Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	122,856	G→A	*255* (TAG→TAA)	pdhR →	pyruvate dehydrogenase complex repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	122,856	0	G	A	100.0%	165.6 / NA	51	*255* (TAG→TAA)	pdhR	pyruvate dehydrogenase complex repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/29);  total (22/29)

GAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAGTGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAA  >  NC_000913/122737‑122989                                                                                                                        |                                                                                                                                      gaagCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAAt                                                                                                                    >  1:502729/1‑139 (MQ=255)         cgAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCaga                                                                                                            <  2:1508110/139‑1 (MQ=255)           aaGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaag                                                                                                          <  1:1339675/139‑1 (MQ=255)                        ccATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTAt                                                                                                                 >  1:687027/1‑119 (MQ=255)                        ccATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTAt                                                                                                                 <  2:687027/119‑1 (MQ=255)                        ccATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCa                                                                                             >  1:771930/1‑139 (MQ=255)                           tCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc                                                                                          <  1:641570/139‑1 (MQ=255)                                     aTCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaag                                                                                                          <  2:241363/113‑1 (MQ=255)                                     aTCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaag                                                                                                          >  1:241363/1‑113 (MQ=255)                                         aagaaATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGt                                                                                                                          >  2:635698/1‑93 (MQ=255)                                         aagaaATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGt                                                                                                                          <  1:635698/93‑1 (MQ=255)                                         aagaaATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAAtt                                                                                                                   <  2:198643/100‑1 (MQ=255)                                         aagaaATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAAtt                                                                                                                   >  1:198643/1‑100 (MQ=255)                                                tttGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACt                                                                     <  2:1438619/139‑1 (MQ=255)                                                 ttGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTa                                                                    <  1:399573/139‑1 (MQ=255)                                                 ttGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTa                                                                    <  2:771930/139‑1 (MQ=255)                                                              gTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTg                                                       <  2:502729/139‑1 (MQ=255)                                                                         agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc                                                                                          >  1:570136/1‑93 (MQ=255)                                                                         agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc                                                                                          >  1:119219/1‑93 (MQ=255)                                                                         agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc                                                                                          <  2:119219/93‑1 (MQ=255)                                                                         agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc                                                                                          <  2:570136/93‑1 (MQ=255)                                                                         agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGc                                            >  2:877924/1‑139 (MQ=255)                                                                         agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGc                                            >  1:82204/1‑139 (MQ=255)                                                                           ccgccgTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaag                                                                                                          <  2:1308984/75‑1 (MQ=255)                                                                           ccgccgTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaag                                                                                                          >  1:1308984/1‑75 (MQ=255)                                                                                   aGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCga                                  <  2:79511/139‑1 (MQ=255)                                                                                         ctctGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTc                            <  1:877924/139‑1 (MQ=255)                                                                                           ctGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaaga                                                                                                         <  2:665545/60‑1 (MQ=255)                                                                                           ctGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaaga                                                                                                         >  1:665545/1‑60 (MQ=255)                                                                                              cgtcgtCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATgg                       >  1:197528/1‑139 (MQ=255)                                                                                                 cgtcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACt                                                                     <  1:1167128/90‑1 (MQ=255)                                                                                                 cgtcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACt                                                                     >  2:1167128/1‑90 (MQ=255)                                                                                                   tcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGAtgtt                                                                                                     <  1:58877/56‑1 (MQ=255)                                                                                                   tcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGAtgtt                                                                                                     >  2:58877/1‑56 (MQ=255)                                                                                                   tcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCa                           <  1:611951/130‑1 (MQ=255)                                                                                                   tcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCa                           >  2:611951/1‑130 (MQ=255)                                                                                                        aGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGtt               >  2:668882/1‑137 (MQ=255)                                                                                                        aGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGtt               <  1:668882/137‑1 (MQ=255)                                                                                                             cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaaga                                                                                                         >  1:922796/1‑42 (MQ=255)                                                                                                             cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaag                                                                                                          <  2:1343711/41‑1 (MQ=255)                                                                                                             cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCagaag                                                                                                          >  1:1343711/1‑41 (MQ=255)                                                                                                             cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGagag                               >  2:1140785/1‑116 (MQ=255)                                                                                                             cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGagag                               <  1:1140785/116‑1 (MQ=255)                                                                                                             cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGAc                    <  1:993449/127‑1 (MQ=255)                                                                                                             cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGAc                    >  2:993449/1‑127 (MQ=255)                                                                                                             cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGaaa        >  2:620201/1‑139 (MQ=255)                                                                                                             cGAAAGAATTAATGATTTTTATGGTAAAAATTATCCagaaga                                                                                                         <  2:922796/42‑1 (MQ=255)                                                                                                                   aaTTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa  <  2:708164/139‑1 (MQ=255)                                                                                                                   aaTTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa  <  2:1236428/139‑1 (MQ=255)                                                                                                                   aaTTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa  <  2:1371805/139‑1 (MQ=255)                                                                                                                   aaTTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa  <  2:82204/139‑1 (MQ=255)                                                                                                                        |                                                                                                                                      GAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAGTGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAA  >  NC_000913/122737‑122989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑