Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,380,329	(G)5→6	coding (182/660 nt)	ycjU →	beta‑phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,380,324	1	.	G	100.0%	185.3 / NA	53	H59Q (CAC→CAG)	ycjU	beta‑phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/27);  total (26/27)

ATCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑GGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGCTCTTTGC  >  NC_000913/1380187‑1380451                                                                                                                                           |                                                                                                                                aTCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑g                                                                                                                                <  1:1083539/139‑1 (MQ=255)  tCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑gg                                                                                                                               >  1:749510/1‑139 (MQ=255)  tCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑gg                                                                                                                               >  1:1480070/1‑139 (MQ=255)            cgcgCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAacac                                                                                                                                  >  2:836411/1‑127 (MQ=255)            cgcgCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAacac                                                                                                                                  <  1:836411/127‑1 (MQ=255)              cgcATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAgg                                                                                                                    >  2:856733/1‑139 (MQ=255)              cgcATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAgg                                                                                                                    >  1:876967/1‑139 (MQ=255)               gcATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAggg                                                                                                                   <  1:823877/139‑1 (MQ=255)                cATCTGCATTTCCAGGCGTGGCAGCGGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGc                                                                                                                  <  1:1440600/139‑1 (MQ=255)                cATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGc                                                                                                                  >  1:548422/1‑139 (MQ=255)                       aTTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTa                                                                                                           <  1:856733/139‑1 (MQ=255)                       aTTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTa                                                                                                           <  2:1480070/139‑1 (MQ=255)                           ccAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTc                                                                                                       <  2:876967/139‑1 (MQ=255)                              ggCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCa                                                                                                    >  1:1266191/1‑139 (MQ=255)                                cGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAgg                                                                                                  >  2:942216/1‑139 (MQ=255)                                  tGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGgag                                                                                                <  1:659014/139‑1 (MQ=255)                                      agcagATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAggg                                                                                            <  2:548422/139‑1 (MQ=255)                                               ccGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTgg                                                                                   >  2:1455555/1‑139 (MQ=255)                                                              tCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATc                                                                    <  2:147309/139‑1 (MQ=255)                                                                 gCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGc                                                                 >  1:905196/1‑139 (MQ=255)                                                                     tGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTa                                                             >  1:641383/1‑139 (MQ=255)                                                                       aTGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATg                                                           <  2:749510/139‑1 (MQ=255)                                                                               tttAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAggg                                                                                                                   >  2:814474/1‑75 (MQ=255)                                                                               tttAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAggg                                                                                                                   <  1:814474/75‑1 (MQ=255)                                                                                   aCGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTAc                                               <  2:1237343/139‑1 (MQ=255)                                                                                        tCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                     >  1:1360521/1‑112 (MQ=255)                                                                                        tCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                     <  2:1360521/112‑1 (MQ=255)                                                                                        tCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAg                                          <  2:1266191/139‑1 (MQ=255)                                                                                         cccTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAacac                                                                                                                                  >  1:1251891/1‑50 (MQ=255)                                                                                         cccTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAacac                                                                                                                                  <  2:1251891/50‑1 (MQ=255)                                                                                         cccTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGga                                                                                                 >  1:1348160/1‑83 (MQ=255)                                                                                         cccTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGga                                                                                                 <  2:1348160/83‑1 (MQ=255)                                                                                           cTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAGGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTg                                       <  2:905196/139‑1 (MQ=255)                                                                                                  ggATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTATAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCa                                >  1:799510/1‑139 (MQ=255)                                                                                                   gATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTa                                                             <  1:315276/109‑1 (MQ=255)                                                                                                   gATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTa                                                             >  2:315276/1‑109 (MQ=255)                                                                                                   gATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCaa                               >  1:69553/1‑139 (MQ=255)                                                                                                    aTCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                     >  1:1083173/1‑100 (MQ=255)                                                                                                    aTCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                     <  2:1083173/100‑1 (MQ=255)                                                                                                     tCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCTCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACg                             <  1:194002/139‑1 (MQ=255)                                                                                                     tCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACg                             >  1:25109/1‑139 (MQ=255)                                                                                                       aGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCt                           >  2:616695/1‑139 (MQ=255)                                                                                                         ccGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTc                                                                                                       <  1:1113136/61‑1 (MQ=255)                                                                                                         ccGCGATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTc                                                                                                       >  2:1113136/1‑61 (MQ=255)                                                                                                            cgATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGctc                                                               <  1:1233396/98‑1 (MQ=255)                                                                                                            cgATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGctc                                                               >  2:1233396/1‑98 (MQ=255)                                                                                                             gATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAggg                                                                                                                   <  2:187649/45‑1 (MQ=255)                                                                                                             gATGAGTCTCTGCGGCGCATTCTGCAACACGGGGGGCAAAGAggg                                                                                                                   >  1:187649/1‑45 (MQ=255)                                                                                                                   tctcTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGc                                                                                                                  >  2:727859/1‑40 (MQ=255)                                                                                                                   tctcTGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGc                                                                                                                  <  1:727859/40‑1 (MQ=255)                                                                                                                      ctGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCcactc                                                    >  2:412827/1‑99 (MQ=255)                                                                                                                      ctGCGGCGCATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCcactc                                                    <  1:412827/99‑1 (MQ=255)                                                                                                                           gcgcATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGctc                                                               >  2:1238088/1‑83 (MQ=255)                                                                                                                           gcgcATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGctc                                                               <  1:1238088/83‑1 (MQ=255)                                                                                                                           gcgcATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGctc       >  1:287379/1‑139 (MQ=255)                                                                                                                            cgcATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                     <  2:1362418/76‑1 (MQ=255)                                                                                                                            cgcATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAAt                                                                     >  1:1362418/1‑76 (MQ=255)                                                                                                                              cATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGc                                                                 <  2:1132533/78‑1 (MQ=255)                                                                                                                              cATTCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGc                                                                 >  1:1132533/1‑78 (MQ=255)                                                                                                                                ttCTGCAACACGGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGCTCTTTGc  <  1:1455555/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                ATCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGCGGCGCATTCTGCAACAC‑GGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTGCTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGCTCTTTGC  >  NC_000913/1380187‑1380451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑