Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,285,401	+G	coding (1026/1761 nt)	yejM →	essential inner membrane DUF3413 domain‑containing protein, lipid A production and membrane permeability factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,285,397	1	.	G	100.0%	169.5 / NA	49	L341R (CTG→CGG)	yejM	essential inner membrane DUF3413 domain‑containing protein, lipid A production and membrane permeability factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (25/24);  total (25/24)

ATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑GGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGG  >  NC_000913/2285259‑2285526                                                                                                                                            |                                                                                                                                  atatGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCt                                                                                                                                    <  2:221318/139‑1 (MQ=255) atatGAGCTCCGGCAACACTACAGACAACAGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCTGCt                                                                                                                                    <  2:989780/139‑1 (MQ=255)          ccGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑gggggttat                                                                                                                          >  2:24398/1‑134 (MQ=255)                   cTACAGACAATGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  2:748395/139‑1 (MQ=255)                   cTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  1:273638/139‑1 (MQ=255)                   cTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  2:1256129/139‑1 (MQ=255)                     aCAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGAt                                                                                                                >  1:124159/1‑139 (MQ=255)                           aaCGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCttt                                                                                                          >  2:1320865/1‑139 (MQ=255)                             cGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGAGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTAc                                                                                                        >  2:878890/1‑139 (MQ=255)                                cATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATACTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑ggggg                                                                                                                              <  1:1223376/113‑2 (MQ=255)                                cATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATACTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑ggggg                                                                                                                              >  2:1223376/1‑112 (MQ=255)                                      tGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCt                                                                                               <  1:878890/139‑1 (MQ=255)                                        gCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGt                                                                                             <  2:1304279/139‑1 (MQ=255)                                           tGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATc                                                                                          <  1:1320865/139‑1 (MQ=255)                                             ttCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGc                                                                                        >  1:1509682/1‑139 (MQ=255)                                                       tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAttgt                                                                              >  2:1329466/1‑139 (MQ=255)                                                       tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAttgt                                                                              >  2:1500021/1‑139 (MQ=255)                                                       tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAttgt                                                                              >  2:1094491/1‑139 (MQ=255)                                                       tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTAAGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGATGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAttgt                                                                              >  1:1335128/1‑139 (MQ=255)                                                              gAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGa                                                                       <  1:24398/139‑1 (MQ=255)                                                                   atatGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCg                                                                                         <  2:182938/116‑1 (MQ=255)                                                                   atatGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCg                                                                                         >  1:182938/1‑116 (MQ=255)                                                                        gACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATg                                                             <  2:162016/139‑1 (MQ=255)                                                                           ggCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  1:51468/83‑1 (MQ=255)                                                                           ggCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  >  2:51468/1‑83 (MQ=255)                                                                           ggCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCg                                                          >  1:1433988/1‑139 (MQ=255)                                                                           ggCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCg                                                          >  1:1199983/1‑139 (MQ=255)                                                                              aTTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCTGCTGTATCGCCAGGCa                                                                                  >  2:1218616/1‑112 (MQ=255)                                                                              aTTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCTGCTGTATCGCCAGGCa                                                                                  <  1:1218616/112‑1 (MQ=255)                                                                                    tCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGc                                                 <  1:1094491/139‑1 (MQ=255)                                                                                            tACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATgg                                                                                                              <  2:821426/70‑1 (MQ=255)                                                                                            tACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATgg                                                                                                              >  1:821426/1‑70 (MQ=255)                                                                                                  tGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  1:384518/60‑1 (MQ=255)                                                                                                  tGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  >  2:384518/1‑60 (MQ=255)                                                                                                         ttaattaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATc                                                                                          >  2:615474/1‑77 (MQ=255)                                                                                                         ttaattaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATc                                                                                          <  1:615474/77‑1 (MQ=255)                                                                                                          taattaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCg                           >  1:368551/1‑139 (MQ=255)                                                                                                            attaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGcc                         <  1:1329466/139‑1 (MQ=255)                                                                                                                cTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGc                     <  1:1500021/139‑1 (MQ=255)                                                                                                                cTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGc                     <  2:368551/139‑1 (MQ=255)                                                                                                                 tGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCa                                                                                  <  2:588517/77‑1 (MQ=255)                                                                                                                 tGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCa                                                                                  >  1:588517/1‑77 (MQ=255)                                                                                                                 tGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAg                                  <  1:1203534/125‑1 (MQ=255)                                                                                                                 tGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAg                                  >  2:1203534/1‑125 (MQ=255)                                                                                                                   cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTAc                                                   >  1:463621/1‑106 (MQ=255)                                                                                                                   cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTAc                                                   <  2:463621/106‑1 (MQ=255)                                                                                                                   cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGt                  >  2:140806/1‑139 (MQ=255)                                                                                                                     cTTAATCAGCGAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTgg                >  1:50166/1‑139 (MQ=255)                                                                                                                     cTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCACTgg                >  1:818227/1‑139 (MQ=255)                                                                                                                              cAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGa                                                                       <  2:83659/75‑1 (MQ=255)                                                                                                                              cAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGa                                                                       >  1:83659/1‑75 (MQ=255)                                                                                                                                  gCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTa                                                                                            <  1:1308186/50‑1 (MQ=255)                                                                                                                                  gCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTa                                                                                            >  2:1308186/1‑50 (MQ=255)                                                                                                                                   cTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTggg  <  2:1199983/139‑1 (MQ=255)                                                                                                                                            |                                                                                                                                  ATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑GGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGG  >  NC_000913/2285259‑2285526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑