Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 111,433 G→A *130* (TAG→TAA mutT → dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913111,4330GA100.0% 97.8 / NA 31*130* (TAG→TAAmutTdGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/18);  total (13/18)

GTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGT  >  NC_000913/111302‑111551
                                                                                                                                   |                                                                                                                      
gTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGAt                                                                                                                 >  1:1025362/1‑139 (MQ=255)
             aaGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgc                                                                                                    <  2:334402/139‑1 (MQ=255)
                   aGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATc                                                                                              >  1:512945/1‑139 (MQ=255)
                    gCCGTGGGGTAAAGTAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATcc                                                                                             <  2:1001112/139‑1 (MQ=255)
                     ccGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCg                                                                                            >  1:1430127/1‑139 (MQ=255)
                      cGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGGTTTCGCCGCATCCGa                                                                                           <  2:1025362/139‑1 (MQ=255)
                            gTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCg                                                                                     <  2:1446959/139‑1 (MQ=255)
                                 gaagGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCacac                                                                                <  2:894608/139‑1 (MQ=255)
                                     ggCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCg                                                                                     >  2:1102175/1‑130 (MQ=255)
                                     ggCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCg                                                                                     <  1:1102175/130‑1 (MQ=255)
                                                     gATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTgg                                                            <  2:512945/139‑1 (MQ=255)
                                                       tGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCTAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATc                                                                                              <  2:1086810/103‑1 (MQ=255)
                                                       tGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATc                                                                                              >  1:1086810/1‑103 (MQ=255)
                                                        gTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgc                                                                                                    >  2:1467211/1‑96 (MQ=255)
                                                        gTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgc                                                                                                    <  1:1467211/96‑1 (MQ=255)
                                                                 cggtcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCa                                                <  2:1326090/139‑1 (MQ=255)
                                                                            ccGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                     <  2:1430127/139‑1 (MQ=255)
                                                                              gatgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCtt                                                    <  1:58972/122‑1 (MQ=255)
                                                                              gatgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCtt                                                    >  2:58972/1‑122 (MQ=255)
                                                                                                  tGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAAtt               >  2:655069/1‑139 (MQ=255)
                                                                                                       cGGTAATTCCGAAGCTTAAACGTCTGTAAGTCAGAt                                                                                                                 <  1:161874/36‑1 (MQ=255)
                                                                                                       cGGTAATTCCGAAGCTTAAACGTCTGTAAGTCAGAt                                                                                                                 >  2:161874/1‑36 (MQ=255)
                                                                                                        ggTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCg                                                                                     <  2:1285338/63‑1 (MQ=255)
                                                                                                        ggTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCg                                                                                     >  1:1285338/1‑63 (MQ=255)
                                                                                                        ggTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACg                                                                               <  1:796372/69‑1 (MQ=255)
                                                                                                        ggTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACg                                                                               >  2:796372/1‑69 (MQ=255)
                                                                                                         gTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGttttt        >  2:274816/1‑139 (MQ=255)
                                                                                                               gCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGt  <  2:482726/139‑1 (MQ=255)
                                                                                                               gCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGt  <  1:655069/139‑1 (MQ=255)
                                                                                                                   aGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGt                                                       >  1:419968/1‑82 (MQ=255)
                                                                                                                   aGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGt                                                       <  2:419968/82‑1 (MQ=255)
                                                                                                                                   |                                                                                                                      
GTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGT  >  NC_000913/111302‑111551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: