Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 649,648 A→G G281G (GGT→GGC)  citE ← citrate lyase, citryl‑ACP lyase (beta) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913649,6480AG100.0% 127.0 / NA 38G281G (GGT→GGC) citEcitrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

ACGATTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAG  >  NC_000913/649515‑649773
                                                                                                                                     |                                                                                                                             
acgaTTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTc                                                                                                                          >  1:637610/1‑139 (MQ=255)
                                gagaTTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGa                                                                                          >  2:1414853/1‑139 (MQ=255)
                                       ttCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTc                                                                                   >  2:434041/1‑139 (MQ=255)
                                                                  cTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAggg                                                                                                      <  2:761218/93‑1 (MQ=255)
                                                                  cTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAggg                                                                                                      >  1:761218/1‑93 (MQ=255)
                                                                           cgcgGATGGCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCAACGACgctgcg                                               >  1:997195/1‑139 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAgcgg                                                                           >  1:1013678/1‑111 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCa                                                                   >  2:426268/1‑119 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCa                                                                   <  1:426268/119‑1 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                               >  1:753981/1‑139 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                               >  1:1484614/1‑139 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                               >  1:1181874/1‑139 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGAGAACCCGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAgcgg                                                                           <  2:1013678/111‑1 (MQ=255)
                                                                               gATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCggcgggcg                                           <  2:1199679/139‑1 (MQ=255)
                                                                                        aaGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCAct                                  <  2:1181874/139‑1 (MQ=255)
                                                                                              tGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACttctttc                            >  1:109930/1‑139 (MQ=255)
                                                                                                   gggAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAgcg                                                                            >  1:836762/1‑86 (MQ=255)
                                                                                                   gggAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAgcg                                                                            <  2:836762/86‑1 (MQ=255)
                                                                                                   gggAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGt                       <  1:1414853/139‑1 (MQ=255)
                                                                                                    ggAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTc                      <  2:738526/139‑1 (MQ=255)
                                                                                                     gagaGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                     >  1:258733/1‑139 (MQ=255)
                                                                                                     gagaGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                     >  1:42217/1‑139 (MQ=255)
                                                                                                     gagaGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                     <  2:637610/139‑1 (MQ=255)
                                                                                                        agCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAgcggc                                                                          >  2:1199227/1‑83 (MQ=255)
                                                                                                        agCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAgcggc                                                                          <  1:1199227/83‑1 (MQ=255)
                                                                                                          cACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCATTTCTTTCTGGGTCg                     <  1:239687/134‑1 (MQ=255)
                                                                                                          cACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                     >  2:239687/1‑134 (MQ=255)
                                                                                                           aCCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGt               <  1:1141234/139‑1 (MQ=255)
                                                                                                             cAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                     <  1:108784/131‑1 (MQ=255)
                                                                                                             cAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                     >  2:108784/1‑131 (MQ=255)
                                                                                                               gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGc                                                                 >  1:1001856/1‑85 (MQ=255)
                                                                                                               gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGc                                                                 <  2:1001856/85‑1 (MQ=255)
                                                                                                               gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTagag           >  2:1109076/1‑139 (MQ=255)
                                                                                                                     cgcgATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                     >  2:1327044/1‑123 (MQ=255)
                                                                                                                     cgcgATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                     <  1:1327044/123‑1 (MQ=255)
                                                                                                                      gcgATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGcggcgg                                              >  2:1463142/1‑97 (MQ=255)
                                                                                                                      gcgATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGcggcgg                                              <  1:1463142/97‑1 (MQ=255)
                                                                                                                        gatcgatAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTgcag  <  1:1109076/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                             
ACGATTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAG  >  NC_000913/649515‑649773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑