Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,149,712	G→A	*318* (TAG→TAA)	fabH →	3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,149,712	0	G	A	100.0%	150.5 / NA	47	*318* (TAG→TAA)	fabH	3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/25);  total (22/25)

AATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGG  >  NC_000913/1149578‑1149842                                                                                                                                       |                                                                                                                                    aaTACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa                                                                                                                                 <  2:944577/139‑1 (MQ=255) aaTACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa                                                                                                                                 <  1:4808/139‑1 (MQ=255)      ctctGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGAt                                                                                                                            <  1:713784/139‑1 (MQ=255)              ccTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACAt                                                                                                                    >  1:388020/1‑139 (MQ=255)                tctGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGa                                                                                                                  >  2:1106699/1‑139 (MQ=255)                tctGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCAACTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGa                                                                                                                  <  2:41807/139‑1 (MQ=255)                         tGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTg                                                                                                         >  2:1193399/1‑139 (MQ=255)                            gcgcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCAt                                                                                                      <  2:456590/139‑1 (MQ=255)                                tGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                  <  2:1115188/139‑1 (MQ=255)                                         cTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTgg                                                                                        <  1:1106699/139‑1 (MQ=255)                                            tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                  <  2:1290272/127‑1 (MQ=255)                                            tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                  >  1:1290272/1‑127 (MQ=255)                                            tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACa                                                                                     >  2:1117505/1‑139 (MQ=255)                                                   cGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTtct                                                                              >  1:1155276/1‑139 (MQ=255)                                                    gggCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa                                                                                                                                 <  1:66568/88‑1 (MQ=255)                                                    gggCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa                                                                                                                                 >  2:66568/1‑88 (MQ=255)                                                      gcgcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa                                                                                                                                 >  2:850447/1‑86 (MQ=255)                                                      gcgcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa                                                                                                                                 <  1:850447/86‑1 (MQ=255)                                                        gcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAAc                                                                         <  2:1351467/139‑1 (MQ=255)                                                        gcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAAc                                                                         <  1:1193399/139‑1 (MQ=255)                                                           ttAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCa                                                                                                              >  1:743344/1‑100 (MQ=255)                                                           ttAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCa                                                                                                              <  2:743344/100‑1 (MQ=255)                                                                     gCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATgctg                                                            <  1:1117505/139‑1 (MQ=255)                                                                          tGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTTCCCTGGACAGGGTTCTCAAACCGTTGGAATgctggctg                                                        >  2:676892/1‑139 (MQ=255)                                                                                 gCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcg                                                >  1:167238/1‑139 (MQ=255)                                                                                   ttGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcggc                                              <  1:339138/139‑1 (MQ=255)                                                                                    tGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGTTGGCTGATATGgcggcg                                             <  2:1046381/139‑1 (MQ=255)                                                                                    tGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGgcggcg                                             <  2:167238/139‑1 (MQ=255)                                                                                            ttGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCAt                                                                                                      >  1:561931/1‑75 (MQ=255)                                                                                            ttGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCAt                                                                                                      <  2:561931/75‑1 (MQ=255)                                                                                             tGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAAt                                                                                                            <  2:326451/68‑1 (MQ=255)                                                                                             tGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAAt                                                                                                            >  1:326451/1‑68 (MQ=255)                                                                                                    ggATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCg                             <  2:1155276/139‑1 (MQ=255)                                                                                                               gggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGttt                  >  2:367004/1‑139 (MQ=255)                                                                                                               gggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGttt                  >  1:1178517/1‑139 (MQ=255)                                                                                                               gggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGttt                  >  1:1265705/1‑139 (MQ=255)                                                                                                                    ccGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCa                                                                                                              >  2:1492246/1‑43 (MQ=255)                                                                                                                    ccGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCa                                                                                                              <  1:1492246/43‑1 (MQ=255)                                                                                                                    ccGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                  >  2:461251/1‑55 (MQ=255)                                                                                                                    ccGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                  <  1:461251/55‑1 (MQ=255)                                                                                                                     cgcgCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                  <  1:814819/54‑1 (MQ=255)                                                                                                                     cgcgCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                  >  2:814819/1‑54 (MQ=255)                                                                                                                     cgcgCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACACGGTTCTCa                                                                            <  2:105462/75‑1 (MQ=255)                                                                                                                     cgcgCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACACGGTTCTCa                                                                            >  1:105462/1‑75 (MQ=255)                                                                                                                               cGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTgcgg  >  2:379558/1‑139 (MQ=255)                                                                                                                                 tttCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTg                                >  2:1474104/1‑107 (MQ=255)                                                                                                                                 tttCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTg                                <  1:1474104/107‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    AATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTG‑TTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGG  >  NC_000913/1149578‑1149842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑