ=255)                                                                   ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                        >  2:404976/1‑139 (MQ=255)                                                                          atGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGa                                                 <  2:197564/139‑1 (MQ=255)                                                                               cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         <  1:179392/63‑2 (MQ=255)                                                                               cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         >  2:179392/1‑62 (MQ=255)                                                                                  gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                          >  1:798528/1‑74 (MQ=255)                                                                                  gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                          <  2:798528/74‑1 (MQ=255)                                                                                   tttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         <  2:1136515/59‑2 (MQ=255)                                                                                   tttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                         >  1:1136515/1‑58 (MQ=255)                                                                                    ttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgcgc                                                                                                        <  2:956379/74‑1 (MQ=255)                                                                                    ttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgcgc                                                                                                        >  1:956379/1‑74 (MQ=255)                                                                                       ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                           >  1:37252/1‑116 (MQ=255)                                                                                       ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                           <  2:37252/116‑1 (MQ=255)                                                                                       ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                    <  1:1288840/139‑1 (MQ=255)                                                                                            cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg                                                                     <  2:482532/101‑1 (MQ=255)                                                                                            cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg                                                                     >  1:482532/1‑101 (MQ=255)                                                                                            cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                    >  1:1246731/1‑134 (MQ=255)                                                                                            cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                    <  2:1246731/134‑1 (MQ=255)                                                                                                 tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                         >  2:666432/1‑124 (MQ=255)                                                                                                 tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                         <  1:666432/124‑1 (MQ=255)                                                                                                    gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                       <  2:537735/139‑1 (MQ=255)                                                                                                    gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                       <  1:1415928/139‑1 (MQ=255)                                                                                                    gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                       <  2:837816/139‑1 (MQ=255)                                                                                                               aCAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATAtt            >  2:1519592/1‑139 (MQ=255)                                                                                                                    aGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGa       >  2:1449143/1‑139 (MQ=255)                                                                                                                         ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGt                                                                                            <  2:179027/49‑1 (MQ=255)                                                                                                                         ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGt                                                                                            >  1:179027/1‑49 (MQ=255)                                                                                                                         ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa  >  1:482469/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                             ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGA  >  NC_000913/1868882‑1869141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑