| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,146,011 | C→T | *195* (TAG→TAA) | yceF ← | m(7)GTP pyrophosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,146,011 | 0 | C | T | 98.4% | 199.3 / NA | 62 | *195* (TAG→TAA) | yceF | m(7)GTP pyrophosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (33/28); minor base G (0/1); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTC > NC_000913/1145884‑1146140 | gCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAg > 2:738924/1‑139 (MQ=255) aCGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGt < 2:1389200/139‑1 (MQ=255) tACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTttcccttcc < 1:738924/139‑1 (MQ=255) gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACg > 1:136873/1‑139 (MQ=255) gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACg > 2:1076309/1‑139 (MQ=255) atatAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa < 1:125931/139‑1 (MQ=255) atatAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa > 2:125931/1‑139 (MQ=255) atatAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa > 2:659175/1‑139 (MQ=255) ataAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa < 2:1498821/137‑1 (MQ=255) ataAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa > 1:1498821/1‑137 (MQ=255) ataAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCAAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAAc > 1:1482552/1‑139 (MQ=255) gATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGTCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGaca > 1:1173176/1‑139 (MQ=255) aTAATGGGCGGTCGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGacac > 2:554027/1‑139 (MQ=255) tGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAgcg > 1:99093/1‑139 (MQ=255) ggCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa > 2:582595/1‑122 (MQ=255) ggCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa < 1:582595/122‑1 (MQ=255) ggCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGcgcg > 1:1096167/1‑139 (MQ=255) ggCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGcgcg > 2:1158001/1‑139 (MQ=255) cGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGAt < 2:99093/139‑1 (MQ=255) ggCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCg > 1:1377905/1‑139 (MQ=255) aGGCGTTTACGCCGCATCAGGTCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑Aaaccaa < 2:1173176/139‑1 (MQ=255) cGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAaccaacca < 1:148125/139‑1 (MQ=255) cGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAaccaacca < 1:659175/139‑1 (MQ=255) gTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAg < 1:1076309/139‑1 (MQ=255) cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAg > 2:1390954/1‑71 (MQ=255) cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAg < 1:1390954/71‑1 (MQ=255) cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAg > 2:1348121/1‑139 (MQ=255) gcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAgg > 1:1153178/1‑139 (MQ=255) aTCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTttcccttccc < 1:1435568/85‑1 (MQ=255) aTCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTttcccttccc > 2:1435568/1‑85 (MQ=255) gccgccAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTttcccttc < 1:94400/78‑1 (MQ=255) gccgccAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTttcccttc > 2:94400/1‑78 (MQ=255) ccAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa < 1:172946/82‑1 (MQ=255) ccAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa > 2:172946/1‑82 (MQ=255) cAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGc > 2:1196510/1‑59 (MQ=255) cAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGc < 1:1196510/59‑1 (MQ=255) gCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAAGTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTa < 2:1153178/139‑1 (MQ=255) aCCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTaaa < 2:136873/139‑1 (MQ=255) ccGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAAAACCAACCAGCGTGTTAGGATCACGCCCCTCTaa > 2:813582/1‑139 (MQ=255) ccGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACg < 1:1292648/128‑1 (MQ=255) ccGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACg > 2:1292648/1‑128 (MQ=255) gATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGc < 1:1348121/139‑1 (MQ=255) gATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGc < 1:1132037/139‑1 (MQ=255) aTTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAaccaacca < 1:458477/109‑1 (MQ=255) aTTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAaccaacca > 2:458477/1‑109 (MQ=255) aTGCGACGTAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTCTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACAAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGc > 2:1454726/1‑139 (MQ=255) tAACCACATCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTAAAACAGCGTAATGcc < 1:1158001/139‑1 (MQ=255) tCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa < 2:555779/49‑1 (MQ=255) tCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa > 1:555779/1‑49 (MQ=255) tCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACg < 1:1325681/101‑1 (MQ=255) tCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACg > 2:1325681/1‑101 (MQ=255) tCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCaa < 2:1155174/118‑1 (MQ=255) tCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCaa > 1:1155174/1‑118 (MQ=255) tCCGGCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCtt < 1:81058/139‑1 (MQ=255) ggCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCg < 2:176005/70‑1 (MQ=255) ggCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCg > 1:176005/1‑70 (MQ=255) ggCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGt < 2:521336/72‑1 (MQ=255) ggCGCAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGt > 1:521336/1‑72 (MQ=255) gcgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCAct > 1:1150332/1‑139 (MQ=255) cgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACg > 2:530328/1‑42 (MQ=255) cgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACg < 1:530328/42‑1 (MQ=255) cgcAAAAAATTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCActc > 2:96328/1‑139 (MQ=255) | GCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGT‑‑AAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTC > NC_000913/1145884‑1146140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |