Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	829,972	C→T	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ← ybiH ←	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	829,972	0	C	T	100.0%	162.0 / NA	52	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ybiH	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/27);  total (25/27)

ACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGA  >  NC_000913/829839‑830104                                                                                                                                      |                                                                                                                                     aCCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACaa                                                                                                                                 >  2:1419209/1‑139 (MQ=255)  ccGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACaaa                                                                                                                                <  2:1062695/139‑1 (MQ=255)   cGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACa                                                                                                                                  >  1:493130/1‑136 (MQ=255)   cGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACa                                                                                                                                  <  2:493130/136‑1 (MQ=255)     tACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTc                                                                                                                             >  1:266759/1‑139 (MQ=255)       cGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                           <  1:1419209/139‑1 (MQ=255)       cGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCAGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                           >  2:1127711/1‑139 (MQ=255)          atatCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTg                                                                                                                        >  2:1533821/1‑139 (MQ=255)            atCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCg                                                                                                                      <  2:266759/139‑1 (MQ=255)                     gCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCtt                                                                                                             >  2:1198895/1‑139 (MQ=255)                            aGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAAt                                                                                                      <  2:12273/139‑1 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                                    >  1:952312/1‑139 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                                    >  2:308509/1‑139 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                                    >  1:1274099/1‑139 (MQ=255)                               gTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                   <  2:1333083/139‑1 (MQ=255)                               gTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                   <  2:676200/139‑1 (MQ=255)                                tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCaaa                                                                                                        <  2:270509/133‑1 (MQ=255)                                tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCaaa                                                                                                        >  1:270509/1‑133 (MQ=255)                                tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAgg                                                                                                  >  1:107507/1‑139 (MQ=255)                                   ggCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCg                                                                                               <  2:952312/139‑1 (MQ=255)                                   ggCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCg                                                                                               <  1:308509/139‑1 (MQ=255)                                      cGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                   >  1:836644/1‑132 (MQ=255)                                      cGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                   <  2:836644/132‑1 (MQ=255)                                              tGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATGACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                    <  1:1127711/139‑1 (MQ=255)                                                   gcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                               <  1:1533821/139‑1 (MQ=255)                                                   gcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                               <  1:794926/139‑1 (MQ=255)                                                              accaGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                               <  1:1523477/128‑1 (MQ=255)                                                              accaGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                               >  2:1523477/1‑128 (MQ=255)                                                               ccaGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGa                                                                                         <  1:1330244/117‑1 (MQ=255)                                                               ccaGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGa                                                                                         >  2:1330244/1‑117 (MQ=255)                                                                        tCCGGCAACCACGGCGGCAAGTACCATTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCt                                                          >  1:1252473/1‑139 (MQ=255)                                                                        tCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCt                                                          >  1:1361183/1‑139 (MQ=255)                                                                         ccGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCtt                                                         >  2:1263226/1‑139 (MQ=255)                                                                          cGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCttt                                                        >  2:7607/1‑139 (MQ=255)                                                                                         cAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAAcgcg                                         <  1:22964/139‑1 (MQ=255)                                                                                           aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGt                                       <  1:1198895/139‑1 (MQ=255)                                                                                                  cTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAGCTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGCCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCg                                <  1:877953/139‑1 (MQ=255)                                                                                                  cTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCg                                <  2:1361183/139‑1 (MQ=255)                                                                                                       gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACg                           >  1:486877/1‑139 (MQ=255)                                                                                                        ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTttc                                                      <  2:288832/111‑1 (MQ=255)                                                                                                        ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTttc                                                      >  1:288832/1‑111 (MQ=255)                                                                                                        ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGt                          >  1:722306/1‑139 (MQ=255)                                                                                                            tCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGAc                                                                                        >  1:18466/1‑73 (MQ=255)                                                                                                            tCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGAc                                                                                        <  2:18466/73‑1 (MQ=255)                                                                                                            tCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTa                             <  2:585659/132‑1 (MQ=255)                                                                                                            tCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTa                             >  1:585659/1‑132 (MQ=255)                                                                                                             ccGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                           <  2:1324579/37‑1 (MQ=255)                                                                                                             ccGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                           >  1:1324579/1‑37 (MQ=255)                                                                                                                 tCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAAtt                 <  2:1252473/139‑1 (MQ=255)                                                                                                                          gTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGt                                                                 <  1:837511/82‑1 (MQ=255)                                                                                                                          gTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGt                                                                 >  2:837511/1‑82 (MQ=255)                                                                                                                                tcatcaTTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGa  <  1:7607/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     ACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGA  >  NC_000913/829839‑830104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑