Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,309,016	C→T	*418* (TAG→TAA)	kch ←	voltage‑gated potassium channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,309,016	0	C	T	100.0%	187.1 / NA	58	*418* (TAG→TAA)	kch	voltage‑gated potassium channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/30);  total (28/30)

CATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTC  >  NC_000913/1308898‑1309132                                                                                                                       |                                                                                                                     cATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                           >  2:1270132/1‑130 (MQ=255) cATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                           <  1:1270132/130‑1 (MQ=255)    cAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                           <  2:115764/127‑1 (MQ=255)    cAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                           >  1:115764/1‑127 (MQ=255)           tGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                        >  2:222298/1‑139 (MQ=255)                         acaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTc                                                                                     <  1:481094/128‑1 (MQ=255)                         acaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTc                                                                                     >  2:481094/1‑128 (MQ=255)                                   tCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTc                                                                          >  2:1498816/1‑129 (MQ=255)                                   tCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTc                                                                          <  1:1498816/129‑1 (MQ=255)                                       tATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                           >  1:781672/1‑92 (MQ=255)                                       tATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                           <  2:781672/92‑1 (MQ=255)                                              tGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATAcc                                                     <  1:1355613/139‑1 (MQ=255)                                                   tCTACGATATCTAAATGAACTGTGCAATATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTTCGCCGATTCTTTACTGTGAGCTTTCGTTTCTAGTTCATCATTATCCATGAAAATACCATGAc                                                <  1:1190348/139‑1 (MQ=255)                                                     tACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                              <  2:1117262/139‑1 (MQ=255)                                                               aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGtt                                                                           >  2:1072425/1‑100 (MQ=255)                                                               aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGtt                                                                           <  1:1072425/100‑1 (MQ=255)                                                               aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                    >  1:18014/1‑139 (MQ=255)                                                               aaaTGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                    >  2:936925/1‑139 (MQ=255)                                                                    tACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                       <  2:1007073/83‑1 (MQ=255)                                                                    tACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                       >  1:1007073/1‑83 (MQ=255)                                                                      cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGt                                                                                   <  2:980066/85‑1 (MQ=255)                                                                      cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGt                                                                                   >  1:980066/1‑85 (MQ=255)                                                                      cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                <  1:738064/104‑1 (MQ=255)                                                                      cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                >  2:738064/1‑104 (MQ=255)                                                                      cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATAcc                                                     <  1:1118853/115‑1 (MQ=255)                                                                      cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATAcc                                                     >  2:1118853/1‑115 (MQ=255)                                                                      cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGa                             >  1:890868/1‑139 (MQ=255)                                                                        gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                           <  1:1203467/59‑1 (MQ=255)                                                                        gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                           >  2:1203467/1‑59 (MQ=255)                                                                        gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaaa                           <  1:1107650/139‑1 (MQ=255)                                                                        gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaaa                           >  2:1477127/1‑139 (MQ=255)                                                                        gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaaa                           <  1:433128/139‑1 (MQ=255)                                                                         ggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAAc                          >  2:1240835/1‑139 (MQ=255)                                                                             ccATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGc                      <  2:18014/139‑1 (MQ=255)                                                                              cATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAacaaca                                 >  1:329244/1‑127 (MQ=255)                                                                              cATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAacaaca                                 <  2:329244/127‑1 (MQ=255)                                                                              cATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCa                     >  2:1410314/1‑139 (MQ=255)                                                                                 tGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCatat                  <  1:499916/139‑1 (MQ=255)                                                                                    ggTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATGCCATGACCTGAGTTCAACAACATTGAAACGAGTATATCat               <  1:936925/139‑1 (MQ=255)                                                                                        cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                      <  1:207690/112‑1 (MQ=255)                                                                                        cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                      >  2:207690/1‑112 (MQ=255)                                                                                        cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCattatt           >  2:1392160/1‑139 (MQ=255)                                                                                         ccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCAttatta          <  1:1240835/139‑1 (MQ=255)                                                                                          cAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCatta             >  1:796542/1‑135 (MQ=255)                                                                                          cAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCatta             <  2:796542/135‑1 (MQ=255)                                                                                          cAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTaa         >  2:255848/1‑139 (MQ=255)                                                                                           aaTAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGt                                                                            >  1:558181/1‑71 (MQ=255)                                                                                           aaTAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGt                                                                            <  2:558181/71‑1 (MQ=255)                                                                                           aaTAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAAt        <  1:1221034/139‑1 (MQ=255)                                                                                                 tttACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTc  <  1:255848/139‑1 (MQ=255)                                                                                                  ttACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                              <  1:739541/94‑1 (MQ=255)                                                                                                  ttACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                              >  2:739541/1‑94 (MQ=255)                                                                                                  ttACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCattatt           >  1:611086/1‑129 (MQ=255)                                                                                                  ttACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCattatt           <  2:611086/129‑1 (MQ=255)                                                                                                    aCTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                >  1:1340745/1‑74 (MQ=255)                                                                                                    aCTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCg                                                                <  2:1340745/74‑1 (MQ=255)                                                                                                          ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaaa                           <  2:288244/105‑1 (MQ=255)                                                                                                          ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGaaa                           >  1:288244/1‑105 (MQ=255)                                                                                                                       |                                                                                                                     CATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTC  >  NC_000913/1308898‑1309132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑