| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 7,925 | +A | coding (35/1431 nt) | yaaJ ← | putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 7,922 | 1 | . | A | 97.9% | 150.0 / ‑3.6 | 48 | coding (38/1431 nt) | yaaJ | putative transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (23/24); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑AA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCT > NC_000913/7791‑8055 | tgCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aa‑aagga < 1:437313/138‑5 (MQ=255)cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aa‑aagga < 1:160410/139‑5 (MQ=255)cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aa‑aagga < 1:526532/139‑5 (MQ=255) ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACg < 2:414701/139‑1 (MQ=255) ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACg < 1:749346/139‑1 (MQ=255) tgGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAAt > 1:1104611/1‑139 (MQ=255) tttttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGaaaaaa > 2:146028/1‑139 (MQ=255) tttttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aa‑aagga < 2:346858/116‑5 (MQ=255) tttttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aa‑aagga > 1:346858/1‑112 (MQ=255) ttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCt < 2:1104611/139‑1 (MQ=255) ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat > 2:1022269/1‑139 (MQ=255) ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat > 2:894992/1‑139 (MQ=255) ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat > 2:583391/1‑139 (MQ=255) cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACg < 2:249999/85‑1 (MQ=255) cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACg > 1:249999/1‑85 (MQ=255) cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgc < 2:1355593/139‑1 (MQ=255) aaaCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa < 1:583391/139‑1 (MQ=255) aCTGCACAAATCCGGTGCGAAAAGTGACCCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATg < 2:884463/139‑1 (MQ=255) cTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACg < 2:874377/80‑1 (MQ=255) cTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACg > 1:874377/1‑80 (MQ=255) caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgc < 1:1048535/102‑1 (MQ=255) caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgc > 2:1048535/1‑102 (MQ=255) caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc < 1:797691/139‑1 (MQ=255) caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc < 1:146028/139‑1 (MQ=255) aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATccc < 1:1178342/110‑1 (MQ=255) aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATccc > 2:1178342/1‑110 (MQ=255) ggTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTa < 1:894992/139‑1 (MQ=255) gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACg < 2:505344/67‑1 (MQ=255) gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACg > 1:505344/1‑67 (MQ=255) aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATGATTACGGATCCCCAAA‑AGGACg > 2:48467/1‑62 (MQ=255) aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACg < 1:48467/62‑1 (MQ=255) aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgc > 1:1477382/1‑116 (MQ=255) aaaaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgc < 2:1477382/116‑1 (MQ=255) cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgc < 1:25886/69‑1 (MQ=255) cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgc > 2:25886/1‑69 (MQ=255) aaGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa < 1:117204/127‑1 (MQ=255) aaGAGCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa > 2:117204/1‑127 (MQ=255) agagCAGGTAAATCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGc > 2:804987/1‑139 (MQ=255) aaTCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca < 2:377168/58‑1 (MQ=255) aaTCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca > 1:377168/1‑58 (MQ=255) tCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa > 2:1352362/1‑80 (MQ=255) tCATTACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGa < 1:1352362/80‑1 (MQ=255) ttACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc > 1:265097/1‑85 (MQ=255) ttACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc < 2:265097/85‑1 (MQ=255) tACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCct > 2:549028/1‑59 (MQ=255) tACCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCct < 1:549028/59‑1 (MQ=255) aCCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg < 2:207855/126‑1 (MQ=255) aCCGATCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg > 1:207855/1‑126 (MQ=255) ccGATCCCCAAA‑AGGACGCTGTTAATGAAGGAAAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCTCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAAGTACTTGATAAc > 2:1439423/1‑139 (MQ=255) gATCCCC‑AACAGGACGCTGTTAATGAAGGAGAGAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACtt < 2:1187697/139‑1 (MQ=255) atcccca‑AA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat < 2:199444/45‑1 (MQ=255) atcccca‑AA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa > 2:528627/4‑105 (MQ=255) tcccca‑AA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc > 1:1164198/3‑78 (MQ=255) tcccca‑AA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCt < 1:1022269/137‑1 (MQ=255) tcccca‑AA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGACATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc < 2:1164198/76‑1 (MQ=255) aTCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgcat > 1:199444/1‑48 (MQ=255) aTCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa < 1:528627/105‑1 (MQ=255) tCCCCAAA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCt > 1:40133/1‑139 (MQ=255) ccca‑AA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCCTATTGCCGGTCGCGAGGACTTTCCTGTGTAAACGTTACCAATTGt < 2:887159/98‑1 (MQ=255) | CGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑AA‑AGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCT > NC_000913/7791‑8055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |