Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,228,724	A→G	T216A (ACT→GCT)	ycgM →	putative isomerase/hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,228,724	0	A	G	100.0%	153.0 / NA	43	T216A (ACT→GCT)	ycgM	putative isomerase/hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/24);  total (19/24)

TGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCA  >  NC_000913/1228593‑1228852                                                                                                                                    |                                                                                                                                 tGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGt                                                                                                                           <  1:1122189/139‑1 (MQ=255)  gATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGtt                                                                                                                          <  2:669432/139‑1 (MQ=255)       cTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACGGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTa                                                                                                                     <  1:1415229/139‑1 (MQ=255)             tGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACtt                                                                                                               >  2:431219/1‑139 (MQ=255)                 cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTg                                                                                                           >  1:1176007/1‑139 (MQ=255)                             ccTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAgc                                                                                               <  2:1244386/139‑1 (MQ=255)                               tCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAgcgg                                                                                             >  2:1165181/1‑139 (MQ=255)                                        gTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAAc                                                                                    >  2:1373657/1‑139 (MQ=255)                                                        aGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCAt                                                                              >  1:1075196/1‑129 (MQ=255)                                                        aGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCAt                                                                              <  2:1075196/129‑1 (MQ=255)                                                           cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcg                                                                                                                              <  2:627788/78‑1 (MQ=255)                                                           cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcg                                                                                                                              >  1:627788/1‑78 (MQ=255)                                                           cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                 <  2:1300291/139‑1 (MQ=255)                                                           cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                 >  1:1300291/1‑139 (MQ=255)                                                           cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                 <  2:453551/139‑1 (MQ=255)                                                             cGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTg                                                               >  1:73198/1‑139 (MQ=255)                                                               ccTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGtt                                                                                                                          >  1:1504631/1‑78 (MQ=255)                                                               ccTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGtt                                                                                                                          <  2:1504631/78‑1 (MQ=255)                                                                   aTGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                 <  1:1518071/131‑1 (MQ=255)                                                                   aTGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                 >  2:1518071/1‑131 (MQ=255)                                                                              ccGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGtttt                                              <  1:1049891/139‑1 (MQ=255)                                                                                 ttGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAgcg                                                                                              >  1:65076/1‑88 (MQ=255)                                                                                 ttGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAgcg                                                                                              <  2:65076/88‑1 (MQ=255)                                                                                         cGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGc                                   <  1:431219/139‑1 (MQ=255)                                                                                           gTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTg                                                                                                           <  2:322837/65‑1 (MQ=255)                                                                                           gTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTg                                                                                                           >  1:322837/1‑65 (MQ=255)                                                                                            tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTgccg                                                                                                        <  2:954549/67‑1 (MQ=255)                                                                                            tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTgccg                                                                                                        >  1:954549/1‑67 (MQ=255)                                                                                            tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCtgtg                                                                       <  2:1514070/100‑1 (MQ=255)                                                                                            tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCtgtg                                                                       >  1:1514070/1‑100 (MQ=255)                                                                                            tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCgg                                 <  2:1293436/138‑1 (MQ=255)                                                                                            tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCgg                                 >  1:1293436/1‑138 (MQ=255)                                                                                                        aGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAg                    <  1:1373657/139‑1 (MQ=255)                                                                                                          tCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCg                  <  2:1176007/139‑1 (MQ=255)                                                                                                             cTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCgg                                 <  2:1055827/121‑1 (MQ=255)                                                                                                             cTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCgg                                 >  1:1055827/1‑121 (MQ=255)                                                                                                                tCGATGGGCATTCTTTGACAGCTCGCGTTTTATAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgat                       <  1:952787/128‑1 (MQ=255)                                                                                                                tCGATGGGCATTCTTTGACAGCTCGCGTTTTATAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgat                       >  2:952787/1‑128 (MQ=255)                                                                                                                   aTGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCa                                                                                        >  2:1250075/1‑60 (MQ=255)                                                                                                                   aTGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCa                                                                                        <  1:1250075/60‑1 (MQ=255)                                                                                                                          ttCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCa  <  2:73198/139‑1 (MQ=255)                                                                                                                            cTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCtg                                                                         >  2:104449/1‑66 (MQ=255)                                                                                                                            cTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCtg                                                                         <  1:104449/66‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 TGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCA  >  NC_000913/1228593‑1228852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑