Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,059,014	G→A	V311M (GTG→ATG)	torC →	trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,059,014	0	G	A	100.0%	145.9 / NA	46	V311M (GTG→ATG)	torC	trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

AACGCATCTTTGTTGCCTCGATTCGTGGTGATGTGCAGCAGCAGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGGTGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGA  >  NC_000913/1058880‑1059143                                                                                                                                       |                                                                                                                                  aaCGCATCTTTGTTGCCTCGATTCGTGGTGATGTGCAGCAGCAGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGaa                                                                                                                               <  1:1431274/139‑1 (MQ=255) aaCGCATCTTTGTTGCCTCGATTCGTGGTGATGTGCAGCAGCAGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGaa                                                                                                                               <  2:515071/139‑1 (MQ=255)                           ggtgATGTGCAGCAGCAGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATg                                                                                                     >  1:414727/1‑139 (MQ=255)                                tgtgCAGCAGCAGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGAt                                                                                                >  2:173153/1‑139 (MQ=255)                                 gtgCAGCAGCAGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGAtt                                                                                               <  1:1426127/139‑1 (MQ=255)                                 gtgCAGCAGCAGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGAtt                                                                                               <  2:414727/139‑1 (MQ=255)                                  tgCAGCAGCAGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTc                                                                                              >  1:604603/1‑139 (MQ=255)                                   gcagcagcaGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCg                                                                                             <  2:604603/139‑1 (MQ=255)                                   gcagcagcaGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTAGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGAAGAACGATATCAAACCGATCTGTGCTTATGCGGATTCg                                                                                             <  2:1027144/139‑1 (MQ=255)                                         gcagGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTAc                                                                                       >  1:513250/1‑139 (MQ=255)                                            ggTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAAc                                                                                    >  1:186390/1‑139 (MQ=255)                                              tAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACgg                                                                                  <  2:1174918/139‑1 (MQ=255)                                              tAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACgg                                                                                  <  2:513250/139‑1 (MQ=255)                                                    cGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACgg                                                                                  >  1:273404/1‑133 (MQ=255)                                                    cGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACgg                                                                                  <  2:273404/133‑1 (MQ=255)                                                       tGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATc                                                                                                               <  2:1298344/101‑1 (MQ=255)                                                       tGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATc                                                                                                               >  1:1298344/1‑101 (MQ=255)                                                       tGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTaa                                                                         >  2:264081/1‑139 (MQ=255)                                                                   ccGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACgg                                                                                  >  2:1520251/1‑118 (MQ=255)                                                                   ccGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACgg                                                                                  <  1:1520251/118‑1 (MQ=255)                                                                   ccGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACgg                                                             >  2:1305903/1‑139 (MQ=255)                                                                    cGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGgc                                                            >  1:619496/1‑139 (MQ=255)                                                                    cGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGgc                                                            <  2:186390/139‑1 (MQ=255)                                                                             cacCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGaa                                                   <  2:619496/139‑1 (MQ=255)                                                                                cAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATc                                                >  2:545266/1‑139 (MQ=255)                                                                                        tgTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGaa                                                                                                                               >  2:957496/2‑52 (MQ=255)                                                                                        aGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGaa                                                                                                                               <  1:957496/52‑1 (MQ=255)                                                                                        aGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGaa                                                                                                                               >  2:1348620/1‑52 (MQ=255)                                                                                        aGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGaa                                                                                                                               <  1:1348620/52‑1 (MQ=255)                                                                                        aGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACgg                                                                                  >  1:453744/1‑97 (MQ=255)                                                                                        aGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACgg                                                                                  <  2:453744/97‑1 (MQ=255)                                                                                         gTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGaaa                                                  <  2:169402/128‑1 (MQ=255)                                                                                         gTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGaaa                                                  >  1:169402/1‑128 (MQ=255)                                                                                         gTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACttt                                       <  1:173153/139‑1 (MQ=255)                                                                                          tGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTg                                      >  1:911664/1‑139 (MQ=255)                                                                                          tGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCCCCCACTTTg                                      >  2:316381/1‑139 (MQ=255)                                                                                             aGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACg                                   >  2:361241/1‑139 (MQ=255)                                                                                             aGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACg                                   >  1:684764/1‑139 (MQ=255)                                                                                               cAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCCCCCACTTTGACGCt                                 <  1:316381/139‑1 (MQ=255)                                                                                                         gCCACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGa                       <  1:1163274/139‑1 (MQ=255)                                                                                                           cACTGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATc                     >  2:223585/1‑139 (MQ=255)                                                                                                              tGCGTGGATGAAGAAAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGc                  >  2:1331406/1‑139 (MQ=255)                                                                                                                         agaaAGGCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACgg       <  2:684764/139‑1 (MQ=255)                                                                                                                              ggCGACATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGa  <  2:56167/139‑1 (MQ=255)                                                                                                                                  aCATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCa                                                                >  1:1501488/1‑73 (MQ=255)                                                                                                                                  aCATGATGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCa                                                                <  2:1501488/73‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  AACGCATCTTTGTTGCCTCGATTCGTGGTGATGTGCAGCAGCAGGTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAGGCCACTGCGTGGATGAAGAAAGGCGACATGGTGAACGATATCAAACCGATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGA  >  NC_000913/1058880‑1059143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑