Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,265,001 1086 bp→GAGG [prfA] [prfA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1265001 1266086 1086 45 [0] [0] 40 [prfA] [prfA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12650000 (0.000)39 (0.680)
+GAGG
33/198 0.6 100% intergenic (+30/‑12) hemA/prfA glutamyl tRNA reductase/peptide chain release factor RF‑1
?NC_000913 1266087 = 0 (0.000)coding (1076/1083 nt) prfA peptide chain release factor RF‑1

GACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1264865‑1265000
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTG  >  NC_000913/1266087‑1266223
                                                                                                                                        ||||                                                                                                                                         
GACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAG                                                                                                                                            >  2:960901/1‑139
 ACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGG                                                                                                                                           <  1:1477913/139‑1
                 ATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATAT                                                                                                                           <  2:1373441/139‑1
                   GCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGG                                                                                                                                <  2:315733/132‑1
                   GCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGG                                                                                                                                >  1:315733/1‑132
                        AACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATC                                                                                                                          <  1:572847/133‑1
                        AACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATC                                                                                                                          >  2:572847/1‑133
                        AACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGG                                                                                                                                >  1:1303522/1‑127
                        AACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGG                                                                                                                                <  2:1303522/127‑1
                        AACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGG                                                                                                                                              >  1:109730/1‑113
                        AACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGG                                                                                                                                              <  2:109730/113‑1
                                   TTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATC                                                                                                                          >  1:1492663/1‑122
                                   TTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATC                                                                                                                          <  2:1492663/122‑1
                                    TCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGG                                                                                                                  >  2:714306/1‑129
                                    TCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGG                                                                                                                  <  1:714306/129‑1
                                             CGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCC                                                                                               >  1:135843/1‑139
                                             CGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCC                                                                                               <  1:960901/139‑1
                                                     ACGGGGATAACGAACGCCTGAATATTCTGCGCGATAGCCTCGGGCTGGAGGAACCGTACATCATTTTCTTTTTTTACAGGGTGGAGGGGGAATAATGGAATATCAACAATGATTACGTGAAGCCATAAGCCAACTTCAG                                                                                       >  2:781548/1‑139
                                                          GATAACGAACGCCTGAATATTCTGGGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAG                                                                                  >  2:1040858/1‑139
                                                                   CGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAA                                                                                                                                      <  2:501583/78‑1
                                                                   CGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAA                                                                                                                                      >  1:501583/1‑78
                                                                              TCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAAT                                                                                                                                     >  1:617793/1‑68
                                                                              TCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAAT                                                                                                                                     <  2:617793/68‑1
                                                                                TGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCT                                                            >  2:415226/1‑139
                                                                                TGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCT                                                            <  2:135843/139‑1
                                                                                  CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACA                                                                                                                      <  2:1412152/79‑1
                                                                                  CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACA                                                                                                                      >  1:1412152/1‑79
                                                                                  CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAAC                                                                                                                       >  2:248358/1‑78
                                                                                  CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAAC                                                                                                                       <  1:248358/78‑1
                                                                                          CCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGC                                                  <  1:1040858/139‑1
                                                                                                    GAGTAACAGTACATGATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGT                                                                  <  1:1028106/113‑1
                                                                                                    GAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGT                                                                  >  2:1028106/1‑113
                                                                                                        aaCAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACC                                    >  1:81521/3‑139
                                                                                                          CAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAA                                                                              >  2:1037687/1‑95
                                                                                                          CAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAA                                                                              <  1:1037687/95‑1
                                                                                                              ACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATG                                                                                                                                 >  2:1032280/1‑40
                                                                                                              ACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATG                                                                                                                                 <  1:1032280/40‑1
                                                                                                               CATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAG                             >  2:1076823/1‑139
                                                                                                                   ATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCG                         <  1:415226/139‑1
                                                                                                                     TTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCG                         >  2:387877/1‑137
                                                                                                                     TTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCG                         <  1:387877/137‑1
                                                                                                                       TCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGC                                                                                     >  1:670989/1‑75
                                                                                                                       TCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGC                                                                                     <  2:670989/75‑1
                                                                                                                                          GGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTG  <  1:1076823/139‑1
                                                                                                                                        ||||                                                                                                                                         
GACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1264865‑1265000
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTG  >  NC_000913/1266087‑1266223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.