| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 672,947 | C→A | V613F (GTT→TTT) | leuS ← | leucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 672,947 | 0 | C | A | 96.3% | 167.6 / ‑0.5 | 54 | coding (1837/2583 nt) | leuS | leucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (29/23); minor base . (1/1); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
TCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAC > NC_000913/672821‑673079 | tCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGcc > 2:1101378/1‑139 (MQ=255) atatCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTg > 2:1157036/1‑139 (MQ=255) ccGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGGCGCATc > 1:378315/1‑139 (MQ=255) cGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCt > 1:1075355/1‑139 (MQ=255) atcatAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGa < 1:1157036/139‑1 (MQ=255) tAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACg > 2:37893/1‑139 (MQ=255) aGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGa < 1:916265/130‑1 (MQ=255) aGACGAACGGTGTCCGCGCCGTAACGTCCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGa > 2:916265/1‑130 (MQ=255) gACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCttt > 1:1089954/1‑139 (MQ=255) gACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCttt > 1:423940/1‑139 (MQ=255) gACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCttt > 2:1473315/1‑139 (MQ=255) gACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCttt > 1:1438687/1‑139 (MQ=255) gACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCttt > 2:637009/1‑139 (MQ=255) aCGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTtc > 2:489766/1‑139 (MQ=255) cGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAA‑CAGTTCATgg > 2:650679/1‑101 (MQ=255) cGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAA‑CAGTTCATgg < 1:650679/101‑1 (MQ=255) aCGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGt > 2:816675/1‑139 (MQ=255) aCGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGt < 1:816675/139‑1 (MQ=255) ggTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa < 1:489766/139‑1 (MQ=255) ggTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa < 1:1101378/139‑1 (MQ=255) tCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGtt < 1:495766/139‑1 (MQ=255) tCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGtt > 1:1110284/1‑139 (MQ=255) tCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTACCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGtt > 2:623663/1‑139 (MQ=255) gcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACg < 1:1473315/139‑1 (MQ=255) tAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAg > 1:961832/1‑139 (MQ=255) aaCGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGc > 2:1237989/1‑139 (MQ=255) aCCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaaa < 2:1075355/139‑1 (MQ=255) cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGc > 1:926707/1‑65 (MQ=255) cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGc < 2:926707/65‑1 (MQ=255) cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCATTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAg > 2:906456/1‑139 (MQ=255) ggTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGtt > 2:191590/1‑139 (MQ=255) gTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTa > 1:1481063/1‑139 (MQ=255) aCCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCg < 2:1110284/139‑1 (MQ=255) aCCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCg < 2:1481063/139‑1 (MQ=255) ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCttt > 1:629310/1‑65 (MQ=255) ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCttt < 2:629310/65‑1 (MQ=255) tcttcGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTc > 1:857774/1‑139 (MQ=255) cttcGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCg > 1:337615/1‑139 (MQ=255) ttcGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc < 2:1089954/139‑1 (MQ=255) gACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCaa < 2:337615/139‑1 (MQ=255) gACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCaa < 2:961832/139‑1 (MQ=255) tGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc < 2:250337/100‑1 (MQ=255) tGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc > 1:250337/1‑100 (MQ=255) ggACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATAc < 2:1056652/65‑1 (MQ=255) ggACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATAc > 1:1056652/1‑65 (MQ=255) gACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCAt > 2:1437266/1‑46 (MQ=255) gACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCAt < 1:1437266/46‑1 (MQ=255) gCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa < 1:906456/139‑1 (MQ=255) tCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATc > 1:1130652/1‑139 (MQ=255) tGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGc < 1:37893/139‑1 (MQ=255) ccGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCa < 1:191590/139‑1 (MQ=255) ggTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCAt > 2:1219022/1‑131 (MQ=255) ggTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCAt < 1:1219022/131‑1 (MQ=255) gTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCAGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCa < 1:1237989/139‑1 (MQ=255) aaaaCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAc < 2:609183/136‑1 (MQ=255) | TCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAC > NC_000913/672821‑673079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |