Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,432,994 (C)6→7 coding (567/1125 nt) smf ← DNA recombination‑mediator A family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,432,9881.C98.5% 228.6 / ‑3.9 65coding (573/1125 nt)smfDNA recombination‑mediator A family protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (35/29);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGC  >  NC_000913/3432850‑3433105
                                                                                                                                           |                                                                                                                      
tGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg                                                                                                                         >  2:122792/1‑139 (MQ=255)
 gCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcgc                                                                                                                       <  2:1278574/139‑1 (MQ=255)
 gCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcgc                                                                                                                       <  1:92091/139‑1 (MQ=255)
      cACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGcccccc                                                                                                                 >  1:532101/1‑139 (MQ=255)
          aGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGccccccctgt                                                                                                             <  1:1150391/139‑5 (MQ=255)
          aGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGcccccc                                                                                                                 <  1:1172429/135‑1 (MQ=255)
          aGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGcccccc                                                                                                                 >  2:1172429/1‑135 (MQ=255)
               aCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAg                                                                                                         >  2:978669/1‑139 (MQ=255)
               aCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAg                                                                                                         >  2:40228/1‑139 (MQ=255)
                   cTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGa                                                                                                     >  2:677721/1‑139 (MQ=255)
                     aCGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGccccc                                                                                                                  >  2:1251376/1‑123 (MQ=255)
                     aCGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGccccc                                                                                                                  <  1:1251376/123‑1 (MQ=255)
                         aaaGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCa                                                                                               >  1:400777/1‑139 (MQ=255)
                           aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATGGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACt                                                                                                   >  1:1015803/1‑133 (MQ=255)
                           aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGCGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACt                                                                                                   <  2:1015803/133‑1 (MQ=255)
                            gCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGcc                                                                                            <  1:677721/139‑1 (MQ=255)
                             ccGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCa                                                                                           >  1:1261948/1‑139 (MQ=255)
                               gCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGt                                                                                         <  1:122792/139‑1 (MQ=255)
                                cTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTc                                                                                        >  1:1134565/1‑139 (MQ=255)
                                     accaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGa                                                                                                     >  2:5072/1‑121 (MQ=255)
                                     accaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGa                                                                                                     <  1:5072/121‑1 (MQ=255)
                                           aGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg                                                                             <  2:337943/139‑1 (MQ=255)
                                             tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg                                                                           >  1:308254/1‑139 (MQ=255)
                                             tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg                                                                           >  1:741880/1‑139 (MQ=255)
                                                 ccTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGa                                                                       >  2:243284/1‑139 (MQ=255)
                                                  cTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt                                                                      >  1:436032/1‑139 (MQ=255)
                                                  cTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt                                                                      <  2:532101/139‑1 (MQ=255)
                                                      aCTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                  <  2:1503404/139‑1 (MQ=255)
                                                      aCTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                  <  2:1261948/139‑1 (MQ=255)
                                                       cTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATg                                                                 <  1:40228/139‑1 (MQ=255)
                                                         tAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcgc                                                                                                                       >  2:1415080/1‑83 (MQ=255)
                                                         tAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcgc                                                                                                                       <  1:1415080/83‑1 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGc                                                                                             <  1:1087528/106‑1 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTAtt                                                            >  2:1297443/1‑139 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTAtt                                                            >  2:1439242/1‑139 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGcccccc                                                                                                                 >  1:654116/1‑85 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGcccccc                                                                                                                 <  2:654116/85‑1 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGATGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGc                                                                                             >  2:1087528/1‑106 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAAATGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCGAGTCGGGCATGACGGCGGGGATGAATTGTaat                                                            >  2:500365/1‑137 (MQ=255)
                                                              cACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTaa                                                          <  2:741880/139‑1 (MQ=255)
                                                                   aTAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  1:1439242/139‑1 (MQ=255)
                                                                   aTAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  1:243284/139‑1 (MQ=255)
                                                                     aaTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCAtt                                                   >  2:68190/1‑139 (MQ=255)
                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAg                                                                                          <  2:539567/94‑1 (MQ=255)
                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAg                                                                                          >  1:539567/1‑94 (MQ=255)
                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                  <  2:479523/118‑1 (MQ=255)
                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                  >  1:479523/1‑118 (MQ=255)
                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     >  2:722765/1‑131 (MQ=255)
                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  1:722765/131‑1 (MQ=255)
                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                <  1:207741/136‑1 (MQ=255)
                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                >  2:207741/1‑136 (MQ=255)
                                                                           aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa                                             <  1:932161/139‑1 (MQ=255)
                                                                                 tcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGc                                       >  1:1418236/1‑139 (MQ=255)
                                                                                       gAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCt                                 <  2:400777/139‑1 (MQ=255)
                                                                                         aaTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGa                               <  2:1418236/139‑1 (MQ=255)
                                                                                          aTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGAc                              >  2:1242538/1‑139 (MQ=255)
                                                                                           ttGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg                             <  1:500365/139‑1 (MQ=255)
                                                                                              tAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCa                          <  2:436032/139‑1 (MQ=255)
                                                                                                aGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                                   >  1:1246288/1‑96 (MQ=255)
                                                                                                aGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                                   <  2:1246288/96‑1 (MQ=255)
                                                                                                    aGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTAcc                    >  1:485576/1‑139 (MQ=255)
                                                                                                      gggTGGAACATCGAGGGGAAATTCCGAGACGAGAgcgc                                                                                                                       >  2:797265/1‑38 (MQ=255)
                                                                                                      gggTGGAACATCGAGGGGAAATTCCGAGACGAGAgcgc                                                                                                                       <  1:797265/38‑1 (MQ=255)
                                                                                                                tCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  2:606670/94‑1 (MQ=255)
                                                                                                                tCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     >  1:606670/1‑94 (MQ=255)
                                                                                                                tCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATCAAGTCCATTCCCCAATACAGCAATGCTGACg                             >  1:98396/1‑118 (MQ=255)
                                                                                                                tCGAGGGGAAATTCCGAGACGAGAGCGCCCCACCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg                             <  2:98396/118‑1 (MQ=255)
                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     <  1:687754/93‑1 (MQ=255)
                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                     >  2:687754/1‑93 (MQ=255)
                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTa                <  2:404102/130‑1 (MQ=255)
                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTa                >  1:404102/1‑130 (MQ=255)
                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCtt       >  1:510891/1‑139 (MQ=255)
                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCtt       >  2:310979/1‑139 (MQ=255)
                                                                                                                  gAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCttt      >  2:253330/1‑139 (MQ=255)
                                                                                                                   aGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                  >  2:1169183/1‑94 (MQ=255)
                                                                                                                   aGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                  <  1:1169183/94‑1 (MQ=255)
                                                                                                                   aGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTa     >  1:154596/1‑139 (MQ=255)
                                                                                                                      ggAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATgc  <  2:154596/139‑1 (MQ=255)
                                                                                                                            tCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAttt                       >  2:40226/1‑112 (MQ=255)
                                                                                                                            tCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAttt                       <  1:40226/112‑1 (MQ=255)
                                                                                                                                           |                                                                                                                      
TGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGC  >  NC_000913/3432850‑3433105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: