Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,138	+C	coding (384/498 nt)	yggI →	Zn‑dependent metalloprotease‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,134	1	.	C	100.0%	192.8 / NA	54	F127S (TTC→TCC)	yggI	Zn‑dependent metalloprotease‑related protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (31/23);  total (31/23)

TGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTT‑CCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATC  >  NC_000913/3090010‑3090266                                                                                                                              |                                                                                                                                     tGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGc                                                                                                                         >  2:215638/1‑139 (MQ=255)         tttCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCa                                                                                                                 >  1:981148/1‑139 (MQ=255)               ccGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCa                                                                                                           <  2:445547/139‑1 (MQ=255)                  cgTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCa                                                                                                        >  1:976214/1‑139 (MQ=255)                    tAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCa                                                                                                                        >  2:1304059/1‑121 (MQ=255)                    tAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCa                                                                                                                        <  1:1304059/121‑1 (MQ=255)                               ggCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTAcgc                                                                                           <  1:378908/139‑1 (MQ=255)                                      agtggaagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCa                                                                                                        >  2:463471/1‑119 (MQ=255)                                      agtggaagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCa                                                                                                        <  1:463471/119‑1 (MQ=255)                                           aagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATcgc                                                                               >  1:313047/1‑139 (MQ=255)                                                gatgatGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCa                                                                                                           >  2:1095532/1‑106 (MQ=255)                                                gatgatGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCa                                                                                                           <  1:1095532/106‑1 (MQ=255)                                                gatgatGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGt                                                                          >  1:19778/1‑139 (MQ=255)                                                gatgatGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCATACGCCGCCATAATCGCGTAGt                                                                          >  1:1109909/1‑139 (MQ=255)                                                   gatgGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCg                                                                       <  2:313047/139‑1 (MQ=255)                                                     tgGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCAt                                                                                                          >  1:1351851/1‑102 (MQ=255)                                                     tgGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCAt                                                                                                          <  2:1351851/102‑1 (MQ=255)                                                     tgGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTg                                                                     >  2:1108657/1‑139 (MQ=255)                                                     tgGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTg                                                                     >  1:490874/1‑139 (MQ=255)                                                     tgGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTg                                                                     >  1:661900/1‑139 (MQ=255)                                                        aaaaCGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCg                                                                  >  1:1309048/1‑139 (MQ=255)                                                           aCGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAgg                                                               <  2:19778/139‑1 (MQ=255)                                                             gTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGcc                                                             <  2:1309048/139‑1 (MQ=255)                                                             gTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGcc                                                             <  2:976214/139‑1 (MQ=255)                                                               gCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCa                                                                                                           <  1:762181/91‑1 (MQ=255)                                                               gCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCa                                                                                                           >  2:762181/1‑91 (MQ=255)                                                                cTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTc                                                          >  2:1179863/1‑139 (MQ=255)                                                                       ttCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTT‑ccccctac                                                                                                                              <  2:938677/63‑5 (MQ=255)                                                                       ttCCCGCCCGTCGTACGCATCAGTACGAACTGCAATCCGTGCGTCGCAACATCTT‑ccccctac                                                                                                                              >  1:938677/1‑59 (MQ=255)                                                                        tCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCtg                                                  >  1:315718/1‑139 (MQ=255)                                                                             cccgTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTc                                             >  2:736782/1‑139 (MQ=255)                                                                               cgtcgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCAc                                           >  1:843865/1‑139 (MQ=255)                                                                                 tcgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTAc                                                                                             <  1:980720/87‑1 (MQ=255)                                                                                 tcgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTAc                                                                                             >  2:980720/1‑87 (MQ=255)                                                                                 tcgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGc                                                              >  2:1064075/1‑118 (MQ=255)                                                                                 tcgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGc                                                              <  1:1064075/118‑1 (MQ=255)                                                                                   gtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCg                                       <  2:1508524/139‑1 (MQ=255)                                                                                            aGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCtgt                                                 >  1:251551/1‑120 (MQ=255)                                                                                            aGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCtgt                                                 <  2:251551/120‑1 (MQ=255)                                                                                                 gAACTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGtt                         <  2:843865/139‑1 (MQ=255)                                                                                                  aaCTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTg                        <  2:490874/139‑1 (MQ=255)                                                                                                  aaCTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTg                        <  2:661900/139‑1 (MQ=255)                                                                                                   aCTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGtt                                              >  2:256599/1‑116 (MQ=255)                                                                                                   aCTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGtt                                              <  1:256599/116‑1 (MQ=255)                                                                                                   aCTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGc                       >  2:436778/1‑139 (MQ=255)                                                                                                   aCTGCAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGc                       >  1:391593/1‑139 (MQ=255)                                                                                                       cAATCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGaaa                   >  1:848644/1‑139 (MQ=255)                                                                                                        aaTCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAAt                  >  2:33120/1‑139 (MQ=255)                                                                                                         aTCCGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATa                 <  1:215638/139‑1 (MQ=255)                                                                                                            cGTGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAAcc              >  2:634610/1‑139 (MQ=255)                                                                                                              tGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGcc                                                             <  2:1081299/90‑1 (MQ=255)                                                                                                              tGCGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGcc                                                             >  1:1081299/1‑90 (MQ=255)                                                                                                                cGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTc                                                          >  1:434566/1‑91 (MQ=255)                                                                                                                cGTCGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTc                                                          <  2:434566/91‑1 (MQ=255)                                                                                                                   cGCAACACCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGaa       <  1:634610/139‑1 (MQ=255)                                                                                                                        cacCTTCCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATc  >  1:1299869/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                     TGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTT‑CCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATC  >  NC_000913/3090010‑3090266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑