Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	677,889	G→A	A159T (GCC→ACC)	djlB →	putative HscC co‑chaperone, uncharacterized J domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	677,889	0	G	A	100.0%	134.5 / NA	42	A159T (GCC→ACC)	djlB	putative HscC co‑chaperone, uncharacterized J domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/20);  total (22/20)

TGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTG  >  NC_000913/677760‑678014                                                                                                                                  |                                                                                                                              tgCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGa                                                                                                                      <  2:843958/139‑1 (MQ=255)       aTCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                <  1:700267/139‑1 (MQ=255)                            ttATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                        <  1:765232/126‑1 (MQ=255)                            ttATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                        >  2:765232/1‑126 (MQ=255)                                          cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGccc                                                                             >  1:619705/1‑139 (MQ=255)                                          cGTGGTATTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGccc                                                                             >  2:657889/1‑139 (MQ=255)                                                         aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGCACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                >  2:756539/1‑89 (MQ=255)                                                         aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCa                                                                                                                            <  2:1479501/77‑1 (MQ=255)                                                         aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCa                                                                                                                            >  1:1479501/1‑77 (MQ=255)                                                         aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                <  1:756539/89‑1 (MQ=255)                                                         aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAAt                                                              >  2:2528/1‑139 (MQ=255)                                                         aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATACTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAAt                                                              >  1:911155/1‑139 (MQ=255)                                                         aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAGTGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATg                                                             >  1:1240840/1‑139 (MQ=255)                                                                  gCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCaaa                                                                <  1:153092/128‑1 (MQ=255)                                                                  gCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCaaa                                                                >  2:153092/1‑128 (MQ=255)                                                                  gCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATc                                                     >  1:1118288/1‑139 (MQ=255)                                                                     gtggtTACAGGAGGAGAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAAGTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAt                                                  >  2:1025129/1‑139 (MQ=255)                                                                     gtggtTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAt                                                  >  2:173800/1‑139 (MQ=255)                                                                                  gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                     >  2:672000/1‑139 (MQ=255)                                                                                         ccGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCg                              >  2:84994/1‑139 (MQ=255)                                                                                                 aTAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGt                      >  2:1336822/1‑139 (MQ=255)                                                                                                    gACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGTTTTTTACCAAATGTTCGCTCGGATTTGGTCAt                   <  2:911155/139‑1 (MQ=255)                                                                                                    gACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCAt                   <  1:2528/139‑1 (MQ=255)                                                                                                       gaagaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCa                                                       <  1:1078672/100‑1 (MQ=255)                                                                                                       gaagaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCa                                                       >  2:1078672/1‑100 (MQ=255)                                                                                                       gaagaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGc                >  2:113564/1‑139 (MQ=255)                                                                                                        aagaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCa               <  1:1336822/139‑1 (MQ=255)                                                                                                        aagaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCa               <  1:672000/139‑1 (MQ=255)                                                                                                        aagaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCa               >  1:45035/1‑139 (MQ=255)                                                                                                          gaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGa                                                            <  2:1255430/92‑1 (MQ=255)                                                                                                          gaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGa                                                            >  1:1255430/1‑92 (MQ=255)                                                                                                              aaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCcaca         <  2:45035/139‑1 (MQ=255)                                                                                                               aCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAtttt                                               <  1:104157/100‑1 (MQ=255)                                                                                                               aCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAtttt                                               >  2:104157/1‑100 (MQ=255)                                                                                                                   gAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGc    <  1:1025129/139‑1 (MQ=255)                                                                                                                   gAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGc    <  1:84994/139‑1 (MQ=255)                                                                                                                   gAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGc    <  1:657889/139‑1 (MQ=255)                                                                                                                   gAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGc    <  1:173800/139‑1 (MQ=255)                                                                                                                     aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCTAAATGACACCATCGAtt                                                 <  1:960789/92‑1 (MQ=255)                                                                                                                     aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAtt                                                 >  2:960789/1‑92 (MQ=255)                                                                                                                        cTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGAATTTGGTCATCGCATCCACAATGGCTg  >  2:156353/1‑136 (MQ=255)                                                                                                                        cTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGAATTTGGTCATCGCATCCACAATGGCTg  <  1:156353/136‑1 (MQ=255)                                                                                                                                  |                                                                                                                              TGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTG  >  NC_000913/677760‑678014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑