Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,399,985 (C)7→8 coding (252/516 nt) ogt ← O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,399,9781.C100.0% 129.9 / NA 39T87A (ACG→GCG) ogtO‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/22);  total (17/22)

TCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC  >  NC_000913/1399846‑1400111
                                                                                                                                     |                                                                                                                                     
tCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcccccc                                                                                                                                 <  2:756720/139‑1 (MQ=255)
   aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTccccccccg                                                                                                                              <  2:1516228/139‑3 (MQ=255)
              gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                                    <  2:1123581/139‑1 (MQ=255)
              gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                                    >  1:28999/1‑139 (MQ=255)
                aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg                                                                                                                  <  2:116931/139‑1 (MQ=255)
                aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg                                                                                                                  <  2:1146535/139‑1 (MQ=255)
                  cAACGGCACGCGCCGCGCCAGGCCGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcc                                                                                                                                     >  2:1193156/1‑117 (MQ=255)
                  cAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcc                                                                                                                                     <  1:1193156/117‑1 (MQ=255)
                          cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaata                                                                                                        >  1:415046/1‑139 (MQ=255)
                           gcgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaataa                                                                                                       <  2:415046/139‑1 (MQ=255)
                                gcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTa                                                                                                  >  1:510073/1‑139 (MQ=255)
                                            cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa                                                                                      >  2:960626/1‑139 (MQ=255)
                                            cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTccccccccgta                                                                                                                            <  2:77203/100‑5 (MQ=255)
                                            cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTccccccccgta                                                                                                                            >  1:77203/1‑96 (MQ=255)
                                                             ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCGAAATATTCACGAAGCtt                                                                     <  1:960626/139‑1 (MQ=255)
                                                              tGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTg                                                                    <  1:1336287/139‑1 (MQ=255)
                                                                       tGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg                                                                                                                  >  2:135095/1‑84 (MQ=255)
                                                                       tGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg                                                                                                                  <  1:135095/84‑1 (MQ=255)
                                                                          aTTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaata                                                                                                        <  2:1150544/91‑1 (MQ=255)
                                                                          aTTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaata                                                                                                        >  1:1150544/1‑91 (MQ=255)
                                                                            tACCTGCCCGCTGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGcc                                                      <  1:994906/139‑1 (MQ=255)
                                                                              ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGc                                                                                                                           >  1:1024611/1‑68 (MQ=255)
                                                                              ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGc                                                                                                                           <  2:1024611/68‑1 (MQ=255)
                                                                                  cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                                    >  2:416928/1‑71 (MQ=255)
                                                                                  cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                                    <  1:416928/71‑1 (MQ=255)
                                                                                   ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGc                                                                                                                           >  2:612425/1‑63 (MQ=255)
                                                                                   ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGc                                                                                                                           <  1:612425/63‑1 (MQ=255)
                                                                                   ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaa                                                                                                          >  2:39593/1‑80 (MQ=255)
                                                                                   ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaa                                                                                                          <  1:39593/80‑1 (MQ=255)
                                                                                   ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcccc                                                                                                                                   >  2:276702/1‑54 (MQ=255)
                                                                                   ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcccc                                                                                                                                   <  1:276702/54‑1 (MQ=255)
                                                                                     gCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtggt                                             <  2:1259448/139‑1 (MQ=255)
                                                                                                 gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCAc                                                                            <  1:657168/96‑1 (MQ=255)
                                                                                                 gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCAc                                                                            >  2:657168/1‑96 (MQ=255)
                                                                                                  cGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                                    >  1:325829/1‑55 (MQ=255)
                                                                                                  cGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                                    <  2:325829/55‑1 (MQ=255)
                                                                                                  acaggttttttCGAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATACACGAGGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc                                <  1:915110/132‑1 (MQ=255)
                                                                                                    tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTc                              >  1:828193/1‑139 (MQ=255)
                                                                                                    tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcccc                                                                                                                                   <  2:1097161/37‑1 (MQ=255)
                                                                                                    tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcccc                                                                                                                                   >  1:1097161/1‑37 (MQ=255)
                                                                                                     aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCa                             >  2:204224/1‑139 (MQ=255)
                                                                                                     aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcccc                                                                                                                                   >  2:1256726/1‑36 (MQ=255)
                                                                                                     aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcccc                                                                                                                                   <  1:1256726/36‑1 (MQ=255)
                                                                                                         ttttCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg                                                                                                                  <  1:198657/50‑1 (MQ=255)
                                                                                                         ttttCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg                                                                                                                  >  2:198657/1‑50 (MQ=255)
                                                                                                         ttttCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGc                         <  2:828193/139‑1 (MQ=255)
                                                                                                          tttCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATAtt                                                                               >  1:1485950/1‑84 (MQ=255)
                                                                                                          tttCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATAtt                                                                               <  2:1485950/84‑1 (MQ=255)
                                                                                                                 aCTTCGCGCTGAAATGGCGTCCCCCCCCATACCTGTGGGAAGCTTAGCAATAATGCTAATATTACCGGCAAAATATTCACGATGCTTGTCGCTTAAGCCACCTGGATTGGTGGCAGAAATGCGCTCATAGCctacctgt                 >  2:915110/1‑133 (MQ=255)
                                                                                                                                ggCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc  <  2:28999/139‑1 (MQ=255)
                                                                                                                                ggCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc  <  2:510073/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC  >  NC_000913/1399846‑1400111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: