Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 854,765 G→A *373* (TAG→TAA ybiR → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913854,7650GA98.1% 167.4 / ‑6.3 53*373* (TAG→TAAybiRputative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (27/25);  minor base T (1/0);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAC  >  NC_000913/854642‑854900
                                                                                                                           |                                                                                                                                       
gATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATaaaa                                                                                                                         <  2:1329380/139‑1 (MQ=255)
 aTTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATaaaa                                                                                                                         <  1:1332697/139‑1 (MQ=255)
                 cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGt                                                                                                         <  1:926882/139‑1 (MQ=255)
                    aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTATGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCg                                                                                                      >  1:927411/1‑139 (MQ=255)
                    aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                                     <  2:751668/124‑1 (MQ=255)
                    aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                                     >  1:751668/1‑124 (MQ=255)
                                       gCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGGTATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                                   <  2:538899/139‑1 (MQ=255)
                                               aTCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGtttt                                                                                    >  1:1072897/1‑130 (MQ=255)
                                               aTCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGtttt                                                                                    <  2:1072897/130‑1 (MQ=255)
                                                   ctATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCa                                                                       <  2:55787/139‑1 (MQ=255)
                                                    tATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAg                                                                      >  2:763263/1‑139 (MQ=255)
                                                      ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGt                                                                                                                                      <  2:956738/73‑1 (MQ=255)
                                                      ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                                                                    >  2:965039/1‑139 (MQ=255)
                                                      ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                                                                    >  1:29765/1‑139 (MQ=255)
                                                      ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACCAAGGt                                                                                                                                      >  1:956738/1‑73 (MQ=255)
                                                           tACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc                                                               >  1:1061446/1‑139 (MQ=255)
                                                                aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                                     <  2:223936/80‑1 (MQ=255)
                                                                aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                                     >  1:223936/1‑80 (MQ=255)
                                                                aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt                                                          >  2:886114/1‑139 (MQ=255)
                                                                aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt                                                          >  2:1084153/1‑139 (MQ=255)
                                                                   cTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATccc                                                       >  1:803505/1‑139 (MQ=255)
                                                                       tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                                   <  2:927411/139‑1 (MQ=255)
                                                                       tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                                   <  2:29765/139‑1 (MQ=255)
                                                                       tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                                   <  1:886114/139‑1 (MQ=255)
                                                                       tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTACTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                                   <  2:1083879/139‑1 (MQ=255)
                                                                         tgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTg                                                                                                                                   >  2:1477229/1‑57 (MQ=255)
                                                                         tgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTg                                                                                                                                   <  1:1477229/57‑1 (MQ=255)
                                                                           ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCGCGGCCGACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGCTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGt                                               >  1:1027315/1‑139 (MQ=255)
                                                                           ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGt                                               >  2:490062/1‑139 (MQ=255)
                                                                           ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGt                                               >  2:448141/1‑139 (MQ=255)
                                                                           ggcggcGTTGGTGGGATATGTTTTGATAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                                     >  2:786426/1‑69 (MQ=255)
                                                                           ggcggcGTTGGTGGGATATGTTTTGATAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                                     <  1:786426/69‑1 (MQ=255)
                                                                               gcgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                           >  1:1142741/1‑139 (MQ=255)
                                                                                cgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTa                                          >  1:103834/1‑139 (MQ=255)
                                                                                cgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTa                                          >  1:248587/1‑139 (MQ=255)
                                                                                 gTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTaa                                         <  1:490062/139‑1 (MQ=255)
                                                                                   tggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                                     >  2:892803/1‑61 (MQ=255)
                                                                                   tggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                                     <  1:892803/61‑1 (MQ=255)
                                                                                      tggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                    <  1:763263/139‑1 (MQ=255)
                                                                                      tggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                    <  2:1117162/139‑1 (MQ=255)
                                                                                                     tagttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAAcc                     <  2:803505/139‑1 (MQ=255)
                                                                                                      agttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTa                                                                                                              >  1:1145206/1‑49 (MQ=255)
                                                                                                      agttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTa                                                                                                              <  2:1145206/49‑1 (MQ=255)
                                                                                                        ttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTgg                  >  1:1472630/1‑139 (MQ=255)
                                                                                                                ccGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATc          >  1:1233362/1‑139 (MQ=255)
                                                                                                                  ggCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                    >  2:633056/1‑111 (MQ=255)
                                                                                                                  ggCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                    <  1:633056/111‑1 (MQ=255)
                                                                                                                  ggCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTg        >  2:242155/1‑139 (MQ=255)
                                                                                                                  ggCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTg        >  2:233661/1‑139 (MQ=255)
                                                                                                                   gCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCttt                                                                                           >  1:865137/1‑55 (MQ=255)
                                                                                                                   gCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCttt                                                                                           <  2:865137/55‑1 (MQ=255)
                                                                                                                        cTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                                   <  1:1287813/90‑1 (MQ=255)
                                                                                                                        cTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc  <  1:448141/139‑1 (MQ=255)
                                                                                                                           |                                                                                                                                       
GATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAC  >  NC_000913/854642‑854900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: