Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,568,105	+C	coding (3/1227 nt)	eutH ←	ethanolamine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,568,102	1	.	C	100.0%	176.2 / NA	50	G2G (GGA→GGG)	eutH	ethanolamine transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (27/23);  total (27/23)

ACCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑CCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGC  >  NC_000913/2567970‑2568232                                                                                                                                      |                                                                                                                                   aCCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑ccccat                                                                                                                              >  2:1162591/1‑136 (MQ=255) aCCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑ccccat                                                                                                                              >  2:615942/1‑136 (MQ=255) aCCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑ccccat                                                                                                                              >  1:1502849/1‑136 (MQ=255) aCCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑ccccat                                                                                                                              <  1:615942/139‑4 (MQ=255)         aTCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTa                                                                                                                       <  2:658338/139‑1 (MQ=255)           ccTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACt                                                                                                                     <  1:1162591/139‑1 (MQ=255)           ccTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACt                                                                                                                     <  2:74039/139‑1 (MQ=255)                     ttttACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAgt                                                                                                           >  1:1472623/1‑139 (MQ=255)                                     gAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑cccc                                                                                                                                <  2:1508315/101‑2 (MQ=255)                                     gAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑cccc                                                                                                                                >  1:1508315/1‑100 (MQ=255)                                                   aaCCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACg                                                                             >  1:1522237/1‑139 (MQ=255)                                                          gAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATc                                                                      <  2:1502849/139‑1 (MQ=255)                                                                      gATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTa                                                                        <  2:140654/125‑1 (MQ=255)                                                                      gATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTa                                                                        >  1:140654/1‑125 (MQ=255)                                                                      gATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACAtgt                                                          >  2:1438104/1‑139 (MQ=255)                                                                             tCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATcc                                                   >  2:1270984/1‑139 (MQ=255)                                                                                 cGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGc                                                                                             >  2:581887/1‑93 (MQ=255)                                                                                 cGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGc                                                                                             <  1:581887/93‑1 (MQ=255)                                                                                 cGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGTGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa                                               >  1:1505976/1‑139 (MQ=255)                                                                                   gCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg                                             <  2:1472623/139‑1 (MQ=255)                                                                                    cAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTc                                                                                                                    >  1:52939/1‑67 (MQ=255)                                                                                    cAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTc                                                                                                                    <  2:52939/67‑1 (MQ=255)                                                                                    cAGATATCAGCATAAAGAACGTCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACAGGTGCAATCCCGTAGGc                                            >  1:1042521/1‑139 (MQ=38)                                                                                       cTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGt                                                                                                               >  1:603247/1‑69 (MQ=255)                                                                                       cTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGt                                                                                                               <  2:603247/69‑1 (MQ=255)                                                                                       cTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCt                                                                <  1:1214089/116‑1 (MQ=255)                                                                                       cTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCt                                                                >  2:1214089/1‑116 (MQ=255)                                                                                       cTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTgg                                         >  2:1364628/1‑139 (MQ=255)                                                                                         aTCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATg                                                                                        >  2:1173241/1‑90 (MQ=255)                                                                                         aTCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATg                                                                                        <  1:1173241/90‑1 (MQ=255)                                                                                         aTCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt                                       <  1:1190154/139‑1 (MQ=255)                                                                                                 aaaGAACATCATGATGTACATGATGATTTCGTTAATTCCCCGTCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAcgcg                               >  1:476711/1‑139 (MQ=255)                                                                                                   aGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAgttgt                                                                                                        <  1:1392827/64‑1 (MQ=255)                                                                                                   aGAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAgttgt                                                                                                        >  2:1392827/1‑64 (MQ=255)                                                                                                    gAACATCATGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa                            <  1:1364628/139‑1 (MQ=255)                                                                                                        atcatGATGTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATccc                                                  >  1:386683/1‑113 (MQ=255)                                                                                                        atcatGATGTACATGATGATTTCGTTAATT‑ccccat                                                                                                                              <  1:1137622/36‑4 (MQ=255)                                                                                                        atcatGATGTACATGATGATTTCGTTAATT‑ccccat                                                                                                                              >  2:1137622/1‑33 (MQ=255)                                                                                                        atcatGATGTACATGATGAGTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATccc                                                  <  2:386683/113‑1 (MQ=255)                                                                                                             gatgTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa                   >  1:593129/1‑139 (MQ=255)                                                                                                             gatgTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa                   >  2:271777/1‑139 (MQ=255)                                                                                                               tgTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAgt                                                                                                           <  2:879237/49‑1 (MQ=39)                                                                                                               tgTACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAgt                                                                                                           >  1:879237/1‑49 (MQ=39)                                                                                                                 tACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATgcg                                                                                      >  2:1353326/1‑68 (MQ=255)                                                                                                                 tACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATgcg                                                                                      <  1:1353326/68‑1 (MQ=255)                                                                                                                 tACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCt                                                                <  2:55454/90‑1 (MQ=255)                                                                                                                 tACATGATGATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCt                                                                >  1:55454/1‑90 (MQ=255)                                                                                                                    atgatgATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATc                                                                      >  2:1181538/1‑81 (MQ=255)                                                                                                                    atgatgATTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATc                                                                      <  1:1181538/81‑1 (MQ=255)                                                                                                                       atgatTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAtt         >  2:673980/1‑139 (MQ=255)                                                                                                                         gatTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATgcg                                                                                      <  1:1504005/60‑1 (MQ=255)                                                                                                                         gatTTCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATgcg                                                                                      >  2:1504005/1‑60 (MQ=255)                                                                                                                           tttCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATc                                                                      >  2:274132/1‑74 (MQ=255)                                                                                                                           tttCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATc                                                                      <  1:274132/74‑1 (MQ=255)                                                                                                                           tttCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg                                             <  2:953823/99‑1 (MQ=255)                                                                                                                           tttCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc     <  1:1270984/139‑1 (MQ=255)                                                                                                                           tttCGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCATTATCCGCCCTACATGTGCAATCCCGTAgg                                             >  1:953823/1‑99 (MQ=255)                                                                                                                              cGTTAATTCCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc  >  2:1469026/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                   ACCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑CCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGC  >  NC_000913/2567970‑2568232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑