Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	123,475	C→T	F153F (TTC→TTT)	aceE →	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	123,475	0	C	T	100.0%	193.4 / NA	60	F153F (TTC→TTT)	aceE	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/32);  total (28/32)

TCCTTCCAGTCTTCCGCAACCATTTATGATGTGTGCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTCCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTATGGGTCTG  >  NC_000913/123344‑123607                                                                                                                                    |                                                                                                                                     tCCTTCCAGTCTTCCGCAACCATTTATGATGTGTGCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAg                                                                                                                               >  2:1245496/1‑139 (MQ=255)  ccTTCCAGTCTTCCGCAACCATTTATGATGTGTGCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAgg                                                                                                                              <  1:494647/139‑1 (MQ=255)    ttCCAGTCCTCCGCAACCATTTATGATGTGTGCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGgtc                                                                                                                            >  1:53398/1‑139 (MQ=255)             tCCGCAACCATTTATGATGTGTGCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTc                                                                                                                   >  2:466586/1‑139 (MQ=255)               cGCAACCATTTATGATGTGTGCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAgg                                                                                                                              <  1:1121192/126‑1 (MQ=255)               cGCAACCATTTATGATGTGTGCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAgg                                                                                                                              >  2:1121192/1‑126 (MQ=255)                    ccATTTATGATGTGTGCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGagca                                                                                                            <  2:53398/139‑1 (MQ=255)                               gtgtgCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACt                                                                                                 >  2:1020506/1‑139 (MQ=255)                                tgtgCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACtt                                                                                                >  1:659086/1‑139 (MQ=255)                                 gtgCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCt                                                                                                                                    <  1:66713/102‑1 (MQ=255)                                 gtgCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCt                                                                                                                                    >  2:66713/1‑102 (MQ=255)                                  tgCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGGGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGCGCAGCTGGATAACTTcc                                                                                              >  2:429622/1‑139 (MQ=255)                                  tgCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGgtcgt                                                                                                                          >  1:138668/1‑111 (MQ=255)                                  tgCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGgtcgt                                                                                                                          <  2:138668/111‑1 (MQ=255)                                  tgCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTcc                                                                                              >  1:594144/1‑139 (MQ=255)                                  tgCTTTAACCACTTCTTACGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTcc                                                                                              >  1:959945/1‑139 (MQ=255)                                   gCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCg                                                                                             <  2:386131/139‑1 (MQ=255)                                    cTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGt                                                                                            <  1:554618/139‑1 (MQ=255)                                       tAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAg                                                                                         >  1:88903/1‑139 (MQ=255)                                        aaCCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCAGATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAgg                                                                                        <  2:1101855/139‑1 (MQ=255)                                        aaCCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAgg                                                                                        >  1:398199/1‑139 (MQ=255)                                        aaCCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAgg                                                                                        <  1:1404224/139‑1 (MQ=255)                                          ccACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGaa                                                                                      >  1:133690/1‑139 (MQ=255)                                            aCTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGt                                                                                    >  2:1323918/1‑139 (MQ=255)                                              ttcttcCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCCGCTGGATAACTTCCGTCAGGAAGTTc                                                                                  >  2:549696/1‑139 (MQ=255)                                               tcttcCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCa                                                                                 >  1:452233/1‑139 (MQ=255)                                                      tGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGgtcg                                                                                                                           <  1:995841/90‑1 (MQ=255)                                                      tGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGgtcg                                                                                                                           >  2:995841/1‑90 (MQ=255)                                                       gCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATg                                                                         >  1:1512304/1‑139 (MQ=255)                                                         aCGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGc                                                                       <  2:959945/139‑1 (MQ=255)                                                         aCGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGc                                                                       <  2:88903/139‑1 (MQ=255)                                                         aCGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGc                                                                       <  2:659086/139‑1 (MQ=255)                                                           gCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCct                                                                     <  1:466586/139‑1 (MQ=255)                                                           gCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCct                                                                     <  2:1366979/139‑1 (MQ=255)                                                                     ggATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGa                                                                                                                      <  1:570837/80‑1 (MQ=255)                                                                     ggATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGa                                                                                                                      >  2:570837/1‑80 (MQ=255)                                                                       aTGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATcc                                                         >  2:1078611/1‑139 (MQ=255)                                                                                   tGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACt                                             <  1:1245496/139‑1 (MQ=255)                                                                                         aCTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGcc                                       <  2:29747/139‑1 (MQ=255)                                                                                          cTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCg                                      <  1:602243/139‑1 (MQ=255)                                                                                                gggCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGc                                                                       <  2:1239142/100‑1 (MQ=255)                                                                                                gggCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGc                                                                       >  1:1239142/1‑100 (MQ=255)                                                                                                   ccACATCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTgg                             <  2:452233/139‑1 (MQ=255)                                                                                                     acaTCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAgg                                                                                        <  1:214664/78‑1 (MQ=255)                                                                                                     acaTCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAgg                                                                                        >  2:214664/1‑78 (MQ=255)                                                                                                     acaTCTCCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCa                           <  1:1020506/139‑1 (MQ=255)                                                                                                          tcCCCGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTcc                      <  2:133690/139‑1 (MQ=255)                                                                                                           ccccGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGc                                                                       <  2:760642/89‑1 (MQ=255)                                                                                                           ccccGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGc                                                                       >  1:760642/1‑89 (MQ=255)                                                                                                           ccccGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTccc                     >  1:363705/1‑139 (MQ=255)                                                                                                             ccGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGa                                                                                                      <  2:1067283/56‑1 (MQ=255)                                                                                                             ccGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGa                                                                                                      >  1:1067283/1‑56 (MQ=255)                                                                                                              cGGGCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGAc                  <  2:363705/139‑1 (MQ=255)                                                                                                               gggCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGagcagc                                                                                                          <  2:1310935/50‑1 (MQ=255)                                                                                                               gggCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGagcagc                                                                                                          >  1:1310935/1‑50 (MQ=255)                                                                                                               gggCGTGTACGCTCGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGAcc                 <  2:398199/139‑1 (MQ=255)                                                                                                                            cGTGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAtgggtc    <  1:285682/139‑1 (MQ=255)                                                                                                                              tGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGa                   <  2:281194/122‑1 (MQ=255)                                                                                                                              tGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGa                   >  1:281194/1‑122 (MQ=255)                                                                                                                              tGCTTTTCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAtgggtctg  <  2:1512304/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     TCCTTCCAGTCTTCCGCAACCATTTATGATGTGTGCTTTAACCACTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGCCACATCTCCCCGGGCGTGTACGCTCGTGCTTTCCTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTATGGGTCTG  >  NC_000913/123344‑123607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑