Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	825,102	G→A	A336V (GCG→GTG)	ybhR ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	825,102	0	G	A	100.0%	183.1 / NA	57	A336V (GCG→GTG)	ybhR	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/27);  total (30/27)

TAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCAT  >  NC_000913/824973‑825235                                                                                                                                  |                                                                                                                                      tAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCa                                                                                                                              <  1:969986/139‑1 (MQ=255)        tttGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                       >  1:1250316/1‑139 (MQ=255)              ggCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTACCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCtt                                                                                                                 >  2:1475930/1‑139 (MQ=255)                    aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAAt                                                                                                           <  2:63462/139‑1 (MQ=255)                                aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                               <  2:1250316/139‑1 (MQ=255)                                     aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                          <  2:1059716/139‑1 (MQ=255)                                      ccTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                         <  2:192796/139‑1 (MQ=255)                                         tACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGc                                                                                                                   >  2:392642/1‑110 (MQ=255)                                         tACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGc                                                                                                                   <  1:392642/110‑1 (MQ=255)                                           cGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataa                                                                                                                         >  1:1312694/1‑102 (MQ=255)                                           cGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataa                                                                                                                         <  2:1312694/102‑1 (MQ=255)                                            gTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                                                                  >  1:1441437/1‑124 (MQ=255)                                            gTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                                                                  <  2:1441437/124‑1 (MQ=255)                                                 aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc                                                                              >  2:822824/1‑139 (MQ=255)                                                 aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc                                                                              >  1:421891/1‑139 (MQ=255)                                                      cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                               >  2:1086376/1‑117 (MQ=255)                                                      cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                               <  1:1086376/117‑1 (MQ=255)                                                      cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGt                                                                                    <  1:1140428/128‑1 (MQ=255)                                                      cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGt                                                                                    >  2:1140428/1‑128 (MQ=255)                                                      cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTc                                                                         >  1:73013/1‑139 (MQ=255)                                                               cgcTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGc                                                                <  2:825658/139‑1 (MQ=255)                                                                    aCCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCa                                                           <  2:1478682/139‑1 (MQ=255)                                                                         gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                          >  1:636325/1‑103 (MQ=255)                                                                         gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                          <  2:636325/103‑1 (MQ=255)                                                                                   ttATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCa                                                                                                                              >  2:558809/1‑57 (MQ=255)                                                                                   ttATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCa                                                                                                                              <  1:558809/57‑1 (MQ=255)                                                                                   ttATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGc                                                    >  1:603589/1‑131 (MQ=255)                                                                                   ttATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGc                                                    <  2:603589/131‑1 (MQ=255)                                                                                             tAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCt                                                                                                                  <  2:20186/59‑1 (MQ=255)                                                                                             tAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCt                                                                                                                  >  1:20186/1‑59 (MQ=255)                                                                                                cGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACg                               >  1:225516/1‑139 (MQ=255)                                                                                                  gCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                    <  1:326123/68‑1 (MQ=255)                                                                                                  gCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                    >  2:326123/1‑68 (MQ=255)                                                                                                  gCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTa                             >  2:809225/1‑139 (MQ=255)                                                                                                    cacaAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAAt                                                                                                           <  1:27381/59‑1 (MQ=255)                                                                                                    cacaAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAAt                                                                                                           >  2:27381/1‑59 (MQ=255)                                                                                                             ttCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGga                  >  2:1221722/1‑139 (MQ=255)                                                                                                             ttCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGga                  >  2:693846/1‑139 (MQ=255)                                                                                                                 acacaATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                    >  2:1446532/1‑53 (MQ=255)                                                                                                                 acacaATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                    <  1:1446532/53‑1 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtttt                                                                  >  2:1364412/1‑81 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtttt                                                                  <  1:1364412/81‑1 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAAc                                >  1:5715/1‑115 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAAc                                <  2:5715/115‑1 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                       <  1:1262493/124‑1 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                       <  2:873451/124‑1 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                       >  2:1262493/1‑124 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                       >  1:873451/1‑124 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCggg        >  1:939547/1‑139 (MQ=255)                                                                                                                       tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCggg        >  1:691308/1‑139 (MQ=255)                                                                                                                        atCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc       <  2:73013/139‑1 (MQ=255)                                                                                                                          ccAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                    >  2:622431/1‑44 (MQ=255)                                                                                                                          ccAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                    <  1:622431/44‑1 (MQ=255)                                                                                                                             gACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGttt                                                                   <  2:550013/74‑1 (MQ=255)                                                                                                                             gACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGttt                                                                   >  1:550013/1‑74 (MQ=255)                                                                                                                             gACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGcatcat  >  1:168660/1‑139 (MQ=255)                                                                                                                               cTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTg                                                                 <  1:288821/74‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      TAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCAT  >  NC_000913/824973‑825235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑