Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,322,601	C→T	A116T (GCC→ACC)	trpE ←	component I of anthranilate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,322,601	0	C	T	100.0%	213.3 / NA	66	A116T (GCC→ACC)	trpE	component I of anthranilate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/34);  total (32/34)

AAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAG  >  NC_000913/1322465‑1322730                                                                                                                                         |                                                                                                                                  aaaTCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGgtgt                                                                                                                                 <  1:360766/139‑1 (MQ=255)         aaaTCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATcc                                                                                                                         >  2:300103/1‑139 (MQ=255)            tCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc                                                                                                                      <  2:317185/139‑1 (MQ=255)              ccGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    >  2:1491647/1‑139 (MQ=255)              ccGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    >  2:1487851/1‑139 (MQ=255)                gCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGGTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTg                                                                                                                  >  2:224396/1‑139 (MQ=255)                 ccACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTgg                                                                                                                 >  1:765072/1‑139 (MQ=255)                    caAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACt                                                                                                              <  1:627186/139‑1 (MQ=255)                    caAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACt                                                                                                              <  1:902037/139‑1 (MQ=255)                              agagAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGGTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg                                                                                                    <  1:224396/139‑1 (MQ=255)                                  aaCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAgc                                                                                                >  2:398362/1‑139 (MQ=255)                                   aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    <  2:1312664/118‑1 (MQ=255)                                   aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    >  1:1312664/1‑118 (MQ=255)                                   aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg                                                                                                    <  1:242171/134‑1 (MQ=255)                                   aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg                                                                                                    >  2:242171/1‑134 (MQ=255)                                   aCAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAgcg                                                                                               <  2:1279283/139‑1 (MQ=255)                                     aGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCgca                                                                                             <  1:889425/139‑1 (MQ=255)                                       gccgccGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    <  1:467173/114‑1 (MQ=255)                                       gccgccGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    >  2:467173/1‑114 (MQ=255)                                          gccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAg                                                                                                         <  2:526512/122‑1 (MQ=255)                                          gccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAg                                                                                                         >  1:526512/1‑122 (MQ=255)                                          gccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCgcagca                                                                                          <  2:952934/137‑1 (MQ=255)                                          gccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGcaca                                                                                        >  1:734836/1‑139 (MQ=255)                                          gccgAAGAACATGGCTTCTCGTTCTTCCCTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTAAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCgcagca                                                                                          >  1:952934/1‑137 (MQ=255)                                           ccgAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCacac                                                                                       >  2:493273/1‑139 (MQ=255)                                                     tGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTgg                                                                             <  2:231077/139‑1 (MQ=255)                                                     tGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTgg                                                                             <  2:951330/139‑1 (MQ=255)                                                      ggCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc                                                                                                                      <  1:1497537/97‑1 (MQ=255)                                                      ggCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc                                                                                                                      >  2:1497537/1‑97 (MQ=255)                                                      ggCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGt                                                                            >  2:962617/1‑139 (MQ=255)                                                      ggCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGt                                                                            >  2:260254/1‑139 (MQ=255)                                                           ctcGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTg                                                                       >  2:1273443/1‑139 (MQ=255)                                                            tcGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGt                                                                      <  1:1491647/139‑1 (MQ=255)                                                              gTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc                                                                                                                      <  2:1527556/89‑1 (MQ=255)                                                              gTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagc                                                                                                                      >  1:1527556/1‑89 (MQ=255)                                                                        cGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCcaca                                                          >  1:701701/1‑139 (MQ=255)                                                                              aTTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGTGGGGAAGCGCAGCACACgg                                                                                     <  1:1022602/106‑1 (MQ=255)                                                                              aTTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACgg                                                                                     >  2:1022602/1‑106 (MQ=255)                                                                               ttCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCgcag                                                   >  2:117074/1‑139 (MQ=255)                                                                                  aaCAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCgcaggca                                                <  1:300103/139‑1 (MQ=255)                                                                                     aGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAggg                                             >  2:439636/1‑139 (MQ=255)                                                                                       aTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCg                                           <  1:1273443/139‑1 (MQ=255)                                                                                       aTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCg                                           <  2:765072/139‑1 (MQ=255)                                                                                       aTTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCg                                           <  1:493273/139‑1 (MQ=255)                                                                                        ttCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCa                                                                                                                            >  2:105219/1‑57 (MQ=255)                                                                                        ttCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCa                                                                                                                            <  1:105219/57‑1 (MQ=255)                                                                                         tCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGAc                                                                                                               <  1:394948/69‑1 (MQ=255)                                                                                         tCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGAc                                                                                                               >  2:394948/1‑69 (MQ=255)                                                                                         tCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGc                                                                                    <  2:572769/96‑1 (MQ=255)                                                                                         tCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGc                                                                                    >  1:572769/1‑96 (MQ=255)                                                                                          cTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    <  1:1433294/63‑1 (MQ=255)                                                                                          cTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    >  2:1433294/1‑63 (MQ=255)                                                                                          cTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTa                                        <  1:1487851/139‑1 (MQ=255)                                                                                            gCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCt                                                                                                                               >  1:62469/1‑50 (MQ=255)                                                                                            gCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCt                                                                                                                               <  2:62469/50‑1 (MQ=255)                                                                                                 aaaCGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    >  1:871065/1‑56 (MQ=255)                                                                                                 aaaCGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCcagcag                                                                                                                    <  2:871065/56‑1 (MQ=255)                                                                                                         aGCGTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCagg                         <  1:260254/139‑1 (MQ=255)                                                                                                          gCGTCAAAAACCGAAAGGGAGCATAAGC‑GGTGTCTTCATCCAGCAGTGGACTGACAgggggg                                                                                                    >  1:916565/1‑62 (MQ=255)                                                                                                          gCGTCAAAAACCGAAAGGGAGCATAAGC‑GGTGTCTTCATCCAGCAGTGGACTGACAgggggg                                                                                                    <  2:916565/62‑1 (MQ=38)                                                                                                            gTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg                                                                                                    >  2:681934/1‑61 (MQ=255)                                                                                                            gTCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAgggggg                                                                                                    <  1:681934/61‑1 (MQ=255)                                                                                                             tCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCca                                                            <  2:875552/100‑1 (MQ=255)                                                                                                             tCAAAAACCGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCca                                                            >  1:875552/1‑100 (MQ=255)                                                                                                                     cGAAAGGGAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTCTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCg             >  2:263764/1‑139 (MQ=255)                                                                                                                            gAGCATAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCgg      <  2:701701/139‑1 (MQ=255)                                                                                                                                aTAAGCGGGTGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAg  <  1:439636/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  AAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAG  >  NC_000913/1322465‑1322730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑