Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969,352	G→A	*329* (TAG→TAA)	lpxK →	lipid A 4'kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969,352	0	G	A	100.0%	115.8 / NA	37	*329* (TAG→TAA)	lpxK	lipid A 4'kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/20);  total (17/20)

AAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAG  >  NC_000913/969225‑969484                                                                                                                                |                                                                                                                                     aaGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCgg                                                                                                                           <  2:1021971/139‑1 (MQ=255)        ggTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGtt                                                                                                                    >  1:731487/1‑139 (MQ=255)                 ccGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATg                                                                                                           <  2:731487/139‑1 (MQ=255)                                    aaTTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGAt                                                                                                                >  2:812324/1‑115 (MQ=255)                                    aaTTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGAt                                                                                                                <  1:812324/115‑1 (MQ=255)                                                 tGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCt                                                                           >  1:1031638/1‑139 (MQ=255)                                                  gCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAc                                                                                   >  2:1159319/1‑130 (MQ=255)                                                  gCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAc                                                                                   <  1:1159319/130‑1 (MQ=255)                                                    cTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCtctc                                                                              <  1:1233595/133‑1 (MQ=255)                                                    cTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCtctc                                                                              >  2:1233595/1‑133 (MQ=255)                                                       tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAc                                                                                   <  2:1352285/125‑1 (MQ=255)                                                       tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAc                                                                                   >  1:1352285/1‑125 (MQ=255)                                                       tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCtctc                                                                              >  2:599304/1‑130 (MQ=255)                                                       tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCtctc                                                                              <  1:599304/130‑1 (MQ=255)                                                           cGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATgcg                                                                 >  2:104872/1‑139 (MQ=255)                                                             cacaGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGcgcg                                                               <  2:432268/139‑1 (MQ=255)                                                                 gCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGACAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGc                                                                                        <  2:872416/110‑1 (MQ=255)                                                                 gCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGACAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGc                                                                                        >  1:872416/1‑110 (MQ=255)                                                                          tgatgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGt                                                                                                       >  1:1524062/1‑86 (MQ=255)                                                                          tgatgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGt                                                                                                       <  2:1524062/86‑1 (MQ=255)                                                                          tgatgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCtt                                                  <  2:301653/139‑1 (MQ=255)                                                                              gaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACctct                                                                               <  2:312889/106‑1 (MQ=255)                                                                              gaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACctct                                                                               >  1:312889/1‑106 (MQ=255)                                                                               aaCCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGc                                             >  1:872363/1‑139 (MQ=255)                                                                                 ccAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGcac                                           >  1:1532395/1‑139 (MQ=255)                                                                                    gCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAg                                        <  1:104872/139‑1 (MQ=255)                                                                                        aaCTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCt                                    <  2:1532395/139‑1 (MQ=255)                                                                                                              gCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGAt                                                                    <  2:1531666/85‑1 (MQ=255)                                                                                                              gCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGAt                                                                    >  1:1531666/1‑85 (MQ=255)                                                                                                               cTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAg             <  2:497481/139‑1 (MQ=255)                                                                                                                tGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGAt                                                                                                            >  1:92046/1‑43 (MQ=255)                                                                                                                tGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGAt                                                                                                            <  2:92046/43‑1 (MQ=255)                                                                                                                tGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGa                                                                                                         >  1:272992/1‑46 (MQ=255)                                                                                                                tGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGa                                                                                                         <  2:272992/46‑1 (MQ=255)                                                                                                                      cTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCg                                                                                      >  2:1365273/1‑59 (MQ=255)                                                                                                                      cTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCg                                                                                      <  1:1365273/59‑1 (MQ=255)                                                                                                                          cAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTggag  <  1:1001292/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                     AAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAG  >  NC_000913/969225‑969484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑