Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	679,874	G→A	G123R (GGG→AGG)	ybeU →	DUF1266 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	679,874	0	G	A	100.0%	216.4 / NA	67	G123R (GGG→AGG)	ybeU	DUF1266 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/35);  total (32/35)

TCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAG  >  NC_000913/679742‑680009                                                                                                                                     |                                                                                                                                        tctTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTc                                                                                                                                   <  2:1384830/139‑1 (MQ=255)           aaGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGt                                                                                                                         >  2:1280062/1‑139 (MQ=255)                tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                                    >  2:127329/1‑139 (MQ=255)                   aaaGCATTAATTGCTGGTGGCTCGAAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGc                                                                                                                 >  2:1040684/1‑139 (MQ=255)                              tGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTgg                                                                                                      <  1:127329/139‑1 (MQ=255)                                cTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCt                                                                                                    >  1:467347/1‑139 (MQ=255)                                cTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCGGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCt                                                                                                    <  1:1072093/139‑1 (MQ=255)                                 tggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCt                                                                                                    >  1:1277532/1‑138 (MQ=255)                                 tggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCt                                                                                                    <  2:1277532/138‑1 (MQ=255)                                  ggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                                  <  1:1115848/139‑1 (MQ=255)                                  ggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                                  >  2:1035481/1‑139 (MQ=255)                                                 gggTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGtt                                                                                   >  1:475051/1‑139 (MQ=255)                                                  ggTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGttt                                                                                  >  1:860177/1‑139 (MQ=255)                                                  ggTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGttt                                                                                  >  2:728/1‑139 (MQ=255)                                                               ccAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAACAGAGTTTATGGCTGCAATcg                                                                     <  1:1040684/139‑1 (MQ=255)                                                                 aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                                                   >  2:543394/1‑139 (MQ=255)                                                                 aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                                                   <  2:93030/139‑1 (MQ=255)                                                                 aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTACTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                                                   <  1:1170887/139‑1 (MQ=255)                                                                  aTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACggg                                                                                                                          <  1:994064/83‑1 (MQ=255)                                                                  aTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACggg                                                                                                                          >  2:994064/1‑83 (MQ=255)                                                                  aTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                                                  <  1:534068/139‑1 (MQ=255)                                                                  aTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                                                  <  1:1280062/139‑1 (MQ=255)                                                                  aTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                                                  >  2:368375/1‑139 (MQ=255)                                                                    tCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                <  1:212703/139‑1 (MQ=255)                                                                    tCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                <  2:230852/139‑1 (MQ=255)                                                                        tGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCt                                                                                                                >  2:1509808/1‑87 (MQ=255)                                                                        tGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCt                                                                                                                <  1:1509808/87‑1 (MQ=255)                                                                               aCCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                                     <  1:1366367/139‑1 (MQ=255)                                                                               aCCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                                     <  2:467347/139‑1 (MQ=255)                                                                                  gCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTc                                                  <  1:543394/139‑1 (MQ=255)                                                                                   cTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCa                                                 >  2:809839/1‑139 (MQ=255)                                                                                    tATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                <  1:1337343/123‑1 (MQ=255)                                                                                    tATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                >  2:1337343/1‑123 (MQ=255)                                                                                           tgtgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTc                                                  >  2:592028/1‑130 (MQ=255)                                                                                           tgtgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTc                                                  <  1:592028/130‑1 (MQ=255)                                                                                           tgtgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACt                                         >  2:539583/1‑139 (MQ=255)                                                                                                ggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATcac                                    >  2:195416/1‑139 (MQ=255)                                                                                                  aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                                  <  1:507451/75‑1 (MQ=255)                                                                                                  aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                                  >  2:507451/1‑75 (MQ=255)                                                                                                  aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                <  2:332696/109‑1 (MQ=255)                                                                                                  aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                >  1:332696/1‑109 (MQ=255)                                                                                                  aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAg                                  >  2:515254/1‑139 (MQ=255)                                                                                                    gggggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCt                                >  1:959398/1‑139 (MQ=255)                                                                                                     taagagatagaGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTAATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTg                               <  2:612179/129‑1 (MQ=255)                                                                                                       ggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAagaaga                                                                                        >  1:1533208/1‑80 (MQ=255)                                                                                                       ggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAagaaga                                                                                        <  2:1533208/80‑1 (MQ=255)                                                                                                       ggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                                     <  2:1058264/115‑1 (MQ=255)                                                                                                       ggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                                     >  1:1058264/1‑115 (MQ=255)                                                                                                         aaTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACgg                                                                                               <  2:420282/71‑1 (MQ=255)                                                                                                         aaTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACgg                                                                                               >  1:420282/1‑71 (MQ=255)                                                                                                          aTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAagaaga                                                                                        <  2:1377096/77‑1 (MQ=255)                                                                                                          aTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAagaaga                                                                                        >  1:1377096/1‑77 (MQ=255)                                                                                                                    gggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTc                <  1:539583/139‑1 (MQ=255)                                                                                                                     ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                                  >  2:1021038/1‑56 (MQ=255)                                                                                                                     ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                                  <  1:1021038/56‑1 (MQ=255)                                                                                                                     ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTcc               >  2:1384652/1‑139 (MQ=255)                                                                                                                       aTTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGc             <  1:515254/139‑1 (MQ=255)                                                                                                                        ttATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGcc            <  1:1194621/139‑1 (MQ=255)                                                                                                                          aTGTTCGAATGAGGTTTATGTCACGGGTCGGTGTACTCAACAAGTGTCTGACGGAAGAAGAGAGTTTATGCCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACAAttctggcactta          >  1:612179/1‑129 (MQ=255)                                                                                                                           tGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAagagag                                                                                     <  2:229694/63‑1 (MQ=255)                                                                                                                           tGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAagagag                                                                                     >  1:229694/1‑63 (MQ=255)                                                                                                                           tGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                           <  1:1322704/121‑1 (MQ=255)                                                                                                                           tGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                           >  2:1322704/1‑121 (MQ=255)                                                                                                                             ttCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTgg                                                                                                      <  1:1501937/44‑1 (MQ=255)                                                                                                                             ttCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTgg                                                                                                      >  2:1501937/1‑44 (MQ=255)                                                                                                                                  aTGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAg  <  2:1334876/139‑1 (MQ=255)                                                                                                                                  aTGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAg  <  1:1035481/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAG  >  NC_000913/679742‑680009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑