Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,557,112 C→T *309* (TAG→TAA)  ddpF ← D,D‑dipeptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,557,1120CT100.0% 134.7 / NA 43*309* (TAG→TAA) ddpFD,D‑dipeptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/20);  total (23/20)

ATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGT  >  NC_000913/1556976‑1557234
                                                                                                                                        |                                                                                                                          
aTCCAGAAAGCAAAAGCAGGATGTCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTa                                                                                                                          <  2:1033678/139‑1 (MQ=255)
                  ggATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg                                                                                                        <  2:19429/139‑1 (MQ=255)
                  ggATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg                                                                                                        >  1:1454169/1‑139 (MQ=255)
                  ggATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg                                                                                                        <  1:481745/139‑1 (MQ=255)
                             ctctcAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAg                                                                                                     <  2:175421/131‑1 (MQ=255)
                             ctctcAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAg                                                                                                     >  1:175421/1‑131 (MQ=255)
                                 cAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTGGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGAGAGGGAATGCCTGATGCGTTGTTAGCATCGTTACAGCGCCCGCCAGCACCGGAGATCAcgtccgtc                                                                                         <  2:1205565/139‑1 (MQ=255)
                                      aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc                                                                                         <  1:1254461/134‑1 (MQ=255)
                                      aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc                                                                                         >  2:1254461/1‑134 (MQ=255)
                                      aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTccc                                                                                    >  2:915941/1‑139 (MQ=255)
                                        tGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCtt                                                                                  >  1:1285025/1‑139 (MQ=255)
                                          aaaGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACgtccgtcc                                                                                        <  2:363777/131‑1 (MQ=255)
                                          aaaGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACgtccgtcc                                                                                        >  1:363777/1‑131 (MQ=255)
                                              cGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCt                                                                            >  2:705256/1‑139 (MQ=255)
                                                 aaaTTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAAt                                                                         <  2:1454169/139‑1 (MQ=255)
                                                         cTGGATTACCAGTTGAAATCGTAAAGCATTGCCGAATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTTTTAGCATCTTTACAGTGCCCGCCAGCACCGGAGCTCACGCCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                 <  2:786067/139‑1 (MQ=255)
                                                               tACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg                                                                                                                     <  1:1327832/81‑1 (MQ=255)
                                                               tACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg                                                                                                                     >  2:1327832/1‑81 (MQ=255)
                                                                aCCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGCATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTca                                                          <  1:915941/139‑1 (MQ=255)
                                                                 ccAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc                                                                                         >  1:435206/1‑107 (MQ=255)
                                                                 ccAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc                                                                                         <  2:435206/107‑1 (MQ=255)
                                                                      tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGCTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCGCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCg                                                    >  2:698041/1‑139 (MQ=255)
                                                                               aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGcc                                           >  2:228607/1‑139 (MQ=255)
                                                                                       gCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCa                                   >  2:954337/1‑139 (MQ=255)
                                                                                                cgcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCt                                                                                                   >  1:742731/1‑66 (MQ=255)
                                                                                                cgcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCt                                                                                                   <  2:742731/66‑1 (MQ=255)
                                                                                                cgcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTa                          >  1:1321339/1‑139 (MQ=255)
                                                                                                           cgTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGcc                                           >  1:133713/1‑111 (MQ=255)
                                                                                                           cgTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGcc                                           <  2:133713/111‑1 (MQ=255)
                                                                                                                  gCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCa                                   <  1:1212614/112‑1 (MQ=255)
                                                                                                                  gCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCa                                   >  2:1212614/1‑112 (MQ=255)
                                                                                                                    cTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc                                                                                         <  2:50694/56‑1 (MQ=255)
                                                                                                                    cTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc                                                                                         >  1:50694/1‑56 (MQ=255)
                                                                                                                      gATGCGTTGTTAGCATCTTTACAGCGCCCGCTAGCACCGGAGCTCAcgtccg                                                                                           >  2:1300226/1‑52 (MQ=255)
                                                                                                                      gATGCGTTGTTAGCATCTTTACAGCGCCCGCTAGCACCGGAGCTCAcgtccg                                                                                           <  1:1300226/52‑1 (MQ=255)
                                                                                                                      gATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAAc    >  2:11633/1‑139 (MQ=255)
                                                                                                                        tGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCgg                                      <  2:1056167/103‑1 (MQ=255)
                                                                                                                        tGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCgg                                      >  1:1056167/1‑103 (MQ=255)
                                                                                                                        tGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGt  >  1:785038/1‑139 (MQ=255)
                                                                                                                              gttAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTc                                                           >  2:157047/1‑76 (MQ=255)
                                                                                                                              gttAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTc                                                           <  1:157047/76‑1 (MQ=255)
                                                                                                                                    aTCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTca                                                          >  2:133059/1‑71 (MQ=255)
                                                                                                                                    aTCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTca                                                          <  1:133059/71‑1 (MQ=255)
                                                                                                                                        |                                                                                                                          
ATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGT  >  NC_000913/1556976‑1557234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑