Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,533,045	C→T	intergenic (+93/‑7)	ydcC → / → pptA	H repeat‑associated putative transposase/4‑oxalocrotonate tautomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,533,045	0	C	T	100.0%	167.9 / NA	52	intergenic (+93/‑7)	ydcC/pptA	H repeat‑associated putative transposase/4‑oxalocrotonate tautomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/28);  total (24/28)

GCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCAT  >  NC_000913/1532920‑1533176                                                                                                                              |                                                                                                                                    gCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGc                                                                                                                        >  2:167866/1‑139 (MQ=255)      aGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATc                                                                                                                   <  1:1007420/139‑1 (MQ=255)                    gCGGGCTTTCGTAATCTTGCCCTGAGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGttt                                                                                                     >  2:1038432/1‑139 (MQ=255)                     cGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATGAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTGCTGACATCTTTCAAAAGGAGTGTAATCATGGCGCACATCGACATTAAATGtttt                                                                                                    >  2:1011691/1‑139 (MQ=255)                         cTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCcgc                                                                                                <  2:100709/139‑1 (MQ=255)                                       cccTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGaa                                                                                  <  1:1038432/139‑1 (MQ=255)                                                     gTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGTCGAACAACAAAAagcagc                                                                    <  1:1011691/139‑1 (MQ=255)                                                           taAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGa                                                                                   >  1:1049273/1‑118 (MQ=255)                                                           taAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGa                                                                                   <  2:1049273/118‑1 (MQ=255)                                                             aaCGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctg                                                            <  2:790149/139‑1 (MQ=255)                                                                gCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTaa                                                                                                                                 >  2:1399879/1‑67 (MQ=255)                                                                gCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTaa                                                                                                                                 <  1:1399879/67‑1 (MQ=255)                                                                   ttACCGGACACATGACAGTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGCGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGata                                                      >  1:960928/1‑139 (MQ=255)                                                                    tACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGa                                                        <  2:641709/136‑1 (MQ=255)                                                                    tACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGa                                                        >  1:641709/1‑136 (MQ=255)                                                                     aCCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATAtt                                                    >  2:723818/1‑139 (MQ=255)                                                                     aCCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATAtt                                                    >  1:871475/1‑139 (MQ=255)                                                                        ggACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTAcc                                                 <  2:871475/139‑1 (MQ=255)                                                                            acaTGACATTCACTATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACg                                             <  2:600713/139‑1 (MQ=255)                                                                            acaTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCcgcg                                                                                               <  2:835934/89‑1 (MQ=255)                                                                            acaTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCcgcg                                                                                               >  1:835934/1‑89 (MQ=255)                                                                                       acaATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGt                                  >  1:788914/1‑139 (MQ=255)                                                                                        caATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTc                                    <  2:864717/136‑1 (MQ=255)                                                                                        caATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTc                                    >  1:864717/1‑136 (MQ=255)                                                                                         gacaggttttAATTTTACTGACAGCTTTCAAAGGGAGTGTAACCTTGCCGCACTGCGACATTAAATG‑TTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCAt                               <  2:1010768/131‑1 (MQ=255)                                                                                          aTGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGCGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCAt                               <  2:960928/139‑1 (MQ=255)                                                                                           tgtgtgTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTc                                    <  1:439216/133‑1 (MQ=255)                                                                                           tgtgtgTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATc                              >  1:551082/1‑139 (MQ=255)                                                                                           tgtgtgTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATc                              >  2:1188360/1‑139 (MQ=255)                                                                                           tgtgtgTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATc                              >  2:841058/1‑139 (MQ=255)                                                                                           tgtgtgTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAAAGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTc                                    >  2:439216/1‑133 (MQ=255)                                                                                             tgtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCcgc                                                                                                <  1:494951/71‑1 (MQ=255)                                                                                             tgtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCcgc                                                                                                >  2:494951/1‑71 (MQ=255)                                                                                              gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa                           <  1:841058/139‑1 (MQ=255)                                                                                              gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa                           <  1:1188360/139‑1 (MQ=255)                                                                                              gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa                           <  2:551082/139‑1 (MQ=255)                                                                                               tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCGCATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa                           >  1:448545/1‑138 (MQ=255)                                                                                               tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCGCATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa                           <  2:448545/138‑1 (MQ=255)                                                                                               tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACg                                                                                    >  1:1237593/1‑81 (MQ=255)                                                                                               tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACg                                                                                    <  2:1237593/81‑1 (MQ=255)                                                                                               tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACg                                             <  2:542710/120‑1 (MQ=255)                                                                                               tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACg                                             >  1:542710/1‑120 (MQ=255)                                                                                               tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa                           <  2:505123/138‑1 (MQ=255)                                                                                               tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGa                           >  1:505123/1‑138 (MQ=255)                                                                                                gtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGa                                                                                   <  2:261781/81‑1 (MQ=255)                                                                                                gtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGa                                                                                   >  1:261781/1‑81 (MQ=255)                                                                                                       ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGa                                               <  2:1332050/110‑1 (MQ=255)                                                                                                       ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGa                                               >  1:1332050/1‑110 (MQ=255)                                                                                                       ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAg                  <  2:788914/139‑1 (MQ=255)                                                                                                         aCTGACATCTTTCAAAAGAAGTGTAATCATGCCGCACATCGACATTAAATG‑TTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACCTGCTGCAGATATTTCCCACGGTATTTTTCGTCAAATGAAAAATaagcgtc               >  1:1010768/1‑134 (MQ=255)                                                                                                                       aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCAt  <  2:763699/139‑1 (MQ=255)                                                                                                                       aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCAt  <  1:723818/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                    GCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCAT  >  NC_000913/1532920‑1533176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑