Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	551,332	G→A	*298* (TAG→TAA)	ybcF →	putative carbonate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	551,332	0	G	A	100.0%	167.7 / NA	53	*298* (TAG→TAA)	ybcF	putative carbonate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/28);  total (25/28)

GATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAT  >  NC_000913/551198‑551463                                                                                                                                       |                                                                                                                                    gATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGt                                                                                                                                 <  1:1473615/139‑1 (MQ=255)  aTGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTa                                                                                                                                <  2:1278747/139‑1 (MQ=255)    ggTTCGATGGGGCCGAATGTAACGGCGGTGAGCGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAG‑GACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGc                                                                                                                             >  1:377295/1‑139 (MQ=255)          aTGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAg                                                                                                                        <  2:1235633/139‑1 (MQ=255)          aTGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAg                                                                                                                        <  1:451536/139‑1 (MQ=255)                ccGAATGTAACGGCGGTGAGCGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTAGCTGTAATCGTAAGCATTAGACAttt                                                                                                                  >  2:1256416/1‑139 (MQ=255)                          cggcggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCTTGTATTGGGGCGTTATCGCGAATTGAAG‑GACGCTGGCGGGCGATGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGc                                                                                                       <  2:377295/139‑1 (MQ=255)                             cggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGa                                                                                                     >  1:347181/1‑139 (MQ=255)                               gTGAGTGCTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGACGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGAGATTTGTGCCTGATGCGACg                                                                                                   >  2:1005491/1‑139 (MQ=255)                                       ttATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACAtt                                                                                                                   >  1:1233460/1‑115 (MQ=255)                                       ttATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACAtt                                                                                                                   <  2:1233460/115‑1 (MQ=255)                                        tATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGAcgcg                                                                                          <  1:639745/139‑1 (MQ=255)                                            tCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCtt                                                                                      >  2:1072330/1‑139 (MQ=255)                                            tCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCtt                                                                                      >  1:356505/1‑139 (MQ=255)                                              aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                    >  1:1146563/1‑139 (MQ=255)                                                aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCa                                                                                  >  1:1449381/1‑139 (MQ=255)                                                aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCa                                                                                  <  2:347181/139‑1 (MQ=255)                                                 aGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                 >  1:1371998/1‑139 (MQ=255)                                                      tGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGGCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTa                                                                            >  1:1015347/1‑139 (MQ=255)                                                      tGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTa                                                                            >  2:695169/1‑139 (MQ=255)                                                          aaaCCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAc                                                                        <  2:1371998/139‑1 (MQ=255)                                                             cccGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCgg                                                                     >  1:19847/1‑139 (MQ=255)                                                                 cgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAg                                                                                                                        <  2:813531/84‑1 (MQ=255)                                                                 cgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAg                                                                                                                        >  1:813531/1‑84 (MQ=255)                                                                        tGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGcc                                                                                                                            <  1:943714/73‑2 (MQ=255)                                                                        tGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCa                                                                                                                            >  2:943714/1‑73 (MQ=255)                                                                           ggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAgg                                                                                                                              <  2:395241/68‑1 (MQ=255)                                                                           ggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAgg                                                                                                                              >  1:395241/1‑68 (MQ=255)                                                                               ttATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTTTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTg                                                   >  2:1097408/1‑139 (MQ=255)                                                                                     cgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac                                             <  1:688287/139‑1 (MQ=255)                                                                                     cgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac                                             <  2:1449381/139‑1 (MQ=255)                                                                                                gaCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatg                                  <  1:695169/139‑1 (MQ=255)                                                                                                 aCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAg                                                                                                                        >  2:663123/1‑52 (MQ=255)                                                                                                 aCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAg                                                                                                                        <  1:663123/52‑1 (MQ=255)                                                                                                      ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCa                                                                                                                            <  1:22473/43‑1 (MQ=255)                                                                                                      ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCa                                                                                                                            >  2:22473/1‑43 (MQ=255)                                                                                                      ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcgatgc                            >  2:42755/1‑139 (MQ=255)                                                                                                                    ggACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt              <  1:1005491/139‑1 (MQ=255)                                                                                                                    ggACCTGTATTTCGCTGTAATCGTAGCCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGGGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt              <  2:156144/139‑1 (MQ=255)                                                                                                                         tGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                 >  2:330595/1‑67 (MQ=255)                                                                                                                         tGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                 <  1:330595/67‑1 (MQ=255)                                                                                                                         tGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGc         <  1:11085/139‑1 (MQ=255)                                                                                                                           tATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc                                                                           <  2:630693/71‑1 (MQ=255)                                                                                                                           tATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc                                                                           >  1:630693/1‑71 (MQ=255)                                                                                                                                cGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATg                                                                                                        <  1:655006/37‑1 (MQ=255)                                                                                                                                cGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATg                                                                                                        >  2:655006/1‑37 (MQ=255)                                                                                                                                cGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatg                                  >  2:202385/1‑107 (MQ=255)                                                                                                                                cGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatg                                  <  1:202385/107‑1 (MQ=255)                                                                                                                                cGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt  <  2:19847/139‑1 (MQ=255)                                                                                                                                cGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt  <  1:42755/139‑1 (MQ=255)                                                                                                                                cGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt  <  1:1072330/139‑1 (MQ=255)                                                                                                                                  cTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt              >  1:131761/1‑125 (MQ=255)                                                                                                                                  cTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt              <  2:131761/125‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    GATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAT  >  NC_000913/551198‑551463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑