Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,085,994 (GT)5→4 intergenic (‑172/‑21) fdoG ← / → fdhD formate dehydrogenase‑O, large subunit/formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,085,9860G.100.0% 114.7 / NA 28intergenic (‑164/‑30)fdoG/fdhDformate dehydrogenase‑O, large subunit/formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/14);  total (14/14)
*NC_0009134,085,9870T.100.0% 114.7 / NA 28intergenic (‑165/‑29)fdoG/fdhDformate dehydrogenase‑O, large subunit/formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/14);  total (14/14)

ACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGGTGTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTAC  >  NC_000913/4085849‑4086103
                                                                                                                                         ||                                                                                                                    
aCGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgt                                                                                                                      <  1:1335485/139‑1 (MQ=255)
  gTCCCATTTACGTTCGCTTGCTGCGTGTGCAGGTAGTGTGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgt                                                                                                                    <  2:588599/139‑1 (MQ=255)
  gTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgt                                                                                                                    <  2:1045908/139‑1 (MQ=255)
        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgtgtgttc                                                                                                              >  2:171290/1‑137 (MQ=255)
        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgt                                                                                                                    <  2:869722/133‑1 (MQ=255)
        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgt                                                                                                                    >  1:869722/1‑133 (MQ=255)
               tCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGTCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgt                                                                                                                    <  2:526030/126‑1 (MQ=255)
               tCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGTCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgt                                                                                                                    >  1:526030/1‑126 (MQ=255)
                         cGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgtgtgttcttat                                                                                                          <  1:245749/126‑7 (MQ=255)
                         cGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgtgtgttcttat                                                                                                          >  2:245749/1‑120 (MQ=255)
                                     gtgggagtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAac                                                                               <  1:171290/139‑1 (MQ=255)
                                      tgggagtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGt                                                                                        >  1:1503947/1‑129 (MQ=255)
                                      tgggagtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGt                                                                                        <  2:1503947/129‑1 (MQ=255)
                                        ggagtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAAcaca                                                                            <  2:1224909/139‑1 (MQ=255)
                                                 tttCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGa                                                                   >  2:79941/1‑139 (MQ=255)
                                                                 gCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACa                                                   <  1:79941/139‑1 (MQ=255)
                                                                   cGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgtgtgttcttat                                                                                                          >  1:679185/1‑78 (MQ=255)
                                                                   cGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgtgtgttcttat                                                                                                          <  2:679185/84‑7 (MQ=255)
                                                                          gTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgtgtgttcttat                                                                                                          <  1:783730/77‑7 (MQ=255)
                                                                          gTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgtgtgttcttat                                                                                                          >  2:783730/1‑71 (MQ=255)
                                                                                    ggCTTAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgtgtgttcttat                                                                                                          <  1:911228/67‑7 (MQ=255)
                                                                                    ggCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGgtgtgtgttcttat                                                                                                          >  2:911228/1‑61 (MQ=255)
                                                                                    ggCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACa                                                   <  2:136179/120‑1 (MQ=255)
                                                                                    ggCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACa                                                   >  1:136179/1‑120 (MQ=255)
                                                                                    ggCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAata                                               <  2:260191/124‑1 (MQ=255)
                                                                                    ggCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAata                                               >  1:260191/1‑124 (MQ=255)
                                                                                    ggCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTc                                >  2:324060/1‑139 (MQ=255)
                                                                                     gCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAAtgtg                                                      >  2:1241618/1‑116 (MQ=255)
                                                                                     gCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAAtgtg                                                      <  1:1241618/116‑1 (MQ=255)
                                                                                                   catccaGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGt                                 >  2:522725/1‑123 (MQ=255)
                                                                                                   catccaGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGt                                 <  1:522725/123‑1 (MQ=255)
                                                                                                     tccaGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTa                     <  1:692351/133‑1 (MQ=255)
                                                                                                     tccaGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTa                     >  2:692351/1‑133 (MQ=255)
                                                                                                     tccaGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATg                   <  2:1397039/135‑1 (MQ=255)
                                                                                                     tccaGGAAGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATg                   >  1:1397039/1‑135 (MQ=255)
                                                                                                            aGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATca                                            <  2:176661/103‑1 (MQ=255)
                                                                                                            aGGGTTTGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATca                                            >  1:176661/1‑103 (MQ=255)
                                                                                                                  tGTAACAGAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTac  >  2:210104/1‑139 (MQ=255)
                                                                                                                         gAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATcac                                           >  2:1317627/1‑91 (MQ=255)
                                                                                                                         gAATACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATcac                                           <  1:1317627/91‑1 (MQ=255)
                                                                                                                          aaTACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTc                                <  2:536465/101‑1 (MQ=255)
                                                                                                                          aaTACCATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTc                                >  1:536465/1‑101 (MQ=255)
                                                                                                                               cATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAg                        <  2:466669/104‑1 (MQ=255)
                                                                                                                               cATAATGTTG‑‑GTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAg                        >  1:466669/1‑104 (MQ=255)
                                                                                                                                         ||                                                                                                                    
ACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTTGGTGTGTGTGTTCTTATCTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTAC  >  NC_000913/4085849‑4086103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: