| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,497,308 | A→G | E151E (GAA→GAG) | opgD → | OPG biosynthetic periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,497,308 | 0 | A | G | 98.2% | 187.6 / NA | 55 | E151E (GAA→GAG) | opgD | OPG biosynthetic periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (23/31); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAAT > NC_000913/1497184‑1497429 | tgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAgggg < 2:1389706/128‑1 (MQ=255)tgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAgggg > 1:1389706/1‑128 (MQ=255)tgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGAtct < 1:1321155/139‑1 (MQ=255) gggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGAtctc < 2:923744/139‑1 (MQ=255) cGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTg > 1:136215/1‑139 (MQ=255) gccgcGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCgg > 2:1251671/1‑139 (MQ=255) gCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTgg > 2:710091/1‑139 (MQ=255) agcTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGGGTTGATACAAACCAATTAGAGGGACAAAGAGATCGCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg < 2:318862/137‑1 (MQ=255) aTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg < 1:1380802/139‑1 (MQ=255) aTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTACAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg < 1:1229026/139‑1 (MQ=255) tCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGt < 1:1251671/139‑1 (MQ=255) cTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTg < 1:1232641/139‑1 (MQ=255) cTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTg < 1:107662/139‑1 (MQ=255) cTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTg < 1:1444844/139‑1 (MQ=255) tGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGa < 1:1025034/139‑1 (MQ=255) ggCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGc > 1:614266/1‑77 (MQ=255) ggCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGc < 2:614266/77‑1 (MQ=255) gcgcGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATg > 1:1440762/1‑139 (MQ=255) cgTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgtag < 2:136215/139‑1 (MQ=255) cgTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgtag > 1:1494705/1‑139 (MQ=255) tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgtagta > 1:106090/1‑139 (MQ=255) tttCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgc > 2:1259248/1‑90 (MQ=255) tttCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgc < 1:1259248/90‑1 (MQ=255) tCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgc > 2:542341/1‑139 (MQ=255) cGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg > 1:1399183/1‑139 (MQ=255) ttCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTcc > 2:1534334/1‑139 (MQ=255) cAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGAAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGACCTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgc < 2:1494705/139‑1 (MQ=255) aaaTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg < 1:456582/139‑1 (MQ=255) aaaTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg < 1:542341/139‑1 (MQ=255) aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGccc > 2:1471260/1‑83 (MQ=255) aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGccc < 1:1471260/83‑1 (MQ=255) aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg < 1:1430248/99‑1 (MQ=255) aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg > 2:1430248/1‑99 (MQ=255) aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc < 2:1399183/139‑1 (MQ=255) tACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGG‑TTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGt < 2:198967/139‑1 (MQ=255) aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga > 1:479534/1‑139 (MQ=255) aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga < 2:106090/139‑1 (MQ=255) aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga > 2:1415439/1‑139 (MQ=255) aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTACCGGTTTTCGCGTGTTTAAAGCCCCCg < 2:276058/81‑1 (MQ=255) aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTACCGGTTTTCGCGTGTTTAAAGCCCCCg > 1:276058/1‑81 (MQ=255) aTGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGa < 2:56486/94‑1 (MQ=255) aTGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGa > 1:56486/1‑94 (MQ=255) aTGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCAAACTGgcgcg < 2:1510126/88‑1 (MQ=255) aTGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCAAACTGgcgcg > 1:1510126/1‑88 (MQ=255) gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATa < 1:1415439/139‑1 (MQ=255) aGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACa < 1:1534334/139‑1 (MQ=255) ttGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgtag < 2:1043939/90‑1 (MQ=255) ttGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgtag > 1:1043939/1‑90 (MQ=255) gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcgc > 1:412696/1‑104 (MQ=255) gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcgc < 2:412696/104‑1 (MQ=255) gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAAt > 1:361222/1‑139 (MQ=255) aaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATa > 1:677494/1‑124 (MQ=255) aaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATa < 2:677494/124‑1 (MQ=255) aCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg < 1:733243/95‑1 (MQ=255) aCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg > 2:733243/1‑95 (MQ=255) ttAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgt > 1:374920/1‑74 (MQ=255) ttAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgt < 2:374920/74‑1 (MQ=255) | TGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAAT > NC_000913/1497184‑1497429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |