Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 104,352 A→G E124G (GAG→GGG)  ftsA → ATP‑binding cell division FtsK recruitment protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913104,3520AG100.0% 215.7 / NA 62E124G (GAG→GGG) ftsAATP‑binding cell division FtsK recruitment protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/30);  total (32/30)

TATATCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGG  >  NC_000913/104217‑104483
                                                                                                                                       |                                                                                                                                   
tataTCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCa                                                                                                                                  >  2:351120/1‑139 (MQ=255)
tataTCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTCCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCa                                                                                                                                  <  2:1101090/139‑1 (MQ=255)
   atCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCg                                                                                                                               >  1:359773/1‑139 (MQ=255)
     cTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                   >  1:1534847/1‑133 (MQ=255)
       ggCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                           >  2:1455912/1‑139 (MQ=255)
       ggCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                           <  1:499716/139‑1 (MQ=255)
       ggCGCTTTCTGGTAAGCACACCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                           <  1:63044/139‑1 (MQ=255)
              tCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtg                                                                                                                    >  2:1509236/1‑139 (MQ=255)
                  gTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                           <  2:757880/128‑1 (MQ=255)
                  gTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                           >  1:757880/1‑128 (MQ=255)
                     aGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATccc                                                                                                             <  1:1455912/139‑1 (MQ=255)
                      gCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCg                                                                                                            >  1:1213628/1‑139 (MQ=255)
                         caTCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCaa                                                                                                         <  1:351120/139‑1 (MQ=255)
                          aTCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAag                                                                                                        >  2:650229/1‑139 (MQ=255)
                            cAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                           >  2:443132/1‑118 (MQ=255)
                            cAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                           <  1:443132/118‑1 (MQ=255)
                            cAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAagag                                                                                                      >  1:832275/1‑139 (MQ=255)
                                 gCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGc                                                                                                 >  1:497835/1‑139 (MQ=255)
                                       aTGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGa                                                                                           <  1:1509236/139‑1 (MQ=255)
                                         gagacaggTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCAGACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGCGTGCTGCATGTGATCCCGCAATAGTATGCGATTGACt                                                                                         <  2:1027891/133‑1 (MQ=255)
                                               ggTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAgg                                                                                   >  1:1507684/1‑139 (MQ=255)
                                                gTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgct                                                                                                                         >  1:212932/1‑100 (MQ=255)
                                                gTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgct                                                                                                                         <  2:212932/100‑1 (MQ=255)
                                                  aTGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAg                                                                                <  2:497835/139‑1 (MQ=255)
                                                      tGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGAt                                                                            >  2:1672/1‑139 (MQ=255)
                                                          tATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                   <  1:698740/80‑1 (MQ=255)
                                                          tATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                   >  2:698740/1‑80 (MQ=255)
                                                          tATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                           <  2:831158/88‑1 (MQ=255)
                                                          tATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                           >  1:831158/1‑88 (MQ=255)
                                                           aTTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAGCGTCGGCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATACCGCAAGAGTATGCGATTGACTATCAGGAAgggctgtctc                                                                       >  1:1027891/1‑132 (MQ=255)
                                                                tGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgt                                                                                                                              >  1:1051598/1‑79 (MQ=255)
                                                                tGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgt                                                                                                                              <  2:1051598/79‑1 (MQ=255)
                                                                  aagaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgc                                                                                                                          >  1:131336/1‑81 (MQ=255)
                                                                  aagaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgc                                                                                                                          <  2:131336/81‑1 (MQ=255)
                                                                  aagaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGt                                                                                                     <  2:984874/102‑1 (MQ=255)
                                                                  aagaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGt                                                                                                     >  1:984874/1‑102 (MQ=255)
                                                                                         gtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGc                                         <  2:474600/139‑1 (MQ=255)
                                                                                                 cgtcgtCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACttt                                                        >  1:1036381/1‑116 (MQ=255)
                                                                                                 cgtcgtCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACttt                                                        <  2:1036381/116‑1 (MQ=255)
                                                                                                  gtcgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGAt                                           >  2:768742/1‑128 (MQ=255)
                                                                                                  gtcgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGAt                                           <  1:768742/128‑1 (MQ=255)
                                                                                                    cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTg                              >  2:1142159/1‑139 (MQ=255)
                                                                                                      tcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtgt                                                                                                                   >  2:225856/1‑52 (MQ=255)
                                                                                                      tcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtgt                                                                                                                   <  1:225856/52‑1 (MQ=255)
                                                                                                       ccATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCAc                           <  1:650229/139‑1 (MQ=255)
                                                                                                       ccATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCAc                           <  2:1213628/139‑1 (MQ=255)
                                                                                                          tACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTg                        >  2:455691/1‑139 (MQ=255)
                                                                                                            ccGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                 <  1:983479/80‑1 (MQ=255)
                                                                                                            ccGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                 >  2:983479/1‑80 (MQ=255)
                                                                                                                gAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcaca                  <  1:1142159/139‑1 (MQ=255)
                                                                                                                gAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcaca                  <  1:455691/139‑1 (MQ=255)
                                                                                                                 aaaTCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCg                                                                                                            >  1:1077126/1‑48 (MQ=255)
                                                                                                                 aaaTCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCg                                                                                                            <  2:1077126/48‑1 (MQ=255)
                                                                                                                       gTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa                                                               <  2:342166/87‑1 (MQ=255)
                                                                                                                       gTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa                                                               >  1:342166/1‑87 (MQ=255)
                                                                                                                                cgcgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGata     <  1:442084/136‑1 (MQ=255)
                                                                                                                                cgcgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGata     >  2:442084/1‑136 (MQ=255)
                                                                                                                                cgcgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATgg  >  2:344771/1‑139 (MQ=255)
                                                                                                                                  cgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGa                                                                                  <  2:1288298/57‑1 (MQ=255)
                                                                                                                                  cgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGa                                                                                  >  1:1288298/1‑57 (MQ=255)
                                                                                                                                    aTGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa                                                               <  1:272910/74‑1 (MQ=255)
                                                                                                                                    aTGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa                                                               >  2:272910/1‑74 (MQ=255)
                                                                                                                                       |                                                                                                                                   
TATATCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGG  >  NC_000913/104217‑104483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑