Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 321,564 | (G)7→6 | intergenic (+483/‑44) | rclR → / → ykgE | reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 321,558 | 0 | G | . | 97.9% | 190.6 / ‑2.3 | 47 | intergenic (+477/‑50) | rclR/ykgE | reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component |
Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (24/22); total (25/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 |
TGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC > NC_000913/321454‑321692 | tGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACa < 2:519383/115‑1 (MQ=255) tGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACa > 1:519383/1‑115 (MQ=255) gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg < 2:1530008/139‑1 (MQ=255) ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCgg > 1:1370337/1‑113 (MQ=255) ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCgg < 2:1370337/113‑1 (MQ=255) aTCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAAt > 1:802204/1‑139 (MQ=255) tCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATg < 2:623648/114‑1 (MQ=255) tCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATg > 1:623648/1‑114 (MQ=255) aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACa > 2:733041/1‑83 (MQ=255) aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACa < 1:733041/83‑1 (MQ=255) aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc < 2:1534621/139‑1 (MQ=255) gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc > 1:799560/1‑133 (MQ=255) gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc < 2:799560/133‑1 (MQ=255) tGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc > 1:413451/1‑125 (MQ=255) tGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc < 2:413451/125‑1 (MQ=255) gTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGAc > 1:943301/1‑139 (MQ=255) aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg > 1:12907/1‑139 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACAt < 1:678810/59‑1 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt > 1:733527/1‑84 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt < 2:733527/84‑1 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTg < 2:1073979/130‑1 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTg > 1:1073979/1‑130 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATGGATGATTGATCCCCCGGT‑GGGGGGAAACAt > 2:678810/1‑59 (MQ=255) ccAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGaa < 2:1355531/139‑1 (MQ=255) ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa > 2:1176057/1‑139 (MQ=255) aaCGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCa > 2:26851/1‑139 (MQ=255) attGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtg < 2:12907/139‑1 (MQ=255) attGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtg > 2:277431/1‑139 (MQ=255) gatgatTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg > 2:80110/1‑126 (MQ=255) gatgatTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg < 1:80110/126‑1 (MQ=255) tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTgctgct < 2:802204/139‑1 (MQ=255) tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTgctgct < 1:26851/139‑1 (MQ=255) gattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa < 1:415269/58‑1 (MQ=255) gattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa > 2:415269/1‑58 (MQ=255) attgatCCCCCGGTGGGGGGG‑AACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg > 1:1042073/1‑105 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa > 2:874989/1‑50 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa < 1:874989/50‑1 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc > 1:1320530/1‑84 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc < 2:1320530/84‑1 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg < 2:1042073/105‑1 (MQ=255) attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc > 2:395524/1‑139 (MQ=255) atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCt > 2:441755/1‑100 (MQ=255) atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCt < 1:441755/100‑1 (MQ=255) atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGa > 2:410043/1‑139 (MQ=255) ccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc > 1:1462395/1‑77 (MQ=255) ccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc < 2:1462395/77‑1 (MQ=255) ccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAc > 2:598402/1‑139 (MQ=255) | TGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC > NC_000913/321454‑321692 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |