| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,092,286 | A→G | Y2H (TAT→CAT) | pgaA ← | biofilm adhesin polysaccharide PGA secretin, OM porin, poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,092,286 | 0 | A | G | 100.0% | 250.1 / NA | 70 | Y2H (TAT→CAT) | pgaA | biofilm adhesin polysaccharide PGA secretin, OM porin, poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/39); total (31/39) | |||||||||||
CTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGA > NC_000913/1092157‑1092420 | cTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa < 2:1002191/139‑1 (MQ=255) ttCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTAt > 1:557026/1‑139 (MQ=255) ttCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCT‑AAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTAtt < 2:1448619/139‑1 (MQ=255) aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTAGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGt < 2:1339285/139‑1 (MQ=255) aTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTa < 2:980351/139‑1 (MQ=255) tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGTTACTTGAATGCATc < 2:346896/121‑1 (MQ=255) tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGTTACTTGAATGCATc > 1:346896/1‑121 (MQ=255) tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc > 2:448358/1‑121 (MQ=255) tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc < 1:448358/121‑1 (MQ=255) tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAt > 1:670156/1‑139 (MQ=255) catAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCa < 2:945469/139‑1 (MQ=255) gTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCa > 2:1182278/1‑107 (MQ=255) ttattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct > 2:215826/1‑139 (MQ=255) ttattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct < 2:557026/139‑1 (MQ=255) ttattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct < 1:778050/139‑1 (MQ=255) tattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctc > 2:34920/1‑139 (MQ=255) attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct < 2:1313282/139‑1 (MQ=255) attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct > 1:1053567/1‑139 (MQ=255) aaCAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTTCTACTTCAATGCATCCTGTATTACTCCATGTATTGCCAAAATctctctct < 2:180589/138‑1 (MQ=255) aaCAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATctctctct > 1:180589/1‑138 (MQ=255) cAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa > 2:1487781/1‑106 (MQ=255) cAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa < 1:1487781/106‑1 (MQ=255) aGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt < 2:1321736/139‑1 (MQ=255) aGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt < 2:1053567/139‑1 (MQ=255) cACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtct < 2:855189/127‑1 (MQ=255) cACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtct > 1:855189/1‑127 (MQ=255) aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTa > 1:1142838/1‑139 (MQ=255) ctctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTaa < 2:354224/139‑1 (MQ=255) tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc > 2:1277800/1‑95 (MQ=255) tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc < 1:1277800/95‑1 (MQ=255) tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCaaa < 2:1251884/120‑1 (MQ=255) tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCaaa > 1:1251884/1‑120 (MQ=255) tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctc > 1:110649/1‑125 (MQ=255) tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctc < 2:110649/125‑1 (MQ=255) tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAt > 2:485679/1‑139 (MQ=255) tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAt > 1:170954/1‑139 (MQ=255) ttCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc < 2:527521/93‑1 (MQ=255) ttCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc > 1:527521/1‑93 (MQ=255) cgccgcGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct > 1:149123/1‑124 (MQ=255) cgccgcGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct < 2:149123/124‑1 (MQ=255) ccgcGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAgg < 2:867878/139‑1 (MQ=255) cgcgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCAATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCATGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGt > 1:11981/1‑139 (MQ=255) cgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt > 1:1482566/1‑125 (MQ=255) cgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt < 2:1482566/125‑1 (MQ=255) cgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTaa > 2:78387/1‑139 (MQ=255) gggACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTa > 1:245447/1‑102 (MQ=255) gggACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTa < 2:245447/102‑1 (MQ=255) gggACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct > 1:656892/1‑118 (MQ=255) gggACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct < 2:656892/118‑1 (MQ=255) tCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc > 2:334508/1‑80 (MQ=255) tCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc < 1:334508/80‑1 (MQ=255) gCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct < 1:563919/97‑1 (MQ=255) gCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct > 2:563919/1‑97 (MQ=255) ggCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGc < 1:78387/139‑1 (MQ=255) tAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCa > 2:1521091/1‑82 (MQ=255) tAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCa < 1:1521091/82‑1 (MQ=255) agTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAAt < 1:752443/139‑1 (MQ=255) agTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAAt < 2:1142838/139‑1 (MQ=255) cAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAgg < 1:886913/99‑1 (MQ=255) cAAAGCCCATTTGGTTTTCGGGCACCTTTGTCTGCTACTTGAATGTATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAgg > 2:886913/1‑99 (MQ=255) aGCACATTTGGTTTTCAGTGACCTTTATCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAACATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAAGCCGttatt < 2:11981/139‑1 (MQ=255) gCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct > 1:797006/1‑78 (MQ=255) gCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct < 2:797006/78‑1 (MQ=255) tttGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCaaa < 2:437238/65‑1 (MQ=255) tttGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCaaa > 1:437238/1‑65 (MQ=255) ttGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGTACCTCATAATCCGTTATTAAAcgcg < 2:577477/139‑1 (MQ=255) ttGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAAcgcg < 1:215826/139‑1 (MQ=255) tACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct < 1:1045647/47‑1 (MQ=255) tACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct > 2:1045647/1‑47 (MQ=255) gAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATgaga < 1:34920/139‑1 (MQ=255) | CTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGA > NC_000913/1092157‑1092420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |