Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,857,1430TA48.6% 1.8 / 53.7 35Y17* (TAT→TAAmutSmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base T (8/10);  new base A (11/6);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTATCTCAGGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATAT  >  NC_000913/2857040‑2857250
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gAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCctgct                                                                          <  2:449981/139‑1 (MQ=255)
         aaCCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATcc                                                                                      <  2:291536/118‑1 (MQ=255)
         aaCCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATcc                                                                                      >  1:291536/1‑118 (MQ=255)
              tCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATgg                                                            >  2:1262184/1‑139 (MQ=255)
               cacaCCCCATTTAATATCAGGCAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATggg                                                           >  2:586429/1‑139 (MQ=255)
                      cATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAGGCCCAGCATCCCGAGATCctg                                                                            >  1:145734/1‑115 (MQ=255)
                      cATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAGGCCCAGCATCCCGAGATCctg                                                                            <  2:145734/115‑1 (MQ=255)
                      cATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGAtttt                                                    >  1:35122/1‑139 (MQ=255)
                            atatCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa                                              <  2:35122/139‑1 (MQ=255)
                              atCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACt                                            >  2:1422080/1‑139 (MQ=255)
                              atCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGAGGCAGTAGTGATAGTCGCTGAAAGTCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACt                                            >  1:977391/1‑139 (MQ=255)
                                cAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGt                                          >  2:162590/1‑139 (MQ=255)
                                  gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGttt                                        >  1:1478260/1‑139 (MQ=255)
                                  gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATCGGTGATTTTTATGAACTGttt                                        >  1:902066/1‑139 (MQ=255)
                                  gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGttt                                        >  1:1510564/1‑139 (MQ=255)
                                  gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGttt                                        <  2:1158303/139‑1 (MQ=255)
                                   ggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGtttt                                       >  1:1219638/1‑139 (MQ=255)
                                       ccGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTAcc                                                                  <  1:226526/108‑1 (MQ=255)
                                       ccGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTAcc                                                                  >  2:226526/1‑108 (MQ=255)
                                          gACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATgacga                                <  2:1219638/139‑1 (MQ=255)
                                                    cATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAAcgc                      >  1:741650/1‑139 (MQ=255)
                                                     aTGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAAcgcg                     >  1:402806/1‑139 (MQ=255)
                                                     aTGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAAcgcg                     >  1:1099006/1‑139 (MQ=255)
                                                     aTGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAAcgcg                     <  1:586429/139‑1 (MQ=255)
                                                     aTGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAAc                                             >  1:167075/1‑115 (MQ=255)
                                                     aTGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAAc                                             <  2:167075/115‑1 (MQ=255)
                                                         gTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCa                                                                                         <  1:1118918/67‑1 (MQ=255)
                                                         gTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCa                                                                                         >  2:1118918/1‑67 (MQ=255)
                                                          tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCctgctg                                                                         <  1:323230/82‑1 (MQ=255)
                                                          tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCctgctg                                                                         >  2:323230/1‑82 (MQ=255)
                                                           gCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGc               <  1:1422080/139‑1 (MQ=255)
                                                            cAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCa              <  1:162590/139‑1 (MQ=255)
                                                                aGAAAATTTCGACGCCCATACGCCCACGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAActg          <  2:977391/139‑1 (MQ=255)
                                                                  aaaaTTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCTAAACGCGCGTCGCAActgct        <  2:902066/139‑1 (MQ=255)
                                                                        tCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGatat  <  2:402806/139‑1 (MQ=255)
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GAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTATCTCAGGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATAT  >  NC_000913/2857040‑2857250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: